(Linear) (Six-base) MAPSORT of: KIEE9456.seq Check: 6341 from: 1 to: 2085 KIEE9456.orf With 182 enzymes: SgfI * December 27, 2011 16:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1222 2085 Size: 1222 863 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 2029 2085 Size: 137 1892 56 Fragments arranged by size: 1892 137 56 AflIII A'CryG_T Cuts at: 0 229 265 1319 1412 2085 Size: 229 36 1054 93 673 Fragments arranged by size: 1054 673 229 93 36 AleI CACnn'nnGTG Cuts at: 0 1144 2085 Size: 1144 941 ApoI r'AATT_y Cuts at: 0 654 2085 Size: 654 1431 AseI AT'TA_AT Cuts at: 0 2068 2085 Size: 2068 17 AvaI C'yCGr_G Cuts at: 0 536 1283 2085 Size: 536 747 802 Fragments arranged by size: 802 747 536 BamHI G'GATC_C Cuts at: 0 140 2085 Size: 140 1945 BanI G'GyrC_C Cuts at: 0 1571 2085 Size: 1571 514 BanII G_rGCy'C Cuts at: 0 372 1041 1226 2085 Size: 372 669 185 859 Fragments arranged by size: 859 669 372 185 BbsI GAAGACnn'nnnn_ Cuts at: 0 925 1994 2085 Size: 925 1069 91 Fragments arranged by size: 1069 925 91 BclI T'GATC_A Cuts at: 0 1259 1580 2085 Size: 1259 321 505 Fragments arranged by size: 1259 505 321 BfrBI ATG'CAT Cuts at: 0 859 2038 2085 Size: 859 1179 47 Fragments arranged by size: 1179 859 47 BglI GCCn_nnn'nGGC Cuts at: 0 1693 2085 Size: 1693 392 BlpI GC'TnA_GC Cuts at: 0 1225 2085 Size: 1225 860 Bme1580I G_kGCm'C Cuts at: 0 1576 2085 Size: 1576 509 BmrI ACTGGGnnnn_n' Cuts at: 0 612 1035 1665 2085 Size: 612 423 630 420 Fragments arranged by size: 630 612 423 420 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 410 2085 Size: 410 1675 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 356 2085 Size: 116 240 1729 Fragments arranged by size: 1729 240 116 Bpu10I CC'TnA_GC Cuts at: 0 200 838 1240 2085 Size: 200 638 402 845 Fragments arranged by size: 845 638 402 200 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 2085 Size: 380 1705 BsaI GGTCTCn'nnnn_ Cuts at: 0 370 1626 2085 Size: 370 1256 459 Fragments arranged by size: 1256 459 370 BsaAI yAC'GTr Cuts at: 0 1320 2085 Size: 1320 765 BseYI C'CCAG_C Cuts at: 0 1839 2085 Size: 1839 246 BsiHKAI G_wGCw'C Cuts at: 0 372 2085 Size: 372 1713 BsmI GAATG_Cn' Cuts at: 0 2040 2085 Size: 2040 45 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1236 2085 Size: 1236 849 Bsp1286I G_dGCh'C Cuts at: 0 372 1041 1226 1576 2085 Size: 372 669 185 350 509 Fragments arranged by size: 669 509 372 350 185 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1222 2085 Size: 1222 863 BsrDI GCAATG_nn' Cuts at: 0 1035 1483 1583 2085 Size: 1035 448 100 502 Fragments arranged by size: 1035 502 448 100 BsrGI T'GTAC_A Cuts at: 0 268 2085 Size: 268 1817 BstBI TT'CG_AA Cuts at: 0 1007 2085 Size: 1007 1078 BstXI CCAn_nnnn'nTGG Cuts at: 0 1048 2085 Size: 1048 1037 BstYI r'GATC_y Cuts at: 0 73 140 2059 2085 Size: 73 67 1919 26 Fragments arranged by size: 1919 73 67 26 Bsu36I CC'TnA_GG Cuts at: 0 1637 2085 Size: 1637 448 BtsI GCAGTG_nn' Cuts at: 0 1346 1430 1834 2085 Size: 1346 84 404 251 Fragments arranged by size: 1346 404 251 84 ClaI AT'CG_AT Cuts at: 0 1183 2085 Size: 1183 902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 226 261 2085 Size: 226 35 1824 Fragments arranged by size: 1824 226 35 DraIII CAC_nnn'GTG Cuts at: 0 1277 2085 Size: 1277 808 EaeI y'GGCC_r Cuts at: 0 566 850 2085 Size: 566 284 1235 Fragments arranged by size: 1235 566 284 EarI CTCTTCn'nnn_ Cuts at: 0 544 718 1480 2085 Size: 544 174 762 605 Fragments arranged by size: 762 605 544 174 EcoICRI GAG'CTC Cuts at: 0 370 2085 Size: 370 1715 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 137 356 2029 2085 Size: 116 21 219 1673 56 Fragments arranged by size: 1673 219 116 56 21 EcoO109I rG'GnC_Cy Cuts at: 0 160 1757 2085 Size: 160 1597 328 Fragments arranged by size: 1597 328 160 EcoRV GAT'ATC Cuts at: 0 804 1107 1304 2085 Size: 804 303 197 781 Fragments arranged by size: 804 781 303 197 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2000 2085 Size: 2000 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2023 2055 2085 Size: 2023 32 30 Fragments arranged by size: 2023 32 30 HincII GTy'rAC Cuts at: 0 1746 2085 Size: 1746 339 HindIII A'AGCT_T Cuts at: 0 1242 2085 Size: 1242 843 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 203 1082 2085 Size: 203 879 1003 Fragments arranged by size: 1003 879 203 MscI TGG'CCA Cuts at: 0 568 852 2085 Size: 568 284 1233 Fragments arranged by size: 1233 568 284 MslI CAynn'nnrTG Cuts at: 0 442 781 1144 1168 1798 2085 Size: 442 339 363 24 630 287 Fragments arranged by size: 630 442 363 339 287 24 MspA1I CmG'CkG Cuts at: 0 259 1130 2029 2085 Size: 259 871 899 56 Fragments arranged by size: 899 871 259 56 NsiI A_TGCA'T Cuts at: 0 861 2040 2085 Size: 861 1179 45 Fragments arranged by size: 1179 861 45 NspI r_CATG'y Cuts at: 0 233 269 1314 1416 2085 Size: 233 36 1045 102 669 Fragments arranged by size: 1045 669 233 102 36 PciI A'CATG_T Cuts at: 0 229 265 1412 2085 Size: 229 36 1147 673 Fragments arranged by size: 1147 673 229 36 PflMI CCAn_nnn'nTGG Cuts at: 0 1277 1364 2056 2085 Size: 1277 87 692 29 Fragments arranged by size: 1277 692 87 29 PmlI CAC'GTG Cuts at: 0 1320 2085 Size: 1320 765 PpuMI rG'GwC_Cy Cuts at: 0 160 2085 Size: 160 1925 PstI C_TGCA'G Cuts at: 0 187 2085 Size: 187 1898 PvuII CAG'CTG Cuts at: 0 259 2029 2085 Size: 259 1770 56 Fragments arranged by size: 1770 259 56 SacI G_AGCT'C Cuts at: 0 372 2085 Size: 372 1713 SapI GCTCTTCn'nnn_ Cuts at: 0 544 1480 2085 Size: 544 936 605 Fragments arranged by size: 936 605 544 SfcI C'TryA_G Cuts at: 0 69 183 326 512 555 1595 2085 Size: 69 114 143 186 43 1040 490 Fragments arranged by size: 1040 490 186 143 114 69 43 SmaI CCC'GGG Cuts at: 0 1285 2085 Size: 1285 800 SmlI C'TyrA_G Cuts at: 0 395 536 2085 Size: 395 141 1549 Fragments arranged by size: 1549 395 141 SspI AAT'ATT Cuts at: 0 477 507 1505 2085 Size: 477 30 998 580 Fragments arranged by size: 998 580 477 30 StuI AGG'CCT Cuts at: 0 1642 2085 Size: 1642 443 StyI C'CwwG_G Cuts at: 0 890 2085 Size: 890 1195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1204 1559 2085 Size: 1204 355 526 Fragments arranged by size: 1204 526 355 TatI w'GTAC_w Cuts at: 0 268 2085 Size: 268 1817 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 58 90 2085 Size: 58 32 1995 Fragments arranged by size: 1995 58 32 XbaI T'CTAG_A Cuts at: 0 357 2085 Size: 357 1728 XhoI C'TCGA_G Cuts at: 0 536 2085 Size: 536 1549 XmaI C'CCGG_G Cuts at: 0 1283 2085 Size: 1283 802 Enzymes that do cut: AarI AcuI AflIII AleI ApoI AseI AvaI BamHI BanI BanII BbsI BclI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BstBI BstXI BstYI Bsu36I BtsI ClaI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NsiI NspI PciI PflMI PmlI PpuMI PstI PvuII SacI SapI SfcI SmaI SmlI SspI StuI StyI TaqII TatI TstI XbaI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BglII BmgBI BmtI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstZ17I BtgI BtgZI DraI DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XcmI XmnI ZraI