Restriction map for
pFN21AE9453
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BcnI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 2362 CGCG CG^CG
AflII 2565 CTTAAG C^TTAAG
AseI 472 ATTAAT AT^TAAT
AsnI 472 ATTAAT AT^TAAT
Asp700I 568 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1079 TGGCCA TGG^CCA
BanII 2401 GGGCTC G[GA]GC[CT]^C
BbvCI 417 CCTCAGC CCTCAGC(-5/-2)
BclI 1811 TGATCA T^GATCA
BfrI 2565 CTTAAG C^TTAAG
BsaWI 2051 ACCGGA [AT]^CCGG[AT]
BseRI 2377 CTCCTC GAGGAG(10/8)
BsmI 2482 GCATTC GAATGC(1/-1)
BspLU11I 684 ACATGT A^CATGT
BspMI 876 GCAGGT ACCTGC(4/8)
BssSI 2062 CTCGTG CACGAG(-5/-1)
BstBI 608 TTCGAA TT^CGAA
BstUI 2362 CGCG CG^CG
BstXI 223 CCATTATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1417 AGATCC [GA]^GATC[CT]
DraIII 1648 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1515 GATATC GAT^ATC
FauI 1182 GCGGG CCCGC(4/6)
FbaI 1811 TGATCA T^GATCA
HapII 2052 CCGG C^CGG
HincII 1452 GTTGAC GT[CT]^[GA]AC
HindII 1452 GTTGAC GT[CT]^[GA]AC
HpaII 2052 CCGG C^CGG
MflI 1417 AGATCC [GA]^GATC[CT]
MluNI 1079 TGGCCA TGG^CCA
MscI 1079 TGGCCA TGG^CCA
MspI 2052 CCGG C^CGG
MvnI 2362 CGCG CG^CG
NdeI 563 CATATG CA^TATG
NspV 608 TTCGAA TT^CGAA
PshBI 472 ATTAAT AT^TAAT
SexAI 328 ACCTGGT A^CC[AT]GGT
SfuI 608 TTCGAA TT^CGAA
SspI 1060 AATATT AAT^ATT
XhoII 1417 AGATCC [GA]^GATC[CT]
XmnI 568 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 2361 CCGC CCGC(-3/-1)
1182 GCGG
AfaI 308 GTAC GT^AC
1688 GTAC
AflIII 684 ACATGT A^C[GA][CT]GT
1864 ACACGT
AspHI 383 GAGCAC G[AT]GC[AT]^C
898 GTGCTC
AspI 775 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2330 GACTGAGTC
BbsI 316 GAAGAC GAAGAC(2/6)
733 GAAGAC
BpuAI 316 GAAGAC GAAGAC(2/6)
733 GAAGAC
BsgI 1968 CTGCAC GTGCAG(16/14)
2249 CTGCAC
BsiHKAI 383 GAGCAC G[AT]GC[AT]^C
898 GTGCTC
BsrDI 2151 CATTGC GCAATG(2/0)
947 GCAATG
BstEII 159 GGTGACC G^GT[ACGT]ACC
597 GGTCACC
BstPI 159 GGTGACC G^GT[ACGT]ACC
597 GGTCACC
EaeI 48 CGGCCA [CT]^GGCC[GA]
1079 TGGCCA
EarI 1256 CTCTTC CTCTTC(1/4)
2370 GAAGAG
EcoNI 2177 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2392 CCTCTGCTAGG
EcoO65I 159 GGTGACC G^GT[ACGT]ACC
597 GGTCACC
EcoRI 501 GAATTC G^AATTC
572 GAATTC
EcoT14I 176 CCAAGG C^C[AT][AT]GG
797 CCAAGG
EcoT22I 2480 ATGCAT ATGCA^T
2666 ATGCAT
Ksp632I 1256 CTCTTC CTCTTC(1/4)
2370 GAAGAG
MaeII 1678 ACGT A^CGT
1866 ACGT
NsiI 2480 ATGCAT ATGCA^T
2666 ATGCAT
NspI 684 ACATGT [GA]CATG^[CT]
2457 GCATGT
PflFI 775 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2330 GACTGAGTC
PflMI 2070 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGAATCTGG
PstI 301 CTGCAG CTGCA^G
822 CTGCAG
RsaI 308 GTAC GT^AC
1688 GTAC
SapI 2370 GAAGAGC GCTCTTC(1/4)
1255 GCTCTTC
SmlI 1664 CTCAAG C^T[CT][GA]AG
2565 CTTAAG
StyI 176 CCAAGG C^C[AT][AT]GG
797 CCAAGG
Tth111I 775 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2330 GACTGAGTC
Van91I 2070 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGAATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
AvaII BanI BbvI BfaI BglI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI