################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspHI BspLU11I BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HinfI HpaI KasI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MscI MseI MspA1I MunI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 275 GGTACC G^GTACC AccII 199 CGCG CG^CG AccIII 389 TCCGGA T^CCGGA AcsI 354 GAATTC [GA]^AATT[CT] ApoI 354 GAATTC [GA]^AATT[CT] Asp718I 275 GGTACC G^GTACC AvaII 264 GGACC G^G[AT]CC BanI 275 GGTACC G^G[CT][GA]CC BsaAI 212 CACGTA [CT]AC^GT[GA] BsaI 376 GAGACC GGTCTC(1/5) BsaWI 389 TCCGGA [AT]^CCGG[AT] BseAI 389 TCCGGA T^CCGGA BsmAI 376 GAGAC GTCTC(1/5) BsmFI 263 GGGAC GGGAC(10/14) Bsp1407I 291 TGTACA T^GTACA BspEI 389 TCCGGA T^CCGGA BspMI 457 ACCTGC ACCTGC(4/8) BsrBI 228 CCGCTC CCGCTC(-3/-3) BsrGI 291 TGTACA T^GTACA BstUI 199 CGCG CG^CG CfoI 198 GCGC GCG^C DraII 263 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 478 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 29 CCATGG C^C[GA][CT]GG EcoNI 145 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 263 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 354 GAATTC G^AATTC FauI 141 CCCGC CCCGC(4/6) HapII 390 CCGG C^CGG HgaI 461 GCGTC GACGC(5/10) HhaI 198 GCGC GCG^C HinP1I 198 GCGC G^CGC HindIII 166 AAGCTT A^AGCTT HpaII 390 CCGG C^CGG KpnI 275 GGTACC GGTAC^C MaeII 213 ACGT A^CGT MroI 389 TCCGGA T^CCGGA MslI 345 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 390 CCGG C^CGG MvnI 199 CGCG CG^CG MwoI 452 GCTGCACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 29 CCATGG C^CATGG PpuMI 263 GGGACCC [GA]G^G[AT]CC[CT] PshAI 378 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 38 AGTACT AGT^ACT SmlI 329 CTCAAG C^T[CT][GA]AG SspBI 291 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AluI 87 AGCT AG^CT 167 AGCT BbsI 9 GAAGAC GAAGAC(2/6) 340 GAAGAC BpuAI 9 GAAGAC GAAGAC(2/6) 340 GAAGAC BsgI 453 CTGCAC GTGCAG(16/14) 484 GTGCAG BsrI 300 CCAGT ACTGG(1/-1) 423 CCAGT BstF5I 366 CATCC GGATG(2/0) 474 CATCC Cac8I 63 GCTTGC GC[ACGT]^[ACGT]GC 140 GCCCGC DdeI 73 CTGAG C^T[ACGT]AG 131 CTGAG EcoT14I 29 CCATGG C^C[AT][AT]GG 402 CCAAGG FokI 366 CATCC GGATG(9/13) 474 CATCC HaeIII 22 GGCC GG^CC 297 GGCC HphI 441 GGTGA GGTGA(8/7) 464 TCACC MboII 9 GAAGA GAAGA(8/7) 340 GAAGA SfaNI 179 GCATC GCATC(5/9) 320 GCATC StyI 29 CCATGG C^C[AT][AT]GG 402 CCAAGG TaqI 52 TCGA T^CGA 221 TCGA Tsp509I 247 AATT ^AATT 355 AATT TthHB8I 52 TCGA T^CGA 221 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AfaI BbvI BsaJI BsiYI BslI BssKI BstNI Cfr13I EcoRII Fnu4HI ItaI MaeIII MnlI MvaI NlaIII NlaIV RsaI Sau96I ScrFI TseI Tsp45I TspRI