(Linear) (Six-base) MAPSORT of: KIEE4060.seq Check: 8173 from: 1 to: 1002 KIEE4060.orf With 182 enzymes: SgfI * March 22, 2012 17:17 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 392 595 1002 Size: 392 203 407 Fragments arranged by size: 407 392 203 AhdI GACnn_n'nnGTC Cuts at: 0 138 299 1002 Size: 138 161 703 Fragments arranged by size: 703 161 138 AlwNI CAG_nnn'CTG Cuts at: 0 156 850 1002 Size: 156 694 152 Fragments arranged by size: 694 156 152 ApaI G_GGCC'C Cuts at: 0 13 1002 Size: 13 989 ApoI r'AATT_y Cuts at: 0 478 993 1002 Size: 478 515 9 Fragments arranged by size: 515 478 9 BamHI G'GATC_C Cuts at: 0 240 1002 Size: 240 762 BanII G_rGCy'C Cuts at: 0 13 260 1002 Size: 13 247 742 Fragments arranged by size: 742 247 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 117 885 1002 Size: 117 768 117 Fragments arranged by size: 768 117 117 BfrBI ATG'CAT Cuts at: 0 764 1002 Size: 764 238 BglI GCCn_nnn'nGGC Cuts at: 0 48 60 1002 Size: 48 12 942 Fragments arranged by size: 942 48 12 Bme1580I G_kGCm'C Cuts at: 0 13 1002 Size: 13 989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 1002 Size: 178 824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 751 943 1002 Size: 751 192 59 Fragments arranged by size: 751 192 59 BsaBI GATnn'nnATC Cuts at: 0 239 1002 Size: 239 763 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 136 157 166 187 1002 Size: 136 21 9 21 815 Fragments arranged by size: 815 136 21 21 9 BseYI C'CCAG_C Cuts at: 0 57 201 1002 Size: 57 144 801 Fragments arranged by size: 801 144 57 BsiHKAI G_wGCw'C Cuts at: 0 260 1002 Size: 260 742 BsmI GAATG_Cn' Cuts at: 0 458 624 1002 Size: 458 166 378 Fragments arranged by size: 458 378 166 BsmBI CGTCTCn'nnnn_ Cuts at: 0 961 1002 Size: 961 41 Bsp1286I G_dGCh'C Cuts at: 0 13 260 1002 Size: 13 247 742 Fragments arranged by size: 742 247 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 236 349 601 1002 Size: 236 113 252 401 Fragments arranged by size: 401 252 236 113 BsrDI GCAATG_nn' Cuts at: 0 788 1002 Size: 788 214 BsrGI T'GTAC_A Cuts at: 0 701 1002 Size: 701 301 BssHII G'CGCG_C Cuts at: 0 92 1002 Size: 92 910 BstAPI GCAn_nnn'nTGC Cuts at: 0 153 511 668 1002 Size: 153 358 157 334 Fragments arranged by size: 358 334 157 153 BstYI r'GATC_y Cuts at: 0 234 240 1002 Size: 234 6 762 Fragments arranged by size: 762 234 6 BtgI C'CryG_G Cuts at: 0 46 395 1002 Size: 46 349 607 Fragments arranged by size: 607 349 46 BtsI GCAGTG_nn' Cuts at: 0 843 1002 Size: 843 159 EarI CTCTTCn'nnn_ Cuts at: 0 358 1002 Size: 358 644 EcoICRI GAG'CTC Cuts at: 0 258 1002 Size: 258 744 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 392 595 1002 Size: 178 214 203 407 Fragments arranged by size: 407 214 203 178 EcoNI CCTnn'n_nnAGG Cuts at: 0 187 1002 Size: 187 815 EcoO109I rG'GnC_Cy Cuts at: 0 205 1002 Size: 205 797 FspI TGC'GCA Cuts at: 0 310 1002 Size: 310 692 HaeII r_GCGC'y Cuts at: 0 317 1002 Size: 317 685 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 124 156 157 189 285 317 1002 Size: 124 32 1 32 96 32 685 Fragments arranged by size: 685 124 96 32 32 32 1 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 122 381 1002 Size: 122 259 621 Fragments arranged by size: 621 259 122 MslI CAynn'nnrTG Cuts at: 0 759 769 1002 Size: 759 10 233 Fragments arranged by size: 759 233 10 MspA1I CmG'CkG Cuts at: 0 27 57 397 1002 Size: 27 30 340 605 Fragments arranged by size: 605 340 30 27 NsiI A_TGCA'T Cuts at: 0 766 1002 Size: 766 236 PasI CC'CwG_GG Cuts at: 0 87 1002 Size: 87 915 PspOMI G'GGCC_C Cuts at: 0 9 1002 Size: 9 993 PstI C_TGCA'G Cuts at: 0 191 1002 Size: 191 811 SacI G_AGCT'C Cuts at: 0 260 1002 Size: 260 742 SacII CC_GC'GG Cuts at: 0 398 1002 Size: 398 604 SbfI CC_TGCA'GG Cuts at: 0 191 1002 Size: 191 811 SfcI C'TryA_G Cuts at: 0 187 1002 Size: 187 815 SmlI C'TyrA_G Cuts at: 0 730 958 1002 Size: 730 228 44 Fragments arranged by size: 730 228 44 SspI AAT'ATT Cuts at: 0 984 1002 Size: 984 18 TatI w'GTAC_w Cuts at: 0 701 1002 Size: 701 301 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 232 264 1002 Size: 232 32 738 Fragments arranged by size: 738 232 32 Enzymes that do cut: AcuI AhdI AlwNI ApaI ApoI BamHI BanII BbsI BfrBI BglI Bme1580I BpmI BpuEI BsaBI BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BssHII BstAPI BstYI BtgI BtsI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MmeI MslI MspA1I NsiI PasI PspOMI PstI SacI SacII SbfI SfcI SmlI SspI TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI