(Circular) (Six-base) MAPSORT of: pFN21AE6558.seq Check: 8706 from: 1 to: 5173 pFN21AE6558 5173 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2983 278 Size: 53 83 186 936 1447 2468 Fragments arranged by size: 2468 1447 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2516 1937 Size: 579 4594 Acc65I G'GTAC_C Cuts at: 2494 2494 Size: 5173 AclI AA'CG_TT Cuts at: 1563 3300 3673 1563 Size: 1737 373 3063 Fragments arranged by size: 3063 1737 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3236 4620 4888 1632 Size: 1604 1384 268 1917 Fragments arranged by size: 1917 1604 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4976 AflIII A'CryG_T Cuts at: 1203 4072 5049 1203 Size: 2869 977 1327 Fragments arranged by size: 2869 1327 977 AgeI A'CCGG_T Cuts at: 4004 4004 Size: 5173 AhdI GACnn_n'nnGTC Cuts at: 3902 3902 Size: 5173 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5173 AlwNI CAG_nnn'CTG Cuts at: 2450 2916 4488 2450 Size: 466 1572 3135 Fragments arranged by size: 3135 1572 466 ApaLI G'TGCA_C Cuts at: 2354 3230 4386 2354 Size: 876 1156 3141 Fragments arranged by size: 3141 1156 876 ApoI r'AATT_y Cuts at: 1493 2482 2708 2860 4969 1493 Size: 989 226 152 2109 1697 Fragments arranged by size: 2109 1697 989 226 152 AseI AT'TA_AT Cuts at: 160 1032 3727 160 Size: 872 2695 1606 Fragments arranged by size: 2695 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5173 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2498 1870 Size: 73 106 449 4545 Fragments arranged by size: 4545 449 106 73 BamHI G'GATC_C Cuts at: 2503 2870 2503 Size: 367 4806 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2494 3949 618 Size: 325 217 627 707 1455 1842 Fragments arranged by size: 1842 1455 707 627 325 217 BanII G_rGCy'C Cuts at: 1391 2135 2492 1391 Size: 744 357 4072 Fragments arranged by size: 4072 744 357 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2286 928 Size: 971 387 3815 Fragments arranged by size: 3815 971 387 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2363 2397 3364 3398 1903 Size: 34 50 34 342 34 967 34 3678 Fragments arranged by size: 3678 967 342 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3065 4275 3065 Size: 1210 3963 BclI T'GATC_A Cuts at: 1548 1548 Size: 5173 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3784 136 Size: 107 122 71 631 605 337 1775 1525 Fragments arranged by size: 1775 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5168 5168 Size: 5173 BlpI GC'TnA_GC Cuts at: 2590 2590 Size: 5173 Bme1580I G_kGCm'C Cuts at: 1790 2358 3234 4390 1790 Size: 568 876 1156 2573 Fragments arranged by size: 2573 1156 876 568 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5173 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2904 3862 442 Size: 416 53 83 399 24 1487 958 1753 Fragments arranged by size: 1753 1487 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5173 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3833 1239 Size: 2594 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3298 4163 4461 3298 Size: 865 298 4010 Fragments arranged by size: 4010 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2143 3836 882 Size: 617 644 1693 2219 Fragments arranged by size: 2219 1693 644 617 BsaAI yAC'GTr Cuts at: 493 1204 2964 493 Size: 711 1760 2702 Fragments arranged by size: 2702 1760 711 BsaBI GATnn'nnATC Cuts at: 5167 5167 Size: 5173 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2980 3362 3997 275 Size: 53 83 186 936 1447 382 635 1451 Fragments arranged by size: 1451 1447 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3606 4004 4278 4425 4836 3606 Size: 398 274 147 411 3943 Fragments arranged by size: 3943 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4948 Fragments arranged by size: 4948 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5173 BseYI C'CCAG_C Cuts at: 2088 2326 4376 4872 2088 Size: 238 2050 496 2389 Fragments arranged by size: 2389 2050 496 238 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4600 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2623 3384 4412 1540 Size: 275 186 622 761 1028 2301 Fragments arranged by size: 2301 1028 761 622 275 186 BsiHKAI G_wGCw'C Cuts at: 2358 3234 3319 4390 2358 Size: 876 85 1071 3141 Fragments arranged by size: 3141 1071 876 85 BsmI GAATG_Cn' Cuts at: 2684 2777 2684 Size: 93 5080 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2358 2492 3234 3319 4390 1391 Size: 399 345 223 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 345 223 134 85 BspEI T'CCGG_A Cuts at: 4836 4836 Size: 5173 BspHI T'CATG_A Cuts at: 3062 4792 3062 Size: 1730 3443 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2424 2528 844 Size: 1580 104 3489 Fragments arranged by size: 3489 1580 104 BsrBI CCG'CTC Cuts at: 1394 1823 3060 1394 Size: 429 1237 3507 Fragments arranged by size: 3507 1237 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2384 3668 3842 65 Size: 1183 1136 1284 174 1396 Fragments arranged by size: 1396 1284 1183 1136 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 2098 3817 4004 1700 Size: 110 198 90 1719 187 2869 Fragments arranged by size: 2869 1719 198 187 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5173 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5173 BssSI C'ACGA_G Cuts at: 3233 4245 3233 Size: 1012 4161 BstAPI GCAn_nnn'nTGC Cuts at: 794 2425 794 Size: 1631 3542 BstBI TT'CG_AA Cuts at: 3977 3977 Size: 5173 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5173 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 5173 BstYI r'GATC_y Cuts at: 1972 2503 2870 3255 3272 4724 4810 4822 5168 Size: 531 367 385 17 1452 86 12 346 Cuts at: 5168 1972 Size: 1977 Fragments arranged by size: 1977 1452 531 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2064 2196 513 Size: 552 999 132 3490 Fragments arranged by size: 3490 999 552 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4434 Fragments arranged by size: 4434 683 56 BtsI GCAGTG_nn' Cuts at: 2426 2689 3483 3503 2426 Size: 263 794 20 4096 Fragments arranged by size: 4096 794 263 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5173 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5138 DraI TTT'AAA Cuts at: 2477 2477 Size: 5173 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4180 809 Size: 507 2864 1802 Fragments arranged by size: 2864 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 2620 3509 Size: 54 1425 50 275 18 540 250 889 Cuts at: 3509 5138 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 540 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2620 1537 Size: 275 808 4090 Fragments arranged by size: 4090 808 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3103 1353 Size: 1750 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3760 4134 4280 387 Size: 1064 138 2171 374 146 1280 Fragments arranged by size: 2171 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3236 3833 4620 4888 1239 Size: 393 1604 597 787 268 1524 Fragments arranged by size: 1604 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2482 2860 2482 Size: 378 4795 FspI TGC'GCA Cuts at: 3679 3679 Size: 5173 HaeII r_GCGC'y Cuts at: 1678 2015 2212 4320 1678 Size: 337 197 2108 2531 Fragments arranged by size: 2531 2108 337 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 3814 3846 3888 Size: 32 135 32 527 32 1683 32 42 Cuts at: 3888 3920 1373 Size: 32 2626 Fragments arranged by size: 2626 1683 527 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 2517 2763 669 Size: 988 281 289 290 246 3079 Fragments arranged by size: 3079 988 290 289 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5173 HpaI GTT'AAC Cuts at: 2763 2763 Size: 5173 KpnI G_GTAC'C Cuts at: 2498 2498 Size: 5173 MfeI C'AATT_G Cuts at: 2772 2772 Size: 5173 MluI A'CGCG_T Cuts at: 5049 5049 Size: 5173 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2108 4287 4471 1305 Size: 803 2179 184 2007 Fragments arranged by size: 2179 2007 803 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3351 Fragments arranged by size: 3351 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3131 3490 3649 518 Size: 648 1965 359 159 2042 Fragments arranged by size: 2042 1965 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2589 3266 4414 4659 1606 Size: 983 677 1148 245 2120 Fragments arranged by size: 2120 1148 983 677 245 NaeI GCC'GGC Cuts at: 2010 2100 2010 Size: 90 5083 NcoI C'CATG_G Cuts at: 513 1065 2064 513 Size: 552 999 3622 Fragments arranged by size: 3622 999 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5173 NgoMIV G'CCGG_C Cuts at: 2008 2098 2008 Size: 90 5083 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5173 NotI GC'GGCC_GC Cuts at: 2620 2620 Size: 5173 NspI r_CATG'y Cuts at: 1132 1229 2531 4076 1132 Size: 97 1302 1545 2229 Fragments arranged by size: 2229 1545 1302 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5173 PciI A'CATG_T Cuts at: 4072 4072 Size: 5173 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5173 PmeI GTTT'AAAC Cuts at: 2477 2477 Size: 5173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3242 3274 4755 4787 3242 Size: 32 1481 32 3628 Fragments arranged by size: 3628 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5173 PsiI TTA'TAA Cuts at: 2743 2743 Size: 5173 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2396 2428 2396 Size: 32 5141 PstI C_TGCA'G Cuts at: 830 2146 2421 2525 830 Size: 1316 275 104 3478 Fragments arranged by size: 3478 1316 275 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5173 SalI G'TCGA_C Cuts at: 1936 2515 1936 Size: 579 4594 SbfI CC_TGCA'GG Cuts at: 2525 2525 Size: 5173 ScaI AGT'ACT Cuts at: 3421 5133 3421 Size: 1712 3461 SfcI C'TryA_G Cuts at: 826 1045 2142 2417 2521 3656 4337 4528 826 Size: 219 1097 275 104 1135 681 191 1471 Fragments arranged by size: 1471 1135 1097 681 275 219 191 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5173 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5173 SmaI CCC'GGG Cuts at: 1872 2500 1872 Size: 628 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3277 4178 4440 820 Size: 197 926 1334 901 262 1553 Fragments arranged by size: 1553 1334 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2964 493 Size: 2471 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5173 SphI G_CATG'C Cuts at: 1132 2531 1132 Size: 1399 3774 SspI AAT'ATT Cuts at: 5 52 3097 5 Size: 47 3045 2081 Fragments arranged by size: 3045 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5173 StyI C'CwwG_G Cuts at: 513 1065 2064 2178 2319 2612 513 Size: 552 999 114 141 293 3074 Fragments arranged by size: 3074 999 552 293 141 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2125 2413 3200 3217 3370 3555 4000 Size: 1255 545 288 787 17 153 185 445 Cuts at: 4000 325 Size: 1498 Fragments arranged by size: 1498 1255 787 545 445 288 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3419 5131 96 Size: 274 80 33 51 1443 1442 1712 138 Fragments arranged by size: 1712 1443 1442 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5133 5165 5133 Size: 32 5141 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5173 XbaI T'CTAG_A Cuts at: 2509 2509 Size: 5173 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 2197 Size: 5173 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5173 XmaI C'CCGG_G Cuts at: 1870 2498 1870 Size: 628 4545 XmnI GAAnn'nnTTC Cuts at: 3302 3302 Size: 5173 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2981 276 Size: 53 83 186 936 1447 2468 Fragments arranged by size: 2468 1447 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI