(Circular) (Six-base) MAPSORT of: pFN21AE9453.seq Check: 5489 from: 1 to: 7435 pFN21AE9453 7435 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5245 278 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4778 1937 Size: 2841 4594 Acc65I G'GTAC_C Cuts at: 4756 4756 Size: 7435 AclI AA'CG_TT Cuts at: 1563 5562 5935 1563 Size: 3999 373 3063 Fragments arranged by size: 3999 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2231 5498 6882 7150 1632 Size: 512 87 3267 1384 268 1917 Fragments arranged by size: 3267 1917 1384 512 268 87 AflII C'TTAA_G Cuts at: 820 1017 4570 820 Size: 197 3553 3685 Fragments arranged by size: 3685 3553 197 AflIII A'CryG_T Cuts at: 1203 2689 3869 6334 7311 1203 Size: 1486 1180 2465 977 1327 Fragments arranged by size: 2465 1486 1327 1180 977 AgeI A'CCGG_T Cuts at: 6266 6266 Size: 7435 AhdI GACnn_n'nnGTC Cuts at: 6164 6164 Size: 7435 AleI CACnn'nnGTG Cuts at: 1166 3245 3773 3953 1166 Size: 2079 528 180 4648 Fragments arranged by size: 4648 2079 528 180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3263 1184 Size: 2079 5356 AlwNI CAG_nnn'CTG Cuts at: 2155 3983 4453 5178 6750 2155 Size: 1828 470 725 1572 2840 Fragments arranged by size: 2840 1828 1572 725 470 ApaLI G'TGCA_C Cuts at: 5492 6648 5492 Size: 1156 6279 ApoI r'AATT_y Cuts at: 1493 2203 2506 2550 2577 2932 3271 3284 4628 Size: 710 303 44 27 355 339 13 1344 Cuts at: 4628 4744 4970 5122 7231 1493 Size: 116 226 152 2109 1697 Fragments arranged by size: 2109 1697 1344 710 355 339 303 226 152 116 44 27 13 AseI AT'TA_AT Cuts at: 160 1032 2478 5989 160 Size: 872 1446 3511 1606 Fragments arranged by size: 3511 1606 1446 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 AvaI C'yCGr_G Cuts at: 1870 1943 4760 1870 Size: 73 2817 4545 Fragments arranged by size: 4545 2817 73 BamHI G'GATC_C Cuts at: 4765 5132 4765 Size: 367 7068 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3247 3935 4128 4756 6211 Size: 325 217 627 1460 688 193 628 1455 Cuts at: 6211 618 Size: 1842 Fragments arranged by size: 1842 1460 1455 688 628 627 325 217 193 BanII G_rGCy'C Cuts at: 1391 4410 4754 1391 Size: 3019 344 4072 Fragments arranged by size: 4072 3019 344 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 2745 928 Size: 971 429 417 5618 Fragments arranged by size: 5618 971 429 417 BbvCI CC'TCA_GC Cuts at: 2423 2423 Size: 7435 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5626 5660 1903 Size: 34 3689 34 3678 Fragments arranged by size: 3689 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5327 6537 5327 Size: 1210 6225 BclI T'GATC_A Cuts at: 1548 3816 1548 Size: 2268 5167 BfrBI ATG'CAT Cuts at: 4487 4673 4487 Size: 186 7249 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2061 2702 2963 Size: 107 122 71 631 605 389 641 261 Cuts at: 2963 6046 136 Size: 3083 1525 Fragments arranged by size: 3083 1525 641 631 605 389 261 122 107 71 BglII A'GATC_T Cuts at: 7430 7430 Size: 7435 BlpI GC'TnA_GC Cuts at: 4852 4852 Size: 7435 Bme1580I G_kGCm'C Cuts at: 1790 2468 4133 5496 6652 1790 Size: 678 1665 1363 1156 2573 Fragments arranged by size: 2573 1665 1363 1156 678 BmgBI CAC'GTC Cuts at: 1318 3684 3872 1318 Size: 2366 188 4881 Fragments arranged by size: 4881 2366 188 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2129 2764 3791 Size: 416 53 83 399 24 712 635 1027 Cuts at: 3791 5166 6124 442 Size: 1375 958 1753 Fragments arranged by size: 1753 1375 1027 958 712 635 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7435 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3615 3615 Size: 7435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2406 2638 3987 4333 4707 6095 1239 Size: 1167 232 1349 346 374 1388 2579 Fragments arranged by size: 2579 1388 1349 1167 374 346 232 Bpu10I CC'TnA_GC Cuts at: 2423 3501 3590 2423 Size: 1078 89 6268 Fragments arranged by size: 6268 1078 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3654 5560 6425 6723 3654 Size: 1906 865 298 4366 Fragments arranged by size: 4366 1906 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6098 882 Size: 617 4599 2219 Fragments arranged by size: 4599 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5226 493 Size: 711 4022 2702 Fragments arranged by size: 4022 2702 711 BsaBI GATnn'nnATC Cuts at: 7429 7429 Size: 7435 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5242 5624 6259 275 Size: 53 83 186 936 3709 382 635 1451 Fragments arranged by size: 3709 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4056 5868 6266 6540 6687 7098 4056 Size: 1812 398 274 147 411 4393 Fragments arranged by size: 4393 1812 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3689 3719 1178 Size: 30 165 30 2286 30 4894 Fragments arranged by size: 4894 2286 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4373 1186 Size: 3187 4248 BseYI C'CCAG_C Cuts at: 2633 4086 6638 7134 2633 Size: 1453 2552 496 2934 Fragments arranged by size: 2934 2552 1453 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3958 4239 1163 Size: 573 2222 281 4359 Fragments arranged by size: 4359 2222 573 281 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4885 5646 6674 1540 Size: 275 186 2884 761 1028 2301 Fragments arranged by size: 2884 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2392 2907 5496 5581 6652 2392 Size: 515 2589 85 1071 3175 Fragments arranged by size: 3175 2589 1071 515 85 BsmI GAATG_Cn' Cuts at: 4487 4946 5039 4487 Size: 459 93 6883 Fragments arranged by size: 6883 459 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2392 2468 2907 4133 4410 4754 5496 Size: 399 602 76 439 1226 277 344 742 Cuts at: 5496 5581 6652 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1226 1071 742 602 439 399 344 277 85 76 BspEI T'CCGG_A Cuts at: 7098 7098 Size: 7435 BspHI T'CATG_A Cuts at: 5324 7054 5324 Size: 1730 5705 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2872 4790 844 Size: 2028 1918 3489 Fragments arranged by size: 3489 2028 1918 BsrBI CCG'CTC Cuts at: 1394 1823 5322 1394 Size: 429 3499 3507 Fragments arranged by size: 3507 3499 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2959 4155 5930 6104 65 Size: 1183 1711 1196 1775 174 1396 Fragments arranged by size: 1775 1711 1396 1196 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6079 6266 1700 Size: 110 4269 187 2869 Fragments arranged by size: 4269 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7435 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7435 BssSI C'ACGA_G Cuts at: 4067 5495 6507 4067 Size: 1428 1012 4995 Fragments arranged by size: 4995 1428 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7435 BstBI TT'CG_AA Cuts at: 2614 6239 2614 Size: 3625 3810 BstEII G'GTnAC_C Cuts at: 1184 2164 2602 1184 Size: 980 438 6017 Fragments arranged by size: 6017 980 438 BstXI CCAn_nnnn'nTGG Cuts at: 2235 2235 Size: 7435 BstYI r'GATC_y Cuts at: 1972 3422 4765 5132 5517 5534 6986 7072 7084 Size: 1450 1343 367 385 17 1452 86 12 Cuts at: 7084 7430 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1450 1343 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2056 3525 3951 513 Size: 552 991 1469 426 3997 Fragments arranged by size: 3997 1469 991 552 426 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6696 Fragments arranged by size: 6696 683 56 BtsI GCAGTG_nn' Cuts at: 2315 2665 2839 3638 4649 4951 5745 5765 2315 Size: 350 174 799 1011 302 794 20 3985 Fragments arranged by size: 3985 1011 799 794 350 302 174 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7435 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3009 3044 529 Size: 35 2445 35 4920 Fragments arranged by size: 4920 2445 35 35 DraI TTT'AAA Cuts at: 4739 4739 Size: 7435 DraIII CAC_nnn'GTG Cuts at: 3658 3658 Size: 7435 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6442 809 Size: 507 5126 1802 Fragments arranged by size: 5126 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 3084 4882 Size: 54 1425 50 275 18 223 1031 1798 Cuts at: 4882 5771 7400 8 Size: 889 1629 43 Fragments arranged by size: 1798 1629 1425 1031 889 275 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4882 1537 Size: 275 3070 4090 Fragments arranged by size: 4090 3070 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3267 4370 5365 1353 Size: 1914 1103 995 3423 Fragments arranged by size: 3423 1914 1103 995 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6022 6396 6542 387 Size: 1064 138 4433 374 146 1280 Fragments arranged by size: 4433 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2231 2406 2638 3987 4333 4707 Size: 393 512 87 175 232 1349 346 374 Cuts at: 4707 5498 6095 6882 7150 1239 Size: 791 597 787 268 1524 Fragments arranged by size: 1524 1349 791 787 597 512 393 374 346 268 232 175 87 EcoNI CCTnn'n_nnAGG Cuts at: 4186 4401 4186 Size: 215 7220 EcoRI G'AATT_C Cuts at: 2506 2577 4744 5122 2506 Size: 71 2167 378 4819 Fragments arranged by size: 4819 2167 378 71 EcoRV GAT'ATC Cuts at: 3522 3522 Size: 7435 FspI TGC'GCA Cuts at: 5941 5941 Size: 7435 HaeII r_GCGC'y Cuts at: 1678 6582 1678 Size: 4904 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3771 3803 4488 4520 6076 Size: 32 135 32 2199 32 685 32 1556 Cuts at: 6076 6108 6150 6182 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2199 1556 685 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3459 4779 5025 669 Size: 988 281 1521 1320 246 3079 Fragments arranged by size: 3079 1521 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7435 HpaI GTT'AAC Cuts at: 5025 5025 Size: 7435 KpnI G_GTAC'C Cuts at: 4760 4760 Size: 7435 MfeI C'AATT_G Cuts at: 5034 5034 Size: 7435 MluI A'CGCG_T Cuts at: 7311 7311 Size: 7435 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1993 3278 4087 4722 6549 6733 1305 Size: 688 1285 809 635 1827 184 2007 Fragments arranged by size: 2007 1827 1285 809 688 635 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3086 10 Size: 54 1425 343 1254 4359 Fragments arranged by size: 4359 1425 1254 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2233 3245 3719 3773 3953 4067 4367 Size: 648 1067 1012 474 54 180 114 300 Cuts at: 4367 5393 5752 5911 518 Size: 1026 359 159 2042 Fragments arranged by size: 2042 1067 1026 1012 648 474 359 300 180 159 114 54 MspA1I CmG'CkG Cuts at: 1606 2152 2969 3980 4851 5528 6676 6921 1606 Size: 546 817 1011 871 677 1148 245 2120 Fragments arranged by size: 2120 1148 1011 871 817 677 546 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6883 NdeI CA'TA_TG Cuts at: 387 2569 387 Size: 2182 5253 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7435 NotI GC'GGCC_GC Cuts at: 4882 4882 Size: 7435 NsiI A_TGCA'T Cuts at: 4489 4675 4489 Size: 186 7249 NspI r_CATG'y Cuts at: 1132 1229 2693 4466 4793 6338 1132 Size: 97 1464 1773 327 1545 2229 Fragments arranged by size: 2229 1773 1545 1464 327 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7435 PciI A'CATG_T Cuts at: 2689 6334 2689 Size: 3645 3790 PflMI CCAn_nnn'nTGG Cuts at: 1108 4081 4453 1108 Size: 2973 372 4090 Fragments arranged by size: 4090 2973 372 PmeI GTTT'AAAC Cuts at: 4739 4739 Size: 7435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5504 5536 7017 7049 5504 Size: 32 1481 32 5890 Fragments arranged by size: 5890 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7435 PsiI TTA'TAA Cuts at: 5005 5005 Size: 7435 PstI C_TGCA'G Cuts at: 830 2310 2831 4787 830 Size: 1480 521 1956 3478 Fragments arranged by size: 3478 1956 1480 521 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7435 PvuII CAG'CTG Cuts at: 2152 2969 3980 2152 Size: 817 1011 5607 Fragments arranged by size: 5607 1011 817 SalI G'TCGA_C Cuts at: 1936 4777 1936 Size: 2841 4594 SapI GCTCTTCn'nnn_ Cuts at: 3267 4370 3267 Size: 1103 6332 SbfI CC_TGCA'GG Cuts at: 4787 4787 Size: 7435 ScaI AGT'ACT Cuts at: 5683 7395 5683 Size: 1712 5723 SexAI A'CCwGG_T Cuts at: 2333 2333 Size: 7435 SfcI C'TryA_G Cuts at: 826 1045 2170 2306 2827 2839 4783 5918 6599 Size: 219 1125 136 521 12 1944 1135 681 Cuts at: 6599 6790 826 Size: 191 1471 Fragments arranged by size: 1944 1471 1135 1125 681 521 219 191 136 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7435 SmaI CCC'GGG Cuts at: 1872 4762 1872 Size: 2890 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3669 4570 5539 6440 6702 820 Size: 197 926 1726 901 969 901 262 1553 Fragments arranged by size: 1726 1553 969 926 901 901 262 197 SnaBI TAC'GTA Cuts at: 493 5226 493 Size: 4733 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7435 SphI G_CATG'C Cuts at: 1132 4793 1132 Size: 3661 3774 SspI AAT'ATT Cuts at: 5 52 3067 5359 5 Size: 47 3015 2292 2081 Fragments arranged by size: 3015 2292 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7435 StyI C'CwwG_G Cuts at: 513 1065 2181 2802 4874 513 Size: 552 1116 621 2072 3074 Fragments arranged by size: 3074 2072 1116 621 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2621 2865 4116 5462 5479 5632 5817 Size: 1255 1041 244 1251 1346 17 153 185 Cuts at: 5817 6262 325 Size: 445 1498 Fragments arranged by size: 1498 1346 1255 1251 1041 445 244 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3692 5681 7393 Size: 274 80 33 51 1443 1715 1989 1712 Cuts at: 7393 96 Size: 138 Fragments arranged by size: 1989 1715 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2323 2355 3689 3717 3721 3749 4422 4454 7395 Size: 32 1334 28 4 28 673 32 2941 Cuts at: 7395 7427 2323 Size: 32 2331 Fragments arranged by size: 2941 2331 1334 673 32 32 32 28 28 4 Tth111I GACn'n_nGTC Cuts at: 1316 2783 4338 1316 Size: 1467 1555 4413 Fragments arranged by size: 4413 1555 1467 XbaI T'CTAG_A Cuts at: 4771 4771 Size: 7435 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2615 3112 4366 2615 Size: 497 1254 5684 Fragments arranged by size: 5684 1254 497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7435 XmaI C'CCGG_G Cuts at: 1870 4760 1870 Size: 2890 4545 XmnI GAAnn'nnTTC Cuts at: 2577 5564 2577 Size: 2987 4448 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5243 276 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI