(Circular) (Six-base) MAPSORT of: pFN21AB5806.seq Check: 2007 from: 1 to: 6061 pFN21AB5806 6061 bp With 182 enzymes: SgfI * March 28, 2012 13:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3303 4124 5508 5776 1632 Size: 1671 821 1384 268 1917 Fragments arranged by size: 1917 1671 1384 821 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 2612 2728 1166 Size: 1446 116 4499 Fragments arranged by size: 4499 1446 116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 3326 3804 5376 3326 Size: 478 1572 4011 Fragments arranged by size: 4011 1572 478 ApaI G_GGCC'C Cuts at: 2502 2502 Size: 6061 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 3044 3370 3596 3748 5857 1493 Size: 1551 326 226 152 2109 1697 Fragments arranged by size: 2109 1697 1551 326 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2907 3386 1870 Size: 73 964 479 4545 Fragments arranged by size: 4545 964 479 73 BamHI G'GATC_C Cuts at: 3083 3391 3758 3083 Size: 308 367 5386 Fragments arranged by size: 5386 367 308 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2606 2712 3382 4837 Size: 325 217 627 372 447 106 670 1455 Cuts at: 4837 618 Size: 1842 Fragments arranged by size: 1842 1455 670 627 447 372 325 217 106 BanII G_rGCy'C Cuts at: 1391 2334 2502 2546 3380 1391 Size: 943 168 44 834 4072 Fragments arranged by size: 4072 943 834 168 44 BbeI G_GCGC'C Cuts at: 2716 2716 Size: 6061 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2848 928 Size: 971 949 4141 Fragments arranged by size: 4141 971 949 BbvCI CC'TCA_GC Cuts at: 2700 2700 Size: 6061 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2888 2922 4252 4286 1903 Size: 34 951 34 1330 34 3678 Fragments arranged by size: 3678 1330 951 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 3147 1548 Size: 1599 4462 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2502 4122 5278 1790 Size: 712 1620 1156 2573 Fragments arranged by size: 2573 1620 1156 712 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2551 3089 3792 Size: 416 53 83 399 24 1134 538 703 Cuts at: 3792 4750 442 Size: 958 1753 Fragments arranged by size: 1753 1134 958 703 538 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 3073 4721 1239 Size: 875 959 1648 2579 Fragments arranged by size: 2579 1648 959 875 Bpu10I CC'TnA_GC Cuts at: 2700 2700 Size: 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2354 3079 4186 5051 5349 2354 Size: 725 1107 865 298 3066 Fragments arranged by size: 3066 1107 865 725 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3852 493 Size: 711 2648 2702 Fragments arranged by size: 2702 2648 711 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2268 2713 3868 4250 Size: 53 83 186 936 735 445 1155 382 Cuts at: 4250 4885 275 Size: 635 1451 Fragments arranged by size: 1451 1155 936 735 635 445 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 1178 Size: 30 165 30 1140 30 4666 Fragments arranged by size: 4666 1140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2119 2122 1186 Size: 933 3 5125 Fragments arranged by size: 5125 933 3 BseYI C'CCAG_C Cuts at: 2404 2501 5264 5760 2404 Size: 97 2763 496 2705 Fragments arranged by size: 2763 2705 496 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2336 3201 1163 Size: 573 600 865 4023 Fragments arranged by size: 4023 865 600 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2256 3511 4272 5300 1540 Size: 275 186 255 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 275 255 186 BsiHKAI G_wGCw'C Cuts at: 4122 4207 5278 4122 Size: 85 1071 4905 Fragments arranged by size: 4905 1071 85 BsmI GAATG_Cn' Cuts at: 2475 3572 3665 2475 Size: 1097 93 4871 Fragments arranged by size: 4871 1097 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2334 2502 2546 3380 4122 4207 5278 Size: 399 544 168 44 834 742 85 1071 Cuts at: 5278 1391 Size: 2174 Fragments arranged by size: 2174 1071 834 742 544 399 168 85 44 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 2445 3950 5680 2445 Size: 1505 1730 2826 Fragments arranged by size: 2826 1730 1505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 3416 844 Size: 1514 1058 3489 Fragments arranged by size: 3489 1514 1058 BsrBI CCG'CTC Cuts at: 1394 1823 2209 3263 3948 1394 Size: 429 386 1054 685 3507 Fragments arranged by size: 3507 1054 685 429 386 BsrDI GCAATG_nn' Cuts at: 65 1248 2694 4556 4730 65 Size: 1183 1446 1862 174 1396 Fragments arranged by size: 1862 1446 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 4705 4892 1700 Size: 110 346 2549 187 2869 Fragments arranged by size: 2869 2549 346 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 3179 794 Size: 2385 3676 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 2689 1184 Size: 1505 4556 BstYI r'GATC_y Cuts at: 1972 2316 3083 3391 3758 4143 4160 5612 5698 Size: 344 767 308 367 385 17 1452 86 Cuts at: 5698 5710 6056 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 767 385 367 346 344 308 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2323 2452 2520 513 Size: 552 1258 129 68 4054 Fragments arranged by size: 4054 1258 552 129 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 3577 4371 4391 3577 Size: 794 20 5247 Fragments arranged by size: 5247 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2514 2578 Size: 54 1425 50 275 18 423 261 64 Cuts at: 2578 2650 3274 3508 4397 6026 8 Size: 72 624 234 889 1629 43 Fragments arranged by size: 1629 1425 889 624 423 275 261 234 72 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2253 3508 1537 Size: 275 441 1255 4090 Fragments arranged by size: 4090 1255 441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2593 2761 3991 1353 Size: 1240 168 1230 3423 Fragments arranged by size: 3423 1240 1230 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3273 3281 4648 5022 5168 387 Size: 1064 138 1684 8 1367 374 146 1280 Fragments arranged by size: 1684 1367 1280 1064 374 146 138 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 3073 3303 4124 4721 5508 5776 Size: 393 482 959 230 821 597 787 268 Cuts at: 5776 1239 Size: 1524 Fragments arranged by size: 1524 959 821 787 597 482 393 268 230 EcoO109I rG'GnC_Cy Cuts at: 2498 2498 Size: 6061 EcoRI G'AATT_C Cuts at: 3044 3370 3748 3044 Size: 326 378 5357 Fragments arranged by size: 5357 378 326 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 2369 2716 5208 1678 Size: 691 347 2492 2531 Fragments arranged by size: 2531 2492 691 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2258 2290 4702 Size: 32 135 32 584 32 70 32 2412 Cuts at: 4702 4734 4776 4808 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2412 584 135 70 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2620 3405 3651 669 Size: 988 281 72 610 785 246 3079 Fragments arranged by size: 3079 988 785 610 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KasI G'GCGC_C Cuts at: 2712 2712 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2580 3276 10 Size: 54 1425 343 748 696 2795 Fragments arranged by size: 2795 1425 748 696 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2612 2728 4019 4378 4537 518 Size: 648 1446 116 1291 359 159 2042 Fragments arranged by size: 2042 1446 1291 648 359 159 116 MspA1I CmG'CkG Cuts at: 1606 2522 2793 3477 4154 5302 5547 1606 Size: 916 271 684 677 1148 245 2120 Fragments arranged by size: 2120 1148 916 684 677 271 245 NarI GG'CG_CC Cuts at: 2713 2713 Size: 6061 NcoI C'CATG_G Cuts at: 513 1065 2452 513 Size: 552 1387 4122 Fragments arranged by size: 4122 1387 552 NdeI CA'TA_TG Cuts at: 387 2979 387 Size: 2592 3469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 3287 3419 4964 1132 Size: 97 2058 132 1545 2229 Fragments arranged by size: 2229 2058 1545 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6061 PfoI T'CCnGG_A Cuts at: 2020 2020 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 2498 2498 Size: 6061 PstI C_TGCA'G Cuts at: 830 3413 830 Size: 2583 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 SacII CC_GC'GG Cuts at: 2523 2523 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SapI GCTCTTCn'nnn_ Cuts at: 2761 2761 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 2306 4309 6021 2306 Size: 2003 1712 2346 Fragments arranged by size: 2346 2003 1712 SfcI C'TryA_G Cuts at: 826 1045 3409 4544 5225 5416 826 Size: 219 2364 1135 681 191 1471 Fragments arranged by size: 2364 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2714 2714 Size: 6061 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2369 3058 4165 5066 5328 820 Size: 197 926 426 689 1107 901 262 1553 Fragments arranged by size: 1553 1107 926 901 689 426 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 2026 152 Size: 1874 4187 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2452 2581 2877 3277 3500 513 Size: 552 1387 129 296 400 223 3074 Fragments arranged by size: 3074 1387 552 400 296 223 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2490 4088 4105 4258 4443 4888 325 Size: 1255 910 1598 17 153 185 445 1498 Fragments arranged by size: 1598 1498 1255 910 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 2304 4307 Size: 274 80 33 51 1443 222 105 2003 Cuts at: 4307 6019 96 Size: 1712 138 Fragments arranged by size: 2003 1712 1443 274 222 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 2543 2575 6021 6053 2204 Size: 32 307 32 3446 32 2212 Fragments arranged by size: 3446 2212 307 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3106 3397 3106 Size: 291 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2509 2977 2509 Size: 468 5593 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI