(Circular) (Six-base) MAPSORT of: pFN21AE9456.seq Check: 1427 from: 1 to: 6790 pFN21AE9456 6790 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3227 3227 Size: 6790 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4600 278 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4133 1937 Size: 2196 4594 Acc65I G'GTAC_C Cuts at: 4111 4111 Size: 6790 AclI AA'CG_TT Cuts at: 1563 4917 5290 1563 Size: 3354 373 3063 Fragments arranged by size: 3354 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 4034 4853 6237 6505 1632 Size: 510 1892 819 1384 268 1917 Fragments arranged by size: 1917 1892 1384 819 510 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6593 AflIII A'CryG_T Cuts at: 1203 2234 2270 3324 3417 5689 6666 1203 Size: 1031 36 1054 93 2272 977 1327 Fragments arranged by size: 2272 1327 1054 1031 977 93 36 AgeI A'CCGG_T Cuts at: 5621 5621 Size: 6790 AhdI GACnn_n'nnGTC Cuts at: 5519 5519 Size: 6790 AleI CACnn'nnGTG Cuts at: 1166 3149 1166 Size: 1983 4807 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6790 AlwNI CAG_nnn'CTG Cuts at: 4533 6105 4533 Size: 1572 5218 ApaLI G'TGCA_C Cuts at: 4847 6003 4847 Size: 1156 5634 ApoI r'AATT_y Cuts at: 1493 2659 4099 4325 4477 6586 1493 Size: 1166 1440 226 152 2109 1697 Fragments arranged by size: 2109 1697 1440 1166 226 152 AseI AT'TA_AT Cuts at: 160 1032 4073 5344 160 Size: 872 3041 1271 1606 Fragments arranged by size: 3041 1606 1271 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 AvaI C'yCGr_G Cuts at: 1870 1943 2541 3288 4115 1870 Size: 73 598 747 827 4545 Fragments arranged by size: 4545 827 747 598 73 BamHI G'GATC_C Cuts at: 2145 4120 4487 2145 Size: 1975 367 4448 Fragments arranged by size: 4448 1975 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3576 4111 5566 618 Size: 325 217 627 1789 535 1455 1842 Fragments arranged by size: 1842 1789 1455 627 535 325 217 BanII G_rGCy'C Cuts at: 1391 2377 3046 3231 4109 1391 Size: 986 669 185 878 4072 Fragments arranged by size: 4072 986 878 669 185 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2930 3999 928 Size: 971 1031 1069 3719 Fragments arranged by size: 3719 1069 1031 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4981 5015 1903 Size: 34 3044 34 3678 Fragments arranged by size: 3678 3044 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4682 5892 4682 Size: 1210 5580 BclI T'GATC_A Cuts at: 1548 3264 3585 1548 Size: 1716 321 4753 Fragments arranged by size: 4753 1716 321 BfrBI ATG'CAT Cuts at: 2864 4043 2864 Size: 1179 5611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3698 5401 136 Size: 107 122 71 631 605 2026 1703 1525 Fragments arranged by size: 2026 1703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6785 6785 Size: 6790 BlpI GC'TnA_GC Cuts at: 3230 4207 3230 Size: 977 5813 Bme1580I G_kGCm'C Cuts at: 1790 3581 4851 6007 1790 Size: 1791 1270 1156 2573 Fragments arranged by size: 2573 1791 1270 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6790 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2617 3040 3670 Size: 416 53 83 399 24 1200 423 630 Cuts at: 3670 4521 5479 442 Size: 851 958 1753 Fragments arranged by size: 1753 1200 958 851 630 423 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6790 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2415 2415 Size: 6790 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2121 2361 5450 1239 Size: 882 240 3089 2579 Fragments arranged by size: 3089 2579 882 240 Bpu10I CC'TnA_GC Cuts at: 2205 2843 3245 2205 Size: 638 402 5750 Fragments arranged by size: 5750 638 402 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2385 4915 5780 6078 2385 Size: 2530 865 298 3097 Fragments arranged by size: 3097 2530 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2375 3631 5453 882 Size: 617 876 1256 1822 2219 Fragments arranged by size: 2219 1822 1256 876 617 BsaAI yAC'GTr Cuts at: 493 1204 3325 4581 493 Size: 711 2121 1256 2702 Fragments arranged by size: 2702 2121 1256 711 BsaBI GATnn'nnATC Cuts at: 6784 6784 Size: 6790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4597 4979 5614 275 Size: 53 83 186 936 3064 382 635 1451 Fragments arranged by size: 3064 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5223 5621 5895 6042 6453 5223 Size: 398 274 147 411 5560 Fragments arranged by size: 5560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6565 Fragments arranged by size: 6565 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6790 BseYI C'CCAG_C Cuts at: 3844 5993 6489 3844 Size: 2149 496 4145 Fragments arranged by size: 4145 2149 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6217 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4240 5001 6029 1540 Size: 275 186 2239 761 1028 2301 Fragments arranged by size: 2301 2239 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2377 4851 4936 6007 2377 Size: 2474 85 1071 3160 Fragments arranged by size: 3160 2474 1071 85 BsmI GAATG_Cn' Cuts at: 4045 4301 4394 4045 Size: 256 93 6441 Fragments arranged by size: 6441 256 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3241 3241 Size: 6790 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2377 3046 3231 3581 4109 4851 4936 Size: 399 587 669 185 350 528 742 85 Cuts at: 4936 6007 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 669 587 528 399 350 185 85 BspEI T'CCGG_A Cuts at: 6453 6453 Size: 6790 BspHI T'CATG_A Cuts at: 4679 6409 4679 Size: 1730 5060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3227 4145 844 Size: 2383 918 3489 Fragments arranged by size: 3489 2383 918 BsrBI CCG'CTC Cuts at: 1394 1823 4677 1394 Size: 429 2854 3507 Fragments arranged by size: 3507 2854 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3040 3488 3588 5285 5459 65 Size: 1183 1792 448 100 1697 174 1396 Fragments arranged by size: 1792 1697 1396 1183 448 174 100 BsrFI r'CCGG_y Cuts at: 1700 1810 5434 5621 1700 Size: 110 3624 187 2869 Fragments arranged by size: 3624 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2273 96 Size: 2177 4613 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6790 BssSI C'ACGA_G Cuts at: 4850 5862 4850 Size: 1012 5778 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6790 BstBI TT'CG_AA Cuts at: 3012 5594 3012 Size: 2582 4208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6790 BstXI CCAn_nnnn'nTGG Cuts at: 3053 3053 Size: 6790 BstYI r'GATC_y Cuts at: 1972 2078 2145 4064 4120 4487 4872 4889 6341 Size: 106 67 1919 56 367 385 17 1452 Cuts at: 6341 6427 6439 6785 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1919 1452 385 367 346 106 86 67 56 17 12 Bsu36I CC'TnA_GG Cuts at: 3642 3642 Size: 6790 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6238 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6051 Fragments arranged by size: 6051 683 56 BtsI GCAGTG_nn' Cuts at: 3351 3435 3839 4306 5100 5120 3351 Size: 84 404 467 794 20 5021 Fragments arranged by size: 5021 794 467 404 84 20 ClaI AT'CG_AT Cuts at: 1554 3188 1554 Size: 1634 5156 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2231 2266 529 Size: 35 1667 35 5053 Fragments arranged by size: 5053 1667 35 35 DraI TTT'AAA Cuts at: 4094 4094 Size: 6790 DraIII CAC_nnn'GTG Cuts at: 3282 3282 Size: 6790 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5797 809 Size: 507 4481 1802 Fragments arranged by size: 4481 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2855 4237 Size: 54 1425 50 275 18 741 284 1382 Cuts at: 4237 5126 6755 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1382 889 741 284 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4237 1537 Size: 275 2425 4090 Fragments arranged by size: 4090 2425 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2549 2723 3485 4720 1353 Size: 1196 174 762 1235 3423 Fragments arranged by size: 3423 1235 1196 762 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5377 5751 5897 387 Size: 1064 138 3788 374 146 1280 Fragments arranged by size: 3788 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2375 2375 Size: 6790 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2142 2361 4034 4853 5450 6237 Size: 393 489 21 219 1673 819 597 787 Cuts at: 6237 6505 1239 Size: 268 1524 Fragments arranged by size: 1673 1524 819 787 597 489 393 268 219 21 EcoO109I rG'GnC_Cy Cuts at: 2165 3762 2165 Size: 1597 5193 EcoRI G'AATT_C Cuts at: 4099 4477 4099 Size: 378 6412 EcoRV GAT'ATC Cuts at: 2809 3112 3309 2809 Size: 303 197 6290 Fragments arranged by size: 6290 303 197 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4005 4005 Size: 6790 FspI TGC'GCA Cuts at: 5296 5296 Size: 6790 HaeII r_GCGC'y Cuts at: 1678 5937 1678 Size: 4259 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4028 4060 5431 5463 5505 Size: 32 135 32 2456 32 1371 32 42 Cuts at: 5505 5537 1373 Size: 32 2626 Fragments arranged by size: 2626 2456 1371 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3751 4134 4380 669 Size: 988 281 1813 383 246 3079 Fragments arranged by size: 3079 1813 988 383 281 246 HindIII A'AGCT_T Cuts at: 748 3247 748 Size: 2499 4291 HpaI GTT'AAC Cuts at: 4380 4380 Size: 6790 KpnI G_GTAC'C Cuts at: 4115 4115 Size: 6790 MfeI C'AATT_G Cuts at: 4389 4389 Size: 6790 MluI A'CGCG_T Cuts at: 6666 6666 Size: 6790 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 3087 5904 6088 1305 Size: 903 879 2817 184 2007 Fragments arranged by size: 2817 2007 903 879 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2573 2857 10 Size: 54 1425 343 741 284 3943 Fragments arranged by size: 3943 1425 741 343 284 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2447 2786 3149 3173 3803 4748 Size: 648 843 438 339 363 24 630 945 Cuts at: 4748 5107 5266 518 Size: 359 159 2042 Fragments arranged by size: 2042 945 843 648 630 438 363 359 339 159 24 MspA1I CmG'CkG Cuts at: 1606 2264 3135 4034 4206 4883 6031 6276 1606 Size: 658 871 899 172 677 1148 245 2120 Fragments arranged by size: 2120 1148 899 871 677 658 245 172 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6238 NdeI CA'TA_TG Cuts at: 387 387 Size: 6790 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6790 NotI GC'GGCC_GC Cuts at: 4237 4237 Size: 6790 NsiI A_TGCA'T Cuts at: 2866 4045 2866 Size: 1179 5611 NspI r_CATG'y Cuts at: 1132 1229 2238 2274 3319 3421 4148 5693 1132 Size: 97 1009 36 1045 102 727 1545 2229 Fragments arranged by size: 2229 1545 1045 1009 727 102 97 36 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6790 PciI A'CATG_T Cuts at: 2234 2270 3417 5689 2234 Size: 36 1147 2272 3335 Fragments arranged by size: 3335 2272 1147 36 PflMI CCAn_nnn'nTGG Cuts at: 1108 3282 3369 4061 1108 Size: 2174 87 692 3837 Fragments arranged by size: 3837 2174 692 87 PmeI GTTT'AAAC Cuts at: 4094 4094 Size: 6790 PmlI CAC'GTG Cuts at: 3325 3325 Size: 6790 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4859 4891 6372 6404 4859 Size: 32 1481 32 5245 Fragments arranged by size: 5245 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2165 2165 Size: 6790 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6790 PsiI TTA'TAA Cuts at: 4360 4360 Size: 6790 PstI C_TGCA'G Cuts at: 830 2192 4142 830 Size: 1362 1950 3478 Fragments arranged by size: 3478 1950 1362 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6790 PvuII CAG'CTG Cuts at: 2264 4034 2264 Size: 1770 5020 SacI G_AGCT'C Cuts at: 2377 2377 Size: 6790 SalI G'TCGA_C Cuts at: 1936 4132 1936 Size: 2196 4594 SapI GCTCTTCn'nnn_ Cuts at: 2549 3485 2549 Size: 936 5854 SbfI CC_TGCA'GG Cuts at: 4142 4142 Size: 6790 ScaI AGT'ACT Cuts at: 5038 6750 5038 Size: 1712 5078 SfcI C'TryA_G Cuts at: 826 1045 2074 2188 2331 2517 2560 3600 4138 Size: 219 1029 114 143 186 43 1040 538 Cuts at: 4138 5273 5954 6145 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1040 1029 681 538 219 191 186 143 114 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6790 SmaI CCC'GGG Cuts at: 1872 3290 4117 1872 Size: 1418 827 4545 Fragments arranged by size: 4545 1418 827 SmlI C'TyrA_G Cuts at: 820 1017 1943 2400 2541 4894 5795 6057 820 Size: 197 926 457 141 2353 901 262 1553 Fragments arranged by size: 2353 1553 926 901 457 262 197 141 SnaBI TAC'GTA Cuts at: 493 4581 493 Size: 4088 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6790 SphI G_CATG'C Cuts at: 1132 4148 1132 Size: 3016 3774 SspI AAT'ATT Cuts at: 5 52 2482 2512 3510 4714 5 Size: 47 2430 30 998 1204 2081 Fragments arranged by size: 2430 2081 1204 998 47 30 StuI AGG'CCT Cuts at: 1517 3647 1517 Size: 2130 4660 StyI C'CwwG_G Cuts at: 513 1065 2895 4229 513 Size: 552 1830 1334 3074 Fragments arranged by size: 3074 1830 1334 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3209 3564 4817 4834 4987 5172 5617 Size: 1255 1629 355 1253 17 153 185 445 Cuts at: 5617 325 Size: 1498 Fragments arranged by size: 1629 1498 1255 1253 445 355 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2273 5036 6748 Size: 274 80 33 51 1443 296 2763 1712 Cuts at: 6748 96 Size: 138 Fragments arranged by size: 2763 1712 1443 296 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2063 2095 6750 6782 2063 Size: 32 4655 32 2071 Fragments arranged by size: 4655 2071 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6790 XbaI T'CTAG_A Cuts at: 2362 4126 2362 Size: 1764 5026 XhoI C'TCGA_G Cuts at: 1943 2541 1943 Size: 598 6192 XmaI C'CCGG_G Cuts at: 1870 3288 4115 1870 Size: 1418 827 4545 Fragments arranged by size: 4545 1418 827 XmnI GAAnn'nnTTC Cuts at: 4919 4919 Size: 6790 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4598 276 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI