################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI Fnu4HI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeI MaeII MaeIII MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 360 CCGC CCGC(-3/-1) AflIII 522 ACATGT A^C[GA][CT]GT AvaII 819 GGACC G^G[AT]CC BalI 194 TGGCCA TGG^CCA BanI 791 GGTGCC G^G[CT][GA]CC BanII 318 GAGCTC G[GA]GC[CT]^C BglII 247 AGATCT A^GATCT BpmI 481 CTGGAG CTGGAG(16/14) BseRI 536 GAGGAG GAGGAG(10/8) BsmFI 448 GTCCC GGGAC(10/14) BspLU11I 522 ACATGT A^CATGT BsrI 50 ACTGG ACTGG(1/-1) BstF5I 983 CATCC GGATG(2/0) BstXI 169 CCAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 500 GCGC GCG^C Cfr13I 819 GGACC G^G[ACGT]CC DsaI 191 CCATGG C^C[GA][CT]GG EaeI 194 TGGCCA [CT]^GGCC[GA] EarI 505 GAAGAG CTCTTC(1/4) EcoT22I 911 ATGCAT ATGCA^T FokI 983 CATCC GGATG(9/13) HaeII 499 GGCGCT [GA]GCGC^[CT] HaeIII 195 GGCC GG^CC HhaI 500 GCGC GCG^C HinP1I 500 GCGC G^CGC HinfI 979 GATTC G^A[ACGT]TC HphI 243 GGTGA GGTGA(8/7) Ksp632I 505 GAAGAG CTCTTC(1/4) MluNI 194 TGGCCA TGG^CCA MscI 194 TGGCCA TGG^CCA MslI 983 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 454 GCTGTTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 191 CCATGG C^CATGG NsiI 911 ATGCAT ATGCA^T NspI 522 ACATGT [GA]CATG^[CT] PstI 531 CTGCAG CTGCA^G SacI 318 GAGCTC GAGCT^C Sau96I 819 GGACC G^G[ACGT]CC SfcI 531 CTGCAG C^T[GA][CT]AG SspI 28 AATATT AAT^ATT TfiI 979 GATTC G^A[AT]TC XcmI 191 CCATGGCCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 379 GATCC GGATC(4/5) 607 GGATC AseI 25 ATTAAT AT^TAAT 666 ATTAAT AsnI 25 ATTAAT AT^TAAT 666 ATTAAT BsaBI 402 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 676 GATGCCAATC BsaJI 191 CCATGG C^C[ACGT][ACGT]GG 925 CCTTGG Bsp1407I 77 TGTACA T^GTACA 808 TGTACA BsrGI 77 TGTACA T^GTACA 808 TGTACA BssKI 604 CCTGG ^CC[ACGT]GG 986 CCAGG BstNI 604 CCTGG CC^[AT]GG 986 CCAGG BstYI 247 AGATCT [GA]^GATC[CT] 607 GGATCT EcoRII 604 CCTGG ^CC[AT]GG 986 CCAGG EcoT14I 191 CCATGG C^C[AT][AT]GG 925 CCTTGG MamI 402 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 676 GATGCCAATC MflI 247 AGATCT [GA]^GATC[CT] 607 GGATCT MvaI 604 CCTGG CC^[AT]GG 986 CCAGG NlaIV 791 GGTGCC GG[ACGT]^[ACGT]CC 819 GGACCC PshBI 25 ATTAAT AT^TAAT 666 ATTAAT ScrFI 604 CCTGG CC^[ACGT]GG 986 CCAGG SspBI 77 TGTACA T^GTACA 808 TGTACA StyI 191 CCATGG C^C[AT][AT]GG 925 CCTTGG XhoII 247 AGATCT [GA]^GATC[CT] 607 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AcsI AfaI AluI ApoI AspHI BbsI BlpI BmyI Bpu1102I BpuAI BsiHKAI Bsp1286I CelII DdeI DpnII MboI MboII MnlI MseI NdeII NlaIII RsaI Sau3AI SfaNI Tru9I Tsp509I TspRI