Restriction map for
pFN21AE9551
##################################
# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseRI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 452 TCCGGA T^CCGGA
AflIII 424 ACACGT A^C[GA][CT]GT
ApaI 278 GGGCCC GGGCC^C
AvaI 281 CCCGAG C^[CT]CG[GA]G
AvaII 240 GGTCC G^G[AT]CC
BanII 278 GGGCCC G[GA]GC[CT]^C
BciVI 336 GGATAC GTATCC(6/5)
BmyI 278 GGGCCC G[AGT]GC[ACT]^C
BsaWI 452 TCCGGA [AT]^CCGG[AT]
BseAI 452 TCCGGA T^CCGGA
BsgI 315 CTGCAC GTGCAG(16/14)
BsmAI 878 GTCTC GTCTC(1/5)
BsmFI 199 GTCCC GGGAC(10/14)
BsmI 254 GAATGC GAATGC(1/-1)
BsoBI 281 CCCGAG C^[CT]CG[GA]G
Bsp1286I 278 GGGCCC G[AGT]GC[ACT]^C
BspEI 452 TCCGGA T^CCGGA
BspHI 368 TCATGA T^CATGA
BsrDI 494 CATTGC GCAATG(2/0)
Bst1107I 588 GTATAC GTA^TAC
BstXI 243 CCACATAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 181 AGATCC [GA]^GATC[CT]
BstZ17I 588 GTATAC GTA^TAC
Cac8I 889 GCCAGC GC[ACGT]^[ACGT]GC
DraII 306 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 965 GAAGAG CTCTTC(1/4)
EcoO109I 306 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 14 ATGCAT ATGCA^T
HapII 453 CCGG C^CGG
HincII 744 GTTGAC GT[CT]^[GA]AC
HindII 744 GTTGAC GT[CT]^[GA]AC
HinfI 3 GAATC G^A[ACGT]TC
HpaII 453 CCGG C^CGG
Ksp632I 965 GAAGAG CTCTTC(1/4)
MaeIII 344 GTAAC ^GT[ACGT]AC
MboII 965 GAAGA GAAGA(8/7)
MflI 181 AGATCC [GA]^GATC[CT]
MroI 452 TCCGGA T^CCGGA
MspI 453 CCGG C^CGG
NsiI 14 ATGCAT ATGCA^T
RcaI 368 TCATGA T^CATGA
SapI 965 GAAGAGC GCTCTTC(1/4)
SfcI 357 CTGTAG C^T[GA][CT]AG
TfiI 3 GAATC G^A[AT]TC
XhoII 181 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 508 GTCTAC GT^[AC][GT]AC
588 GTATAC
AcsI 526 AAATTT [GA]^AATT[CT]
933 AAATTT
AluI 477 AGCT AG^CT
883 AGCT
AlwI 182 GATCC GGATC(4/5)
455 GGATC
AlwNI 197 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
702 CAGATGCTG
ApoI 526 AAATTT [GA]^AATT[CT]
933 AAATTT
BsaJI 282 CCGAGG C^C[ACGT][ACGT]GG
332 CCCTGG
BsiYI 116 CCATGATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
222 CCTGGCTGTGG
BslI 116 CCATGATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
222 CCTGGCTGTGG
DpnII 182 GATC ^GATC
456 GATC
DraI 45 TTTAAA TTT^AAA
529 TTTAAA
HphI 816 GGTGA GGTGA(8/7)
270 TCACC
MaeII 426 ACGT A^CGT
945 ACGT
MboI 182 GATC ^GATC
456 GATC
MwoI 355 GCCTGTAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
884 GCTATGCCAGC
NdeII 182 GATC ^GATC
456 GATC
NlaIV 278 GGGCCC GG[ACGT]^[ACGT]CC
306 GGGGCC
Sau3AI 182 GATC ^GATC
456 GATC
TaqI 417 TCGA T^CGA
514 TCGA
TthHB8I 417 TCGA T^CGA
514 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI BbvI BsrI BssKI BstF5I
BstNI Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII ItaI MnlI MseI
MvaI NlaIII RsaI Sau96I ScrFI
SfaNI Tru9I TseI Tsp509I TspRI