################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MluNI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 414 TCCGGA T^CCGGA AcsI 854 AAATTC [GA]^AATT[CT] AflIII 376 ACACGT A^C[GA][CT]GT AgeI 550 ACCGGT A^CCGGT ApoI 854 AAATTC [GA]^AATT[CT] BanII 721 GAGCTC G[GA]GC[CT]^C BciVI 417 GGATAC GTATCC(6/5) BclI 705 TGATCA T^GATCA BfaI 142 CTAG C^TAG BseAI 414 TCCGGA T^CCGGA BsiEI 1008 CGGCCG CG[GA][CT]^CG BsiYI 829 CCACCAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 829 CCACCAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 146 GTCCC GGGAC(10/14) Bsp1407I 1035 TGTACA T^GTACA BspEI 414 TCCGGA T^CCGGA BsrGI 1035 TGTACA T^GTACA BstBI 1109 TTCGAA TT^CGAA BstEII 967 GGTTACC G^GT[ACGT]ACC BstPI 967 GGTTACC G^GT[ACGT]ACC BstXI 734 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 12 CCGTGG C^C[GA][CT]GG EaeI 1008 CGGCCG [CT]^GGCC[GA] EagI 1008 CGGCCG C^GGCCG EclXI 1008 CGGCCG C^GGCCG Eco52I 1008 CGGCCG C^GGCCG EcoO65I 967 GGTTACC G^GT[ACGT]ACC EcoT22I 1177 ATGCAT ATGCA^T FbaI 705 TGATCA T^GATCA HgaI 357 GACGC GACGC(5/10) MaeI 142 CTAG C^TAG MaeII 378 ACGT A^CGT MfeI 561 CAATTG C^AATTG MroI 414 TCCGGA T^CCGGA MspA1I 51 CAGCTG C[AC]G^C[GT]G MunI 561 CAATTG C^AATTG MwoI 723 GCTCATCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NsiI 1177 ATGCAT ATGCA^T NspV 1109 TTCGAA TT^CGAA PinAI 550 ACCGGT A^CCGGT PleI 1083 GAGTC GAGTC(4/5) PpuMI 144 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 51 CAGCTG CAG^CTG SacI 721 GAGCTC GAGCT^C SapI 718 GAAGAGC GCTCTTC(1/4) SfuI 1109 TTCGAA TT^CGAA SspBI 1035 TGTACA T^GTACA SspI 845 AATATT AAT^ATT StuI 1000 AGGCCT AGG^CCT XcmI 50 CCAGCTGCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 39 GCGG CCGC(-3/-1) 1007 GCGG AfaI 456 GTAC GT^AC 1036 GTAC AseI 619 ATTAAT AT^TAAT 1173 ATTAAT AsnI 619 ATTAAT AT^TAAT 1173 ATTAAT AspHI 689 GTGCTC G[AT]GC[AT]^C 721 GAGCTC BbvI 53 GCTGC GCAGC(8/12) 1138 GCTGC BglII 588 AGATCT A^GATCT 651 AGATCT BmyI 689 GTGCTC G[AGT]GC[ACT]^C 721 GAGCTC BpmI 287 CTCCAG CTGGAG(16/14) 1142 CTGGAG BsaJI 12 CCGTGG C^C[ACGT][ACGT]GG 148 CCCAGG BsaWI 414 TCCGGA [AT]^CCGG[AT] 550 ACCGGT BsiHKAI 689 GTGCTC G[AT]GC[AT]^C 721 GAGCTC Bsp1286I 689 GTGCTC G[AGT]GC[ACT]^C 721 GAGCTC BsrI 809 ACTGG ACTGG(1/-1) 1101 CCAGT BstYI 588 AGATCT [GA]^GATC[CT] 651 AGATCT Cac8I 49 GCCAGC GC[ACGT]^[ACGT]GC 186 GCCAGC DraII 144 AGGTCCC [GA]G^G[ACGT]CC[CT] 935 GGGGCCT EcoO109I 144 AGGTCCC [GA]G^G[ACGT]CC[CT] 935 GGGGCCT HphI 9 TCACC GGTGA(8/7) 1182 TCACC MflI 588 AGATCT [GA]^GATC[CT] 651 AGATCT PshBI 619 ATTAAT AT^TAAT 1173 ATTAAT RsaI 456 GTAC GT^AC 1036 GTAC SfcI 77 CTGTAG C^T[GA][CT]AG 876 CTACAG TaqI 716 TCGA T^CGA 1110 TCGA TseI 53 GCTGC G^C[AT]GC 1138 GCTGC TthHB8I 716 TCGA T^CGA 1110 TCGA XhoII 588 AGATCT [GA]^GATC[CT] 651 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AluI AvaII BbsI BpuAI BsrFI BssKI BstF5I BstNI Cfr10I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I Tsp45I Tsp509I TspRI