(Circular) (Six-base) MAPSORT of: pFN21AE5533.seq Check: 2336 from: 1 to: 6106 pFN21AE5533 6106 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4169 5553 5821 1632 Size: 2537 1384 268 1917 Fragments arranged by size: 2537 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 5005 5982 1203 Size: 3802 977 1327 Fragments arranged by size: 3802 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 2902 4835 2902 Size: 1933 4173 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AlwNI CAG_nnn'CTG Cuts at: 3849 5421 3849 Size: 1572 4534 ApaI G_GGCC'C Cuts at: 2475 3021 2475 Size: 546 5560 ApaLI G'TGCA_C Cuts at: 4163 5319 4163 Size: 1156 4950 ApoI r'AATT_y Cuts at: 1493 2267 2333 3415 3641 3793 5902 1493 Size: 774 66 1082 226 152 2109 1697 Fragments arranged by size: 2109 1697 1082 774 226 152 66 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 3431 1870 Size: 73 1488 4545 Fragments arranged by size: 4545 1488 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2243 2276 2243 Size: 33 6073 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2378 3032 3427 4882 618 Size: 325 217 627 591 654 395 1455 1842 Fragments arranged by size: 1842 1455 654 627 591 395 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2475 2643 3021 3385 3425 1391 Size: 631 453 168 378 364 40 4072 Fragments arranged by size: 4072 631 453 378 364 168 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4297 4331 1903 Size: 34 50 34 2276 34 3678 Fragments arranged by size: 3678 2276 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4717 136 Size: 107 122 71 631 605 3045 1525 Fragments arranged by size: 3045 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 3523 3523 Size: 6106 Bme1580I G_kGCm'C Cuts at: 1790 2084 2205 2475 2771 3021 4167 5323 1790 Size: 294 121 270 296 250 1146 1156 2573 Fragments arranged by size: 2573 1156 1146 296 294 270 250 121 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2194 2753 3287 Size: 416 53 83 399 24 777 559 534 Cuts at: 3287 3837 4795 442 Size: 550 958 1753 Fragments arranged by size: 1753 958 777 559 550 534 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4766 1239 Size: 3527 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2519 2600 4231 5096 5394 2519 Size: 81 1631 865 298 3231 Fragments arranged by size: 3231 1631 865 298 81 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2130 3913 4295 4930 Size: 53 83 186 936 597 1783 382 635 Cuts at: 4930 275 Size: 1451 Fragments arranged by size: 1783 1451 936 635 597 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2704 4539 4937 5211 5358 5769 2704 Size: 1835 398 274 147 411 3041 Fragments arranged by size: 3041 1835 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5881 Fragments arranged by size: 5881 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2712 3068 1186 Size: 1526 356 4224 Fragments arranged by size: 4224 1526 356 BseYI C'CCAG_C Cuts at: 2204 3378 5309 5805 2204 Size: 1174 1931 496 2505 Fragments arranged by size: 2505 1931 1174 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5533 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 2643 2704 4167 4252 5323 2022 Size: 621 61 1463 85 1071 2805 Fragments arranged by size: 2805 1463 1071 621 85 61 BsmI GAATG_Cn' Cuts at: 2461 3617 3710 2461 Size: 1156 93 4857 Fragments arranged by size: 4857 1156 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2084 2205 2475 2643 2704 2771 Size: 399 232 62 121 270 168 61 67 Cuts at: 2771 3021 3385 3425 4167 4252 5323 1391 Size: 250 364 40 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 364 270 250 232 168 121 85 67 62 61 40 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 2410 3995 5725 2410 Size: 1585 1730 2791 Fragments arranged by size: 2791 1730 1585 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2535 2840 3461 844 Size: 1691 305 621 3489 Fragments arranged by size: 3489 1691 621 305 BsrBI CCG'CTC Cuts at: 1394 1823 2572 3993 1394 Size: 429 749 1421 3507 Fragments arranged by size: 3507 1421 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2630 4601 4775 65 Size: 1183 1382 1971 174 1396 Fragments arranged by size: 1971 1396 1382 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6106 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstXI CCAn_nnnn'nTGG Cuts at: 2874 3156 3300 2874 Size: 282 144 5680 Fragments arranged by size: 5680 282 144 BstYI r'GATC_y Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101 Size: 1464 367 385 17 1452 86 12 346 Cuts at: 6101 1972 Size: 1977 Fragments arranged by size: 1977 1464 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2670 2670 Size: 6106 BtgI C'CryG_G Cuts at: 513 1065 3283 513 Size: 552 2218 3336 Fragments arranged by size: 3336 2218 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 2842 3622 4416 4436 2842 Size: 780 794 20 4512 Fragments arranged by size: 4512 794 780 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3235 3553 4442 Size: 54 1425 50 275 18 1405 318 889 Cuts at: 4442 6071 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1405 889 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2579 4036 1353 Size: 1226 1457 3423 Fragments arranged by size: 3423 1457 1226 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2020 2641 2020 Size: 621 5485 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4169 4766 5553 5821 1239 Size: 393 2537 597 787 268 1524 Fragments arranged by size: 2537 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2471 3075 2471 Size: 604 5502 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2097 5253 1678 Size: 419 3156 2531 Fragments arranged by size: 3156 2531 419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2573 2605 2888 2920 4747 Size: 32 135 32 1001 32 283 32 1827 Cuts at: 4747 4779 4821 4853 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1827 1001 283 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 2949 3705 2949 Size: 756 5350 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2664 5220 5404 1305 Size: 1359 2556 184 2007 Fragments arranged by size: 2556 2007 1359 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3237 10 Size: 54 1425 343 1405 2879 Fragments arranged by size: 2879 1425 1405 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2872 4064 4423 4582 518 Size: 648 1706 1192 359 159 2042 Fragments arranged by size: 2042 1706 1192 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2140 3226 3522 4199 5347 5592 1606 Size: 534 1086 296 677 1148 245 2120 Fragments arranged by size: 2120 1148 1086 677 534 296 245 NcoI C'CATG_G Cuts at: 513 1065 3283 513 Size: 552 2218 3336 Fragments arranged by size: 3336 2218 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 3464 5009 1132 Size: 97 2235 1545 2229 Fragments arranged by size: 2235 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6106 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 2352 1108 Size: 1244 4862 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3075 3075 Size: 6106 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 2847 3676 2847 Size: 829 5277 PspOMI G'GGCC_C Cuts at: 2471 3017 2471 Size: 546 5560 PstI C_TGCA'G Cuts at: 830 2837 3458 830 Size: 2007 621 3478 Fragments arranged by size: 3478 2007 621 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 PvuII CAG'CTG Cuts at: 2140 3226 2140 Size: 1086 5020 SacI G_AGCT'C Cuts at: 2022 2643 2022 Size: 621 5485 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SapI GCTCTTCn'nnn_ Cuts at: 2579 2579 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SfcI C'TryA_G Cuts at: 826 1045 2833 2914 3042 3454 4589 5270 5461 Size: 219 1788 81 128 412 1135 681 191 Cuts at: 5461 826 Size: 1471 Fragments arranged by size: 1788 1471 1135 681 412 219 191 128 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2498 2579 4210 5111 5373 820 Size: 197 926 555 81 1631 901 262 1553 Fragments arranged by size: 1631 1553 926 901 555 262 197 81 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3464 1132 Size: 2332 3774 SspI AAT'ATT Cuts at: 5 52 2441 3013 4030 5 Size: 47 2389 572 1017 2081 Fragments arranged by size: 2389 2081 1017 572 47 StuI AGG'CCT Cuts at: 1517 2372 1517 Size: 855 5251 StyI C'CwwG_G Cuts at: 513 1065 3232 3283 3545 513 Size: 552 2167 51 262 3074 Fragments arranged by size: 3074 2167 552 262 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2541 4352 6064 Size: 274 80 33 51 1443 564 1811 1712 Cuts at: 6064 96 Size: 138 Fragments arranged by size: 1811 1712 1443 564 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6066 6098 6066 Size: 32 6074 Tth111I GACn'n_nGTC Cuts at: 1316 2486 3140 1316 Size: 1170 654 4282 Fragments arranged by size: 4282 1170 654 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3250 3250 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 2562 4235 2562 Size: 1673 4433 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI