################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 27 CCGC CCGC(-3/-1) AfaI 408 GTAC GT^AC AluI 324 AGCT AG^CT Alw44I 349 GTGCAC G^TGCAC AlwNI 440 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 349 GTGCAC G^TGCAC AspHI 349 GTGCAC G[AT]GC[AT]^C AvaI 44 CCCGAG C^[CT]CG[GA]G AvaII 318 GGTCC G^G[AT]CC BanII 126 GGGCTC G[GA]GC[CT]^C BbsI 274 GAAGAC GAAGAC(2/6) BcgI 369 CGAGGTAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 124 CCGGG CC^[GC]GG BfaI 208 CTAG C^TAG BpuAI 274 GAAGAC GAAGAC(2/6) BsaI 132 GGTCTC GGTCTC(1/5) BsiHKAI 349 GTGCAC G[AT]GC[AT]^C BsiYI 411 CCTGCAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 411 CCTGCAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 133 GTCTC GTCTC(1/5) BsmFI 319 GTCCC GGGAC(10/14) BsoBI 44 CCCGAG C^[CT]CG[GA]G BspMI 410 ACCTGC ACCTGC(4/8) BsrDI 380 CATTGC GCAATG(2/0) BstAPI 414 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 185 CCAGGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 233 CTCAG C^T[ACGT]AG EaeI 365 TGGCCG [CT]^GGCC[GA] FauI 26 CCCGC CCCGC(4/6) HgaI 218 GCGTC GACGC(5/10) HincII 220 GTCAAC GT[CT]^[GA]AC HindII 220 GTCAAC GT[CT]^[GA]AC MaeI 208 CTAG C^TAG MaeII 119 ACGT A^CGT MseI 299 TTAA T^TAA NciI 124 CCGGG CC^[GC]GG NcoI 59 CCATGG C^CATGG NlaIII 60 CATG CATG^ NlaIV 318 GGTCCC GG[ACGT]^[ACGT]CC RsaI 408 GTAC GT^AC TaqI 269 TCGA T^CGA TfiI 103 GATTC G^A[AT]TC Tru9I 299 TTAA T^TAA Tsp45I 430 GTCAC ^GT[GC]AC Tsp509I 375 AATT ^AATT TthHB8I 269 TCGA T^CGA XcmI 185 CCAGGGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BmyI 126 GGGCTC G[AGT]GC[ACT]^C 349 GTGCAC Bsp1286I 126 GGGCTC G[AGT]GC[ACT]^C 349 GTGCAC BsrFI 3 GCCGGC [GA]^CCGG[CT] 93 GCCGGC BstF5I 228 CATCC GGATG(2/0) 242 CATCC BstNI 185 CCAGG CC^[AT]GG 426 CCAGG Cfr10I 3 GCCGGC [GA]^CCGG[CT] 93 GCCGGC Cfr13I 188 GGGCC G^G[ACGT]CC 318 GGTCC DpnII 180 GATC ^GATC 267 GATC DsaI 59 CCATGG C^C[GA][CT]GG 191 CCGTGG EcoRII 185 CCAGG ^CC[AT]GG 426 CCAGG FokI 228 CATCC GGATG(9/13) 242 CATCC HaeII 6 GGCGCT [GA]GCGC^[CT] 203 AGCGCC MaeIII 148 GTTAC ^GT[ACGT]AC 430 GTCAC MboI 180 GATC ^GATC 267 GATC MboII 264 GAAGA GAAGA(8/7) 274 GAAGA MvaI 185 CCAGG CC^[AT]GG 426 CCAGG MwoI 3 GCCGGCGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 414 GCAGTGGGTGC NaeI 3 GCCGGC GCC^GGC 93 GCCGGC NdeII 180 GATC ^GATC 267 GATC NgoMIV 3 GCCGGC G^CCGGC 93 GCCGGC PstI 137 CTGCAG CTGCA^G 412 CTGCAG Sau3AI 180 GATC ^GATC 267 GATC Sau96I 188 GGGCC G^G[ACGT]CC 318 GGTCC SfcI 137 CTGCAG C^T[GA][CT]AG 412 CTGCAG TspRI 279 CAGTG CA[GC]TG[ACGT][ACGT]^ 415 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### BbvI BsaJI BssKI Cac8I CfoI EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MnlI MspI PleI ScrFI StyI TseI