(Circular) (Six-base) MAPSORT of: pFN21AE4511.seq Check: 3096 from: 1 to: 6322 pFN21AE4511 6322 bp With 182 enzymes: SgfI * March 28, 2012 14:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2959 2959 Size: 6322 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2089 3665 1937 Size: 152 1576 4594 Fragments arranged by size: 4594 1576 152 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3158 4385 5769 6037 1632 Size: 1526 1227 1384 268 1917 Fragments arranged by size: 1917 1526 1384 1227 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 2826 5221 6198 1203 Size: 1623 2395 977 1327 Fragments arranged by size: 2395 1623 1327 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AlwNI CAG_nnn'CTG Cuts at: 4065 5637 4065 Size: 1572 4750 ApaI G_GGCC'C Cuts at: 2713 2839 2713 Size: 126 6196 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 2321 2405 2671 2719 2871 3480 3631 3857 Size: 828 84 266 48 152 609 151 226 Cuts at: 3857 4009 6118 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 828 609 266 226 152 152 151 84 48 AseI AT'TA_AT Cuts at: 160 1032 3402 4876 160 Size: 872 2370 1474 1606 Fragments arranged by size: 2370 1606 1474 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 3647 1870 Size: 73 1704 4545 Fragments arranged by size: 4545 1704 73 BamHI G'GATC_C Cuts at: 2209 3652 4019 2209 Size: 1443 367 4512 Fragments arranged by size: 4512 1443 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3643 5098 618 Size: 325 217 627 1856 1455 1842 Fragments arranged by size: 1856 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2713 2839 3121 3641 1391 Size: 1322 126 282 520 4072 Fragments arranged by size: 4072 1322 520 282 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4513 4547 1903 Size: 34 2576 34 3678 Fragments arranged by size: 3678 2576 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 2296 1548 Size: 748 5574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2708 4933 136 Size: 107 122 71 631 605 1036 2225 1525 Fragments arranged by size: 2225 1525 1036 631 605 122 107 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 2584 2713 2839 4383 5539 1790 Size: 794 129 126 1544 1156 2573 Fragments arranged by size: 2573 1544 1156 794 129 126 BmgBI CAC'GTC Cuts at: 1318 2827 1318 Size: 1509 4813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2520 4053 5011 Size: 416 53 83 399 24 1103 1533 958 Cuts at: 5011 442 Size: 1753 Fragments arranged by size: 1753 1533 1103 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2913 3228 4982 1239 Size: 1674 315 1754 2579 Fragments arranged by size: 2579 1754 1674 315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2061 4447 5312 5610 2061 Size: 2386 865 298 2773 Fragments arranged by size: 2773 2386 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4985 882 Size: 617 3486 2219 Fragments arranged by size: 3486 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4113 493 Size: 711 2909 2702 Fragments arranged by size: 2909 2702 711 BsaBI GATnn'nnATC Cuts at: 2528 6316 2528 Size: 3788 2534 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4129 4511 5146 275 Size: 53 83 186 936 2596 382 635 1451 Fragments arranged by size: 2596 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4755 5153 5427 5574 5985 4755 Size: 398 274 147 411 5092 Fragments arranged by size: 5092 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6097 Fragments arranged by size: 6097 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2733 1186 Size: 1547 4775 BseYI C'CCAG_C Cuts at: 3539 5525 6021 3539 Size: 1986 496 3840 Fragments arranged by size: 3840 1986 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5749 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3772 4533 5561 1540 Size: 275 186 1771 761 1028 2301 Fragments arranged by size: 2301 1771 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3121 3418 4383 4468 5539 3121 Size: 297 965 85 1071 3904 Fragments arranged by size: 3904 1071 965 297 85 BsmI GAATG_Cn' Cuts at: 2618 3833 3926 2618 Size: 1215 93 5014 Fragments arranged by size: 5014 1215 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2584 2713 2839 3121 3418 3641 4383 Size: 399 794 129 126 282 297 223 742 Cuts at: 4383 4468 5539 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 794 742 399 297 282 223 129 126 85 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 4211 5941 4211 Size: 1730 4592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2959 3677 844 Size: 2115 718 3489 Fragments arranged by size: 3489 2115 718 BsrBI CCG'CTC Cuts at: 1394 1823 4209 1394 Size: 429 2386 3507 Fragments arranged by size: 3507 2386 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4817 4991 65 Size: 1183 3569 174 1396 Fragments arranged by size: 3569 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4966 5153 1700 Size: 110 3156 187 2869 Fragments arranged by size: 3156 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2389 96 Size: 2293 4029 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 4382 5394 4382 Size: 1012 5310 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6322 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6322 BstYI r'GATC_y Cuts at: 1972 2209 3652 4019 4404 4421 5873 5959 5971 Size: 237 1443 367 385 17 1452 86 12 Cuts at: 5971 6317 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1443 385 367 346 237 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3552 3552 Size: 6322 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 4822 Fragments arranged by size: 4822 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 2809 3102 3838 4632 4652 2809 Size: 293 736 794 20 4479 Fragments arranged by size: 4479 794 736 293 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 3339 3626 3339 Size: 287 6035 DraIII CAC_nnn'GTG Cuts at: 3293 3293 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3769 4658 6287 Size: 54 1425 50 275 18 1939 889 1629 Cuts at: 6287 8 Size: 43 Fragments arranged by size: 1939 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3769 1537 Size: 275 1957 4090 Fragments arranged by size: 4090 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2089 4252 1353 Size: 736 2163 3423 Fragments arranged by size: 3423 2163 736 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4909 5283 5429 387 Size: 1064 138 3320 374 146 1280 Fragments arranged by size: 3320 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3119 3119 Size: 6322 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2913 3158 3228 4385 4982 5769 6037 Size: 393 1281 245 70 1157 597 787 268 Cuts at: 6037 1239 Size: 1524 Fragments arranged by size: 1524 1281 1157 787 597 393 268 245 70 EcoO109I rG'GnC_Cy Cuts at: 2710 2836 2710 Size: 126 6196 EcoRI G'AATT_C Cuts at: 3631 4009 3631 Size: 378 5944 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 5469 1678 Size: 3791 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4963 4995 5037 5069 1373 Size: 32 135 32 3391 32 42 32 2626 Fragments arranged by size: 3391 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6322 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5436 5620 1305 Size: 4131 184 2007 Fragments arranged by size: 4131 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4500 Fragments arranged by size: 4500 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2556 4280 4639 4798 518 Size: 648 1390 1724 359 159 2042 Fragments arranged by size: 2042 1724 1390 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3738 4415 5563 5808 1606 Size: 2132 677 1148 245 2120 Fragments arranged by size: 2132 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 4822 Fragments arranged by size: 4822 939 552 9 NdeI CA'TA_TG Cuts at: 387 3433 387 Size: 3046 3276 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 3680 5225 1132 Size: 97 2451 1545 2229 Fragments arranged by size: 2451 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6322 PciI A'CATG_T Cuts at: 5221 5221 Size: 6322 PflMI CCAn_nnn'nTGG Cuts at: 1108 3608 1108 Size: 2500 3822 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 2055 2937 3892 2055 Size: 882 955 4485 Fragments arranged by size: 4485 955 882 PspOMI G'GGCC_C Cuts at: 2709 2835 2709 Size: 126 6196 PstI C_TGCA'G Cuts at: 830 3674 830 Size: 2844 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 SacI G_AGCT'C Cuts at: 3121 3121 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SfcI C'TryA_G Cuts at: 826 1045 2038 2259 3670 4805 5486 5677 826 Size: 219 993 221 1411 1135 681 191 1471 Fragments arranged by size: 1471 1411 1135 993 681 221 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 3649 1872 Size: 1777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2076 4426 5327 5589 820 Size: 197 926 133 2350 901 262 1553 Fragments arranged by size: 2350 1553 926 901 262 197 133 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 3680 1132 Size: 2548 3774 SspI AAT'ATT Cuts at: 5 52 2593 2974 3148 3355 4246 5 Size: 47 2541 381 174 207 891 2081 Fragments arranged by size: 2541 2081 891 381 207 174 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6322 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 2493 3761 513 Size: 552 939 9 480 1268 3074 Fragments arranged by size: 3074 1268 939 552 480 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4349 4366 4519 4704 5149 325 Size: 1255 2769 17 153 185 445 1498 Fragments arranged by size: 2769 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2389 4568 6280 Size: 274 80 33 51 1443 412 2179 1712 Cuts at: 6280 96 Size: 138 Fragments arranged by size: 2179 1712 1443 412 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6282 6314 6282 Size: 32 6290 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6322 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6322 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 3647 1870 Size: 1777 4545 XmnI GAAnn'nnTTC Cuts at: 2559 2643 4451 2559 Size: 84 1808 4430 Fragments arranged by size: 4430 1808 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI