################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI HphI KasI KspI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 881 GGTACC G^GTACC AflIII 1179 ACATGT A^C[GA][CT]GT AhdI 289 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 477 ATTAAT AT^TAAT AsnI 477 ATTAAT AT^TAAT Asp718I 881 GGTACC G^GTACC AspEI 289 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1268 GAGCAC G[AT]GC[AT]^C BanI 881 GGTACC G^G[CT][GA]CC BbsI 190 GAAGAC GAAGAC(2/6) BcgI 126 GCAAGGTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 17 AGATCT A^GATCT BpuAI 190 GAAGAC GAAGAC(2/6) BsaBI 18 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 7 ACCGGA [AT]^CCGG[AT] BsgI 417 CTGCAC GTGCAG(16/14) BsiHKAI 1268 GAGCAC G[AT]GC[AT]^C BsmFI 1013 GTCCC GGGAC(10/14) BsmI 231 GAATGC GAATGC(1/-1) BspDI 865 ATCGAT AT^CGAT BspLU11I 1179 ACATGT A^CATGT BsrDI 435 GCAATG GCAATG(2/0) BstAPI 410 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 865 ATCGAT AT^CGAT DraI 562 TTTAAA TTT^AAA DsaI 962 CCATGG C^C[GA][CT]GG EaeI 794 CGGCCA [CT]^GGCC[GA] Eam1105I 289 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HapII 8 CCGG C^CGG HincII 371 GTCAAC GT[CT]^[GA]AC HindII 371 GTCAAC GT[CT]^[GA]AC HindIII 1219 AAGCTT A^AGCTT HpaII 8 CCGG C^CGG KpnI 881 GGTACC GGTAC^C MamI 18 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 8 CCGG C^CGG NcoI 962 CCATGG C^CATGG PflMI 1304 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 477 ATTAAT AT^TAAT Van91I 1304 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 740 CCACTGCAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 63 GCGG CCGC(-3/-1) 226 GCGG AfaI 131 GTAC GT^AC 882 GTAC BciVI 345 GTATCC GTATCC(6/5) 904 GTATCC BmyI 1254 GGGCAC G[AGT]GC[ACT]^C 1268 GAGCAC BsiYI 531 CCAATTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1304 CCAATTCCTGG BslI 531 CCAATTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1304 CCAATTCCTGG Bsp1286I 1254 GGGCAC G[AGT]GC[ACT]^C 1268 GAGCAC BspMI 633 ACCTGC ACCTGC(4/8) 971 GCAGGT BstYI 17 AGATCT [GA]^GATC[CT] 1040 GGATCT MaeII 894 ACGT A^CGT 1022 ACGT MflI 17 AGATCT [GA]^GATC[CT] 1040 GGATCT NlaIV 881 GGTACC GG[ACGT]^[ACGT]CC 1400 GGGGCC RsaI 131 GTAC GT^AC 882 GTAC SfcI 41 CTACAG C^T[GA][CT]AG 168 CTGTAG SphI 913 GCATGC GCATG^C 996 GCATGC Tsp45I 211 GTCAC ^GT[GC]AC 260 GTGAC XhoII 17 AGATCT [GA]^GATC[CT] 1040 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AcsI AluI AlwI ApoI AvaII BbvI BfaI BsaJI BseRI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NspI PleI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I