################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MseI MunI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 771 GGTACC G^GTACC AfaI 772 GTAC GT^AC AflIII 461 ACACGT A^C[GA][CT]GT AlwNI 647 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 193 CCTGAGG CC^T[ACGT]AGG Asp718I 771 GGTACC G^GTACC AvaI 498 CTCGGG C^[CT]CG[GA]G BalI 113 TGGCCA TGG^CCA BbeI 805 GGCGCC GGCGC^C BfaI 181 CTAG C^TAG BglI 574 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 520 GGTCTC GGTCTC(1/5) BsgI 703 CTGCAC GTGCAG(16/14) BsoBI 498 CTCGGG C^[CT]CG[GA]G BspMI 512 ACCTGC ACCTGC(4/8) BsrFI 394 ACCGGC [GA]^CCGG[CT] BstAPI 132 GCACGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 372 GGATG GGATG(2/0) Bsu36I 193 CCTGAGG CC^T[ACGT]AGG Cfr10I 394 ACCGGC [GA]^CCGG[CT] DraIII 133 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 472 GACTTCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 708 CCGTGG C^C[GA][CT]GG EarI 388 CTCTTC CTCTTC(1/4) Eco81I 193 CCTGAGG CC^T[ACGT]AGG EcoT14I 243 CCAAGG C^C[AT][AT]GG FokI 372 GGATG GGATG(9/13) HaeII 805 GGCGCC [GA]GCGC^[CT] KasI 805 GGCGCC G^GCGCC KpnI 771 GGTACC GGTAC^C Ksp632I 388 CTCTTC CTCTTC(1/4) MaeI 181 CTAG C^TAG MaeIII 763 GTCAC ^GT[ACGT]AC MluNI 113 TGGCCA TGG^CCA MscI 113 TGGCCA TGG^CCA NarI 805 GGCGCC GG^CGCC NdeI 617 CATATG CA^TATG PstI 745 CTGCAG CTGCA^G RsaI 772 GTAC GT^AC SfoI 805 GGCGCC GGC^GCC StuI 414 AGGCCT AGG^CCT StyI 243 CCAAGG C^C[AT][AT]GG TaqI 299 TCGA T^CGA Tsp45I 763 GTCAC ^GT[GC]AC Tsp509I 339 AATT ^AATT TthHB8I 299 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AspHI 130 GAGCAC G[AT]GC[AT]^C 832 GTGCTC BanI 771 GGTACC G^G[CT][GA]CC 805 GGCGCC BlpI 813 GCTCAGC GC^T[ACGT]AGC 834 GCTCAGC BpmI 273 CTGGAG CTGGAG(16/14) 793 CTGGAG Bpu1102I 813 GCTCAGC GC^T[ACGT]AGC 834 GCTCAGC BseRI 196 GAGGAG GAGGAG(10/8) 440 GAGGAG BsiEI 233 CGGCCG CG[GA][CT]^CG 396 CGGCCG BsiHKAI 130 GAGCAC G[AT]GC[AT]^C 832 GTGCTC BsmBI 480 CGTCTC CGTCTC(1/5) 353 GAGACG BsrBI 101 CCGCTC CCGCTC(-3/-3) 281 CCGCTC BsrDI 203 CATTGC GCAATG(2/0) 592 GCAATG BstYI 108 GGATCT [GA]^GATC[CT] 720 GGATCT CelII 813 GCTCAGC GC^T[ACGT]AGC 834 GCTCAGC CfoI 516 GCGC GCG^C 806 GCGC DraII 501 GGGTCCC [GA]G^G[ACGT]CC[CT] 537 GGGACCT EagI 233 CGGCCG C^GGCCG 396 CGGCCG EclXI 233 CGGCCG C^GGCCG 396 CGGCCG Eco52I 233 CGGCCG C^GGCCG 396 CGGCCG EcoO109I 501 GGGTCCC [GA]G^G[ACGT]CC[CT] 537 GGGACCT HhaI 516 GCGC GCG^C 806 GCGC HinP1I 516 GCGC G^CGC 806 GCGC MaeII 383 ACGT A^CGT 463 ACGT MboII 389 TCTTC GAAGA(8/7) 666 TCTTC MflI 108 GGATCT [GA]^GATC[CT] 720 GGATCT NlaIII 37 CATG CATG^ 531 CATG PpuMI 501 GGGTCCC [GA]G^G[AT]CC[CT] 537 GGGACCT PvuII 175 CAGCTG CAG^CTG 647 CAGCTG SfcI 172 CTACAG C^T[GA][CT]AG 745 CTGCAG XhoII 108 GGATCT [GA]^GATC[CT] 720 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaII BanII BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstUI Cac8I Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI MboI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI TseI TspRI