################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII BbrPI BbsI BbvCI BclI BglII BpuAI BsaAI BsaBI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflII 2050 CTTAAG C^TTAAG Aor51HI 610 AGCGCT AGC^GCT Asp700I 769 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1822 CCTAGG C^CTAGG BalI 1726 TGGCCA TGG^CCA BamHI 1004 GGATCC G^GATCC BbeI 1674 GGCGCC GGCGC^C BcgI 690 CGATCCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2050 CTTAAG C^TTAAG BlnI 1822 CCTAGG C^CTAGG BlpI 1847 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1847 GCTCAGC GC^T[ACGT]AGC BsaI 824 GAGACC GGTCTC(1/5) BsaWI 632 TCCGGT [AT]^CCGG[AT] BsrBI 1845 CCGCTC CCGCTC(-3/-3) CelII 1847 GCTCAGC GC^T[ACGT]AGC Eco47III 610 AGCGCT AGC^GCT EcoRV 1459 GATATC GAT^ATC HgaI 2225 GCGTC GACGC(5/10) HincII 489 GTCAAC GT[CT]^[GA]AC HindII 489 GTCAAC GT[CT]^[GA]AC KasI 1674 GGCGCC G^GCGCC KspI 7 CCGCGG CCGC^GG MaeII 258 ACGT A^CGT MluNI 1726 TGGCCA TGG^CCA MscI 1726 TGGCCA TGG^CCA MslI 2001 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1025 GCCGGC GCC^GGC NarI 1674 GGCGCC GG^CGCC NcoI 2159 CCATGG C^CATGG NgoMIV 1025 GCCGGC G^CCGGC PaeR7I 1832 CTCGAG C^TCGAG PflMI 1288 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 7 CCGCGG CCGC^GG ScaI 1657 AGTACT AGT^ACT SfiI 97 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1674 GGCGCC GGC^GCC TfiI 433 GAATC G^A[AT]TC Van91I 1288 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1832 CTCGAG C^TCGAG XmnI 769 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 8 CGCG CG^CG 1009 CGCG AcyI 1674 GGCGCC G[GA]^CG[CT]C 2224 GGCGTC AocI 511 CCTGAGG CC^T[ACGT]AGG 2246 CCTCAGG ApaI 96 GGGCCC GGGCC^C 983 GGGCCC BsaHI 1674 GGCGCC G[GA]^CG[CT]C 2224 GGCGTC BssSI 33 CACGAG CACGAG(-5/-1) 1201 CTCGTG BstEII 951 GGTGACC G^GT[ACGT]ACC 1837 GGTCACC BstPI 951 GGTGACC G^GT[ACGT]ACC 1837 GGTCACC BstUI 8 CGCG CG^CG 1009 CGCG BstYI 1004 GGATCC [GA]^GATC[CT] 1921 GGATCT Bsu36I 511 CCTGAGG CC^T[ACGT]AGG 2246 CCTCAGG CfoI 611 GCGC GCG^C 1675 GCGC Eco81I 511 CCTGAGG CC^T[ACGT]AGG 2246 CCTCAGG EcoO65I 951 GGTGACC G^GT[ACGT]ACC 1837 GGTCACC HaeII 610 AGCGCT [GA]GCGC^[CT] 1674 GGCGCC HhaI 611 GCGC GCG^C 1675 GCGC Hin1I 1674 GGCGCC G[GA]^CG[CT]C 2224 GGCGTC HinP1I 611 GCGC G^CGC 1675 GCGC MflI 1004 GGATCC [GA]^GATC[CT] 1921 GGATCT MseI 881 TTAA T^TAA 2051 TTAA MvnI 8 CGCG CG^CG 1009 CGCG TaqI 222 TCGA T^CGA 1833 TCGA Tru9I 881 TTAA T^TAA 2051 TTAA TthHB8I 222 TCGA T^CGA 1833 TCGA XcmI 707 CCACGGATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1337 CCAAGGATGAGTTGG XhoII 1004 GGATCC [GA]^GATC[CT] 1921 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbvI BciVI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TseI Tsp45I Tsp509I TspRI XmaCI XmaI