(Circular) (Six-base) MAPSORT of: pFN21AE4060.seq Check: 2318 from: 1 to: 5707 pFN21AE4060 5707 bp With 182 enzymes: SgfI * March 28, 2012 13:04 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3006 3834 4207 1563 Size: 1443 828 373 3063 Fragments arranged by size: 3063 1443 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2397 2600 3770 5154 5422 1632 Size: 765 203 1170 1384 268 1917 Fragments arranged by size: 1917 1384 1170 765 268 203 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 2143 2304 4436 2143 Size: 161 2132 3414 Fragments arranged by size: 3414 2132 161 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2161 2855 3450 5022 2161 Size: 694 595 1572 2846 Fragments arranged by size: 2846 1572 694 595 ApaI G_GGCC'C Cuts at: 2018 2018 Size: 5707 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2483 2998 3016 3242 3394 5503 1493 Size: 990 515 18 226 152 2109 1697 Fragments arranged by size: 2109 1697 990 515 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 3032 1870 Size: 73 1089 4545 Fragments arranged by size: 4545 1089 73 BamHI G'GATC_C Cuts at: 2245 3037 3404 2245 Size: 792 367 4548 Fragments arranged by size: 4548 792 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3028 4483 618 Size: 325 217 627 1241 1455 1842 Fragments arranged by size: 1842 1455 1241 627 325 217 BanII G_rGCy'C Cuts at: 1391 2018 2265 3026 1391 Size: 627 247 761 4072 Fragments arranged by size: 4072 761 627 247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2122 2890 928 Size: 971 223 768 3745 Fragments arranged by size: 3745 971 768 223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BfrBI ATG'CAT Cuts at: 2769 2769 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2053 2065 4318 Size: 107 122 71 631 605 381 12 2253 Cuts at: 4318 136 Size: 1525 Fragments arranged by size: 2253 1525 631 605 381 122 107 71 12 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 2018 3768 4924 1790 Size: 228 1750 1156 2573 Fragments arranged by size: 2573 1750 1156 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4396 442 Size: 416 53 83 399 24 2021 958 1753 Fragments arranged by size: 2021 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 4367 1239 Size: 944 2184 2579 Fragments arranged by size: 2579 2184 944 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2756 2948 3832 4697 4995 2756 Size: 192 884 865 298 3468 Fragments arranged by size: 3468 884 865 298 192 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4370 882 Size: 617 2871 2219 Fragments arranged by size: 2871 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 2244 5701 2244 Size: 3457 2250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2141 2162 2171 2192 1178 Size: 30 165 30 738 21 9 21 4693 Fragments arranged by size: 4693 738 165 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5707 BseYI C'CCAG_C Cuts at: 2062 2206 4910 5406 2062 Size: 144 2704 496 2363 Fragments arranged by size: 2704 2363 496 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2265 3768 3853 4924 2265 Size: 1503 85 1071 3048 Fragments arranged by size: 3048 1503 1071 85 BsmI GAATG_Cn' Cuts at: 2463 2629 3218 3311 2463 Size: 166 589 93 4859 Fragments arranged by size: 4859 589 166 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2966 2966 Size: 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2265 3026 3768 3853 4924 1391 Size: 399 228 247 761 742 85 1071 2174 Fragments arranged by size: 2174 1071 761 742 399 247 228 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 2354 2606 3062 844 Size: 1397 113 252 456 3489 Fragments arranged by size: 3489 1397 456 252 113 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2793 4202 4376 65 Size: 1183 1545 1409 174 1396 Fragments arranged by size: 1545 1409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 2706 96 Size: 2610 3097 BssHII G'CGCG_C Cuts at: 1925 2097 1925 Size: 172 5535 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 2158 2516 2673 794 Size: 1364 358 157 3828 Fragments arranged by size: 3828 1364 358 157 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2239 2245 3037 3404 3789 3806 5258 5344 Size: 267 6 792 367 385 17 1452 86 Cuts at: 5344 5356 5702 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 792 385 367 346 267 86 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 2004 2051 2400 513 Size: 552 939 47 349 3820 Fragments arranged by size: 3820 939 552 349 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 2848 3223 4017 4037 2848 Size: 375 794 20 4518 Fragments arranged by size: 4518 794 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3154 4043 5672 Size: 54 1425 50 275 18 1324 889 1629 Cuts at: 5672 8 Size: 43 Fragments arranged by size: 1629 1425 1324 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2363 3637 1353 Size: 1010 1274 3423 Fragments arranged by size: 3423 1274 1010 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2263 2263 Size: 5707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2397 2600 3770 4367 5154 5422 Size: 393 551 214 203 1170 597 787 268 Cuts at: 5422 1239 Size: 1524 Fragments arranged by size: 1524 1170 787 597 551 393 268 214 203 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2192 Size: 5707 EcoO109I rG'GnC_Cy Cuts at: 2210 2210 Size: 5707 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FspI TGC'GCA Cuts at: 2315 4213 2315 Size: 1898 3809 HaeII r_GCGC'y Cuts at: 1678 2322 4854 1678 Size: 644 2532 2531 Fragments arranged by size: 2532 2531 644 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2162 2194 2290 Size: 32 135 32 557 32 1 32 96 Cuts at: 2290 2322 4348 4380 4422 4454 1373 Size: 32 2026 32 42 32 2626 Fragments arranged by size: 2626 2026 557 135 96 42 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 2386 4821 5005 1305 Size: 822 259 2435 184 2007 Fragments arranged by size: 2435 2007 822 259 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3885 Fragments arranged by size: 3885 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2764 2774 3665 4024 4183 518 Size: 648 1598 10 891 359 159 2042 Fragments arranged by size: 2042 1598 891 648 359 159 10 MspA1I CmG'CkG Cuts at: 1606 2032 2062 2402 3123 3800 4948 5193 1606 Size: 426 30 340 721 677 1148 245 2120 Fragments arranged by size: 2120 1148 721 677 426 340 245 30 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NsiI A_TGCA'T Cuts at: 2771 2771 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 2092 1348 Size: 744 4963 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PspOMI G'GGCC_C Cuts at: 2014 2014 Size: 5707 PstI C_TGCA'G Cuts at: 830 2196 3059 830 Size: 1366 863 3478 Fragments arranged by size: 3478 1366 863 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 SacI G_AGCT'C Cuts at: 2265 2265 Size: 5707 SacII CC_GC'GG Cuts at: 2403 2403 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 2196 3059 2196 Size: 863 4844 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 2192 3055 4190 4871 5062 826 Size: 219 1147 863 1135 681 191 1471 Fragments arranged by size: 1471 1147 1135 863 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2735 2963 3811 4712 4974 820 Size: 197 926 792 228 848 901 262 1553 Fragments arranged by size: 1553 926 901 848 792 262 228 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 2989 3631 5 Size: 47 2937 642 2081 Fragments arranged by size: 2937 2081 642 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 3146 513 Size: 552 939 1142 3074 Fragments arranged by size: 3074 1142 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3734 3751 3904 4089 4534 325 Size: 1255 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 3953 5665 Size: 274 80 33 51 1443 729 1247 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1712 1443 1247 729 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2237 2269 5667 5699 2237 Size: 32 3398 32 2245 Fragments arranged by size: 3398 2245 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI