(Linear) (Six-base) MAPSORT of: KIEE9453.seq Check: 6189 from: 1 to: 2730 KIEE9453.orf With 182 enzymes: SgfI * January 29, 2014 12:07 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 226 2730 Size: 139 87 2504 Fragments arranged by size: 2504 139 87 AflII C'TTAA_G Cuts at: 0 2565 2730 Size: 2565 165 AflIII A'CryG_T Cuts at: 0 684 1864 2730 Size: 684 1180 866 Fragments arranged by size: 1180 866 684 AleI CACnn'nnGTG Cuts at: 0 1240 1768 1948 2730 Size: 1240 528 180 782 Fragments arranged by size: 1240 782 528 180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1258 2730 Size: 1258 1472 AlwNI CAG_nnn'CTG Cuts at: 0 150 1978 2448 2730 Size: 150 1828 470 282 Fragments arranged by size: 1828 470 282 150 ApoI r'AATT_y Cuts at: 0 198 501 545 572 927 1266 1279 2623 Size: 198 303 44 27 355 339 13 1344 Cuts at: 2623 2730 Size: 107 Fragments arranged by size: 1344 355 339 303 198 107 44 27 13 AseI AT'TA_AT Cuts at: 0 473 2730 Size: 473 2257 BanI G'GyrC_C Cuts at: 0 1242 1930 2123 2730 Size: 1242 688 193 607 Fragments arranged by size: 1242 688 607 193 BanII G_rGCy'C Cuts at: 0 2405 2730 Size: 2405 325 BbsI GAAGACnn'nnnn_ Cuts at: 0 323 740 2730 Size: 323 417 1990 Fragments arranged by size: 1990 417 323 BbvCI CC'TCA_GC Cuts at: 0 418 2730 Size: 418 2312 BclI T'GATC_A Cuts at: 0 1811 2730 Size: 1811 919 BfrBI ATG'CAT Cuts at: 0 2482 2668 2730 Size: 2482 186 62 Fragments arranged by size: 2482 186 62 BglI GCCn_nnn'nGGC Cuts at: 0 56 697 958 2730 Size: 56 641 261 1772 Fragments arranged by size: 1772 641 261 56 Bme1580I G_kGCm'C Cuts at: 0 463 2128 2730 Size: 463 1665 602 Fragments arranged by size: 1665 602 463 BmgBI CAC'GTC Cuts at: 0 1679 1867 2730 Size: 1679 188 863 Fragments arranged by size: 1679 863 188 BmrI ACTGGGnnnn_n' Cuts at: 0 124 759 1786 2730 Size: 124 635 1027 944 Fragments arranged by size: 1027 944 635 124 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1610 2730 Size: 1610 1120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 401 633 1982 2328 2702 2730 Size: 401 232 1349 346 374 28 Fragments arranged by size: 1349 401 374 346 232 28 Bpu10I CC'TnA_GC Cuts at: 0 418 1496 1585 2730 Size: 418 1078 89 1145 Fragments arranged by size: 1145 1078 418 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1649 2730 Size: 1649 1081 BsaWI w'CCGG_w Cuts at: 0 2051 2730 Size: 2051 679 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1684 1714 2730 Size: 1684 30 1016 Fragments arranged by size: 1684 1016 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2368 2730 Size: 2368 362 BseYI C'CCAG_C Cuts at: 0 628 2081 2730 Size: 628 1453 649 Fragments arranged by size: 1453 649 628 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1953 2234 2730 Size: 1953 281 496 Fragments arranged by size: 1953 496 281 BsiHKAI G_wGCw'C Cuts at: 0 387 902 2730 Size: 387 515 1828 Fragments arranged by size: 1828 515 387 BsmI GAATG_Cn' Cuts at: 0 2482 2730 Size: 2482 248 Bsp1286I G_dGCh'C Cuts at: 0 387 463 902 2128 2405 2730 Size: 387 76 439 1226 277 325 Fragments arranged by size: 1226 439 387 325 277 76 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 867 2730 Size: 867 1863 BsrDI GCAATG_nn' Cuts at: 0 954 2150 2730 Size: 954 1196 580 Fragments arranged by size: 1196 954 580 BssSI C'ACGA_G Cuts at: 0 2062 2730 Size: 2062 668 BstBI TT'CG_AA Cuts at: 0 609 2730 Size: 609 2121 BstEII G'GTnAC_C Cuts at: 0 159 597 2730 Size: 159 438 2133 Fragments arranged by size: 2133 438 159 BstXI CCAn_nnnn'nTGG Cuts at: 0 230 2730 Size: 230 2500 BstYI r'GATC_y Cuts at: 0 1417 2730 Size: 1417 1313 BtgI C'CryG_G Cuts at: 0 51 1520 1946 2730 Size: 51 1469 426 784 Fragments arranged by size: 1469 784 426 51 BtsI GCAGTG_nn' Cuts at: 0 310 660 834 1633 2644 2730 Size: 310 350 174 799 1011 86 Fragments arranged by size: 1011 799 350 310 174 86 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1004 1039 2730 Size: 1004 35 1691 Fragments arranged by size: 1691 1004 35 DraIII CAC_nnn'GTG Cuts at: 0 1653 2730 Size: 1653 1077 EaeI y'GGCC_r Cuts at: 0 48 1079 2730 Size: 48 1031 1651 Fragments arranged by size: 1651 1031 48 EarI CTCTTCn'nnn_ Cuts at: 0 1262 2365 2730 Size: 1262 1103 365 Fragments arranged by size: 1262 1103 365 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 226 401 633 1982 2328 2702 2730 Size: 139 87 175 232 1349 346 374 28 Fragments arranged by size: 1349 374 346 232 175 139 87 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 2181 2396 2730 Size: 2181 215 334 Fragments arranged by size: 2181 334 215 EcoRI G'AATT_C Cuts at: 0 501 572 2730 Size: 501 71 2158 Fragments arranged by size: 2158 501 71 EcoRV GAT'ATC Cuts at: 0 1517 2730 Size: 1517 1213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1766 1798 2483 2515 2730 Size: 1766 32 685 32 215 Fragments arranged by size: 1766 685 215 32 32 HincII GTy'rAC Cuts at: 0 1454 2730 Size: 1454 1276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1273 2082 2717 2730 Size: 1273 809 635 13 Fragments arranged by size: 1273 809 635 13 MscI TGG'CCA Cuts at: 0 1081 2730 Size: 1081 1649 MslI CAynn'nnrTG Cuts at: 0 228 1240 1714 1768 1948 2062 2362 2730 Size: 228 1012 474 54 180 114 300 368 Fragments arranged by size: 1012 474 368 300 228 180 114 54 MspA1I CmG'CkG Cuts at: 0 147 964 1975 2730 Size: 147 817 1011 755 Fragments arranged by size: 1011 817 755 147 NdeI CA'TA_TG Cuts at: 0 564 2730 Size: 564 2166 NsiI A_TGCA'T Cuts at: 0 2484 2670 2730 Size: 2484 186 60 Fragments arranged by size: 2484 186 60 NspI r_CATG'y Cuts at: 0 688 2461 2730 Size: 688 1773 269 Fragments arranged by size: 1773 688 269 PciI A'CATG_T Cuts at: 0 684 2730 Size: 684 2046 PflMI CCAn_nnn'nTGG Cuts at: 0 2076 2448 2730 Size: 2076 372 282 Fragments arranged by size: 2076 372 282 PstI C_TGCA'G Cuts at: 0 305 826 2730 Size: 305 521 1904 Fragments arranged by size: 1904 521 305 PvuII CAG'CTG Cuts at: 0 147 964 1975 2730 Size: 147 817 1011 755 Fragments arranged by size: 1011 817 755 147 SapI GCTCTTCn'nnn_ Cuts at: 0 1262 2365 2730 Size: 1262 1103 365 Fragments arranged by size: 1262 1103 365 SexAI A'CCwGG_T Cuts at: 0 328 2730 Size: 328 2402 SfcI C'TryA_G Cuts at: 0 165 301 822 834 2730 Size: 165 136 521 12 1896 Fragments arranged by size: 1896 521 165 136 12 SmlI C'TyrA_G Cuts at: 0 1664 2565 2730 Size: 1664 901 165 Fragments arranged by size: 1664 901 165 SspI AAT'ATT Cuts at: 0 1062 2730 Size: 1062 1668 StyI C'CwwG_G Cuts at: 0 176 797 2730 Size: 176 621 1933 Fragments arranged by size: 1933 621 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 616 860 2111 2730 Size: 616 244 1251 619 Fragments arranged by size: 1251 619 616 244 TatI w'GTAC_w Cuts at: 0 1687 2730 Size: 1687 1043 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 318 350 1684 1712 1716 1744 2417 2449 Size: 318 32 1334 28 4 28 673 32 Cuts at: 2449 2730 Size: 281 Fragments arranged by size: 1334 673 318 281 32 32 28 28 4 Tth111I GACn'n_nGTC Cuts at: 0 778 2333 2730 Size: 778 1555 397 Fragments arranged by size: 1555 778 397 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 610 1107 2361 2730 Size: 610 497 1254 369 Fragments arranged by size: 1254 610 497 369 XmnI GAAnn'nnTTC Cuts at: 0 572 2730 Size: 572 2158 Enzymes that do cut: AcuI AflII AflIII AleI AlfI AlwNI ApoI AseI BanI BanII BbsI BbvCI BclI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstBI BstEII BstXI BstYI BtgI BtsI CspCI DraIII EaeI EarI Eco57MI EcoNI EcoRI EcoRV Hin4I HincII MmeI MscI MslI MspA1I NdeI NsiI NspI PciI PflMI PstI PvuII SapI SexAI SfcI SmlI SspI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI BglII BlpI BmtI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EciI EcoICRI EcoO109I FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XhoI XmaI ZraI