(Circular) (Six-base) MAPSORT of: pFN21AE6662.seq Check: 2491 from: 1 to: 5533 pFN21AE6662 5533 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2787 2876 1937 Size: 850 89 4594 Fragments arranged by size: 4594 850 89 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 3660 4033 1563 Size: 2097 373 3063 Fragments arranged by size: 3063 2097 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3596 4980 5248 1632 Size: 1964 1384 268 1917 Fragments arranged by size: 1964 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 4432 5409 1203 Size: 3229 977 1327 Fragments arranged by size: 3229 1327 977 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 4262 4262 Size: 5533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 2191 3276 4848 2191 Size: 1085 1572 2876 Fragments arranged by size: 2876 1572 1085 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2842 3068 3220 5329 1493 Size: 1349 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2469 2858 1870 Size: 73 526 389 4545 Fragments arranged by size: 4545 526 389 73 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2474 2854 4309 618 Size: 325 217 627 687 380 1455 1842 Fragments arranged by size: 1842 1455 687 627 380 325 217 BanII G_rGCy'C Cuts at: 1391 2852 1391 Size: 1461 4072 BbeI G_GCGC'C Cuts at: 2478 2478 Size: 5533 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4562 BbvCI CC'TCA_GC Cuts at: 2063 2063 Size: 5533 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3724 3758 1903 Size: 34 1787 34 3678 Fragments arranged by size: 3678 1787 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4144 136 Size: 107 122 71 631 605 2472 1525 Fragments arranged by size: 2472 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5528 5528 Size: 5533 BlpI GC'TnA_GC Cuts at: 2555 2950 2555 Size: 395 5138 Bme1580I G_kGCm'C Cuts at: 1790 2798 3594 4750 1790 Size: 1008 796 1156 2573 Fragments arranged by size: 2573 1156 1008 796 BmgBI CAC'GTC Cuts at: 1318 2094 1318 Size: 776 4757 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3264 4222 442 Size: 416 53 83 399 24 1847 958 1753 Fragments arranged by size: 1847 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2495 2495 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2701 4193 1239 Size: 815 647 1492 2579 Fragments arranged by size: 2579 1492 815 647 Bpu10I CC'TnA_GC Cuts at: 2063 2063 Size: 5533 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2630 3658 4523 4821 2630 Size: 1028 865 298 3342 Fragments arranged by size: 3342 1028 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2408 4196 882 Size: 617 909 1788 2219 Fragments arranged by size: 2219 1788 909 617 BsaAI yAC'GTr Cuts at: 493 1204 2791 3324 493 Size: 711 1587 533 2702 Fragments arranged by size: 2702 1587 711 533 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2042 2475 3340 Size: 53 83 186 936 477 32 433 865 Cuts at: 3340 3722 4357 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 865 635 477 433 382 186 83 53 32 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 1178 Size: 30 165 30 624 30 4654 Fragments arranged by size: 4654 624 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 1186 Size: 1102 4431 BseYI C'CCAG_C Cuts at: 4736 5232 4736 Size: 496 5037 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2224 1163 Size: 573 488 4472 Fragments arranged by size: 4472 573 488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2983 3744 4772 1540 Size: 275 186 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3594 3679 4750 3594 Size: 85 1071 4377 Fragments arranged by size: 4377 1071 85 BsmI GAATG_Cn' Cuts at: 3044 3137 3044 Size: 93 5440 BsmBI CGTCTCn'nnnn_ Cuts at: 2100 2100 Size: 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2798 2852 3594 3679 4750 1391 Size: 399 1008 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 1008 742 399 85 54 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 3422 5152 3422 Size: 1730 3803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2888 844 Size: 2044 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2710 4028 4202 65 Size: 1183 1462 1318 174 1396 Fragments arranged by size: 1462 1396 1318 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4177 4364 1700 Size: 110 2367 187 2869 Fragments arranged by size: 2869 2367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5533 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528 Size: 891 367 385 17 1452 86 12 346 Cuts at: 5528 1972 Size: 1977 Fragments arranged by size: 1977 1452 891 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4042 Fragments arranged by size: 4042 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2164 487 Size: 683 56 938 3856 Fragments arranged by size: 3856 938 683 56 BtsI GCAGTG_nn' Cuts at: 2056 3049 3843 3863 2056 Size: 993 794 20 3726 Fragments arranged by size: 3726 993 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2837 2837 Size: 5533 DraIII CAC_nnn'GTG Cuts at: 2247 2247 Size: 5533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4540 809 Size: 507 3224 1802 Fragments arranged by size: 3224 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2457 2735 2980 Size: 54 1425 50 275 18 627 278 245 Cuts at: 2980 3869 5498 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 627 278 275 245 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2980 1537 Size: 275 1168 4090 Fragments arranged by size: 4090 1168 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 2406 2486 3463 1353 Size: 791 262 80 977 3423 Fragments arranged by size: 3423 977 791 262 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4120 4494 4640 387 Size: 1064 138 2531 374 146 1280 Fragments arranged by size: 2531 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2701 3596 4193 4980 5248 1239 Size: 393 422 647 895 597 787 268 1524 Fragments arranged by size: 1524 895 787 647 597 422 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2195 2195 Size: 5533 EcoO109I rG'GnC_Cy Cuts at: 2059 2188 2320 2676 2059 Size: 129 132 356 4916 Fragments arranged by size: 4916 356 132 129 EcoRI G'AATT_C Cuts at: 2842 3220 2842 Size: 378 5155 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2326 2326 Size: 5533 FspI TGC'GCA Cuts at: 2723 4039 2723 Size: 1316 4217 HaeII r_GCGC'y Cuts at: 1678 2478 4680 1678 Size: 800 2202 2531 Fragments arranged by size: 2531 2202 800 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 4174 4206 4248 Size: 32 135 32 455 32 2115 32 42 Cuts at: 4248 4280 1373 Size: 32 2626 Fragments arranged by size: 2626 2115 455 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KasI G'GCGC_C Cuts at: 2474 2474 Size: 5533 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 5409 5409 Size: 5533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4647 4831 1305 Size: 3342 184 2007 Fragments arranged by size: 3342 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2459 10 Size: 54 1425 343 627 3084 Fragments arranged by size: 3084 1425 627 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 3491 3850 4009 518 Size: 648 1524 801 359 159 2042 Fragments arranged by size: 2042 1524 801 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2949 3626 4774 5019 1606 Size: 1343 677 1148 245 2120 Fragments arranged by size: 2120 1343 1148 677 245 NarI GG'CG_CC Cuts at: 2475 2475 Size: 5533 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4042 Fragments arranged by size: 4042 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2891 4436 1132 Size: 97 1662 1545 2229 Fragments arranged by size: 2229 1662 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5533 PciI A'CATG_T Cuts at: 4432 4432 Size: 5533 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5533 PfoI T'CCnGG_A Cuts at: 2678 2678 Size: 5533 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 2676 2320 Size: 356 5177 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 3103 3103 Size: 5533 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2765 2797 2765 Size: 32 5501 PstI C_TGCA'G Cuts at: 830 2355 2885 830 Size: 1525 530 3478 Fragments arranged by size: 3478 1525 530 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 RsrII CG'GwC_CG Cuts at: 2071 2071 Size: 5533 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SanDI GG'GwC_CC Cuts at: 2320 2320 Size: 5533 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SfcI C'TryA_G Cuts at: 826 1045 2227 2351 2881 4016 4697 4888 826 Size: 219 1182 124 530 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 681 530 219 191 124 SfoI GGC'GCC Cuts at: 2476 2476 Size: 5533 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2860 1872 Size: 988 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2609 3637 4538 4800 820 Size: 197 926 666 1028 901 262 1553 Fragments arranged by size: 1553 1028 926 901 666 262 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2004 2155 2652 2972 513 Size: 552 939 151 497 320 3074 Fragments arranged by size: 3074 939 552 497 320 151 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2519 3560 3577 3730 3915 4360 325 Size: 1255 939 1041 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1041 939 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3779 5491 96 Size: 274 80 33 51 1443 1802 1712 138 Fragments arranged by size: 1802 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5533 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2275 2275 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2858 1870 Size: 988 4545 XmnI GAAnn'nnTTC Cuts at: 3662 3662 Size: 5533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII SacI SacII SapI SexAI SfiI SrfI SwaI