################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 97 AAATTT [GA]^AATT[CT] AlwI 315 GATCC GGATC(4/5) AocI 340 CCTGAGG CC^T[ACGT]AGG ApoI 97 AAATTT [GA]^AATT[CT] Asp700I 651 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 266 GAGCCC G[GA]GC[CT]^C BclI 368 TGATCA T^GATCA BlpI 456 GCTGAGC GC^T[ACGT]AGC BmyI 266 GAGCCC G[AGT]GC[ACT]^C Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC BseRI 502 GAGGAG GAGGAG(10/8) BsgI 196 GTGCAG GTGCAG(16/14) BsiYI 53 CCACCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 53 CCACCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 266 GAGCCC G[AGT]GC[ACT]^C BspMI 569 ACCTGC ACCTGC(4/8) BsrDI 222 CATTGC GCAATG(2/0) BsrI 515 CCAGT ACTGG(1/-1) Bsu36I 340 CCTGAGG CC^T[ACGT]AGG CelII 456 GCTGAGC GC^T[ACGT]AGC Cfr13I 546 GGCCC G^G[ACGT]CC DraII 545 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 54 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 91 GAAGAG CTCTTC(1/4) Eco81I 340 CCTGAGG CC^T[ACGT]AGG EcoO109I 545 AGGCCCT [GA]G^G[ACGT]CC[CT] FbaI 368 TGATCA T^GATCA HaeIII 546 GGCC GG^CC HindIII 358 AAGCTT A^AGCTT HphI 307 GGTGA GGTGA(8/7) Ksp632I 91 GAAGAG CTCTTC(1/4) MaeII 497 ACGT A^CGT MspA1I 74 CAGCTG C[AC]G^C[GT]G MwoI 355 GCCAAGCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 62 GGCTCC GG[ACGT]^[ACGT]CC PflMI 53 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 511 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 74 CAGCTG CAG^CTG Sau96I 546 GGCCC G^G[ACGT]CC SfaNI 352 GATGC GCATC(5/9) TaqI 500 TCGA T^CGA TfiI 560 GAATC G^A[AT]TC Tsp509I 98 AATT ^AATT TspRI 578 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 500 TCGA T^CGA Van91I 53 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 651 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BcnI 56 CCCGG CC^[GC]GG 462 CCGGG BstF5I 185 GGATG GGATG(2/0) 624 GGATG BstNI 66 CCTGG CC^[AT]GG 598 CCTGG Cac8I 226 GCCAGC GC[ACGT]^[ACGT]GC 360 GCTTGC DdeI 341 CTGAG C^T[ACGT]AG 457 CTGAG DpnII 315 GATC ^GATC 369 GATC DsaI 426 CCATGG C^C[GA][CT]GG 620 CCATGG EcoRII 66 CCTGG ^CC[AT]GG 598 CCTGG FokI 185 GGATG GGATG(9/13) 624 GGATG HapII 57 CCGG C^CGG 462 CCGG HpaII 57 CCGG C^CGG 462 CCGG MboI 315 GATC ^GATC 369 GATC MseI 130 TTAA T^TAA 418 TTAA MspI 57 CCGG C^CGG 462 CCGG MvaI 66 CCTGG CC^[AT]GG 598 CCTGG NciI 56 CCCGG CC^[GC]GG 462 CCGGG NcoI 426 CCATGG C^CATGG 620 CCATGG NdeII 315 GATC ^GATC 369 GATC NlaIII 427 CATG CATG^ 621 CATG PleI 511 GACTC GAGTC(4/5) 49 GAGTC PstI 71 CTGCAG CTGCA^G 110 CTGCAG Sau3AI 315 GATC ^GATC 369 GATC Tru9I 130 TTAA T^TAA 418 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BbvI BpmI BsaJI BssKI EcoT14I Fnu4HI HinfI ItaI MboII MnlI ScrFI SfcI StyI TseI