################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AcyI 466 GGCGCC G[GA]^CG[CT]C AvaI 514 CCCGAG C^[CT]CG[GA]G BanI 466 GGCGCC G^G[CT][GA]CC BbeI 466 GGCGCC GGCGC^C BfaI 148 CTAG C^TAG BpmI 166 CTGGAG CTGGAG(16/14) BsaHI 466 GGCGCC G[GA]^CG[CT]C BsaI 531 GAGACC GGTCTC(1/5) BseRI 13 GAGGAG GAGGAG(10/8) BsmFI 127 GTCCC GGGAC(10/14) BsoBI 514 CCCGAG C^[CT]CG[GA]G Bsp1407I 428 TGTACA T^GTACA BsrDI 53 GCAATG GCAATG(2/0) BsrFI 521 ACCGGC [GA]^CCGG[CT] BsrGI 428 TGTACA T^GTACA BssSI 329 CTCGTG CACGAG(-5/-1) BstXI 413 CCAAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 572 AGATCC [GA]^GATC[CT] CfoI 467 GCGC GCG^C Cfr10I 521 ACCGGC [GA]^CCGG[CT] DdeI 9 CTCAG C^T[ACGT]AG DraII 125 AGGTCCC [GA]G^G[ACGT]CC[CT] EarI 499 CTCTTC CTCTTC(1/4) EcoO109I 125 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 58 GAATTC G^AATTC EcoT14I 392 CCATGG C^C[AT][AT]GG FauI 254 CCCGC CCCGC(4/6) HaeII 466 GGCGCC [GA]GCGC^[CT] HapII 522 CCGG C^CGG HhaI 467 GCGC GCG^C Hin1I 466 GGCGCC G[GA]^CG[CT]C HinP1I 467 GCGC G^CGC HpaII 522 CCGG C^CGG KasI 466 GGCGCC G^GCGCC Ksp632I 499 CTCTTC CTCTTC(1/4) MaeI 148 CTAG C^TAG MflI 572 AGATCC [GA]^GATC[CT] MseI 101 TTAA T^TAA MspA1I 592 CAGCTG C[AC]G^C[GT]G MspI 522 CCGG C^CGG MwoI 410 GCCCCAAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 466 GGCGCC GG^CGCC NcoI 392 CCATGG C^CATGG NspI 494 ACATGC [GA]CATG^[CT] PleI 113 GACTC GAGTC(4/5) PpuMI 125 AGGTCCC [GA]G^G[AT]CC[CT] PstI 595 CTGCAG CTGCA^G PvuII 592 CAGCTG CAG^CTG SapI 498 GCTCTTC GCTCTTC(1/4) SfcI 595 CTGCAG C^T[GA][CT]AG SfoI 466 GGCGCC GGC^GCC SspBI 428 TGTACA T^GTACA StyI 392 CCATGG C^C[AT][AT]GG TaqI 138 TCGA T^CGA TfiI 639 GATTC G^A[AT]TC Tru9I 101 TTAA T^TAA Tsp45I 206 GTGAC ^GT[GC]AC TspRI 480 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 138 TCGA T^CGA XbaI 147 TCTAGA T^CTAGA XcmI 453 CCATCACACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 572 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 255 CCGC CCGC(-3/-1) 608 CCGC AcsI 58 GAATTC [GA]^AATT[CT] 97 AAATTT AlwI 246 GATCC GGATC(4/5) 573 GATCC ApoI 58 GAATTC [GA]^AATT[CT] 97 AAATTT AvaII 126 GGTCC G^G[AT]CC 162 GGACC BanII 43 GGGCTC G[GA]GC[CT]^C 624 GAGCCC BbsI 79 GAAGAC GAAGAC(2/6) 187 GAAGAC BpuAI 79 GAAGAC GAAGAC(2/6) 187 GAAGAC BsmAI 311 GAGAC GTCTC(1/5) 531 GAGAC BspMI 534 ACCTGC ACCTGC(4/8) 123 GCAGGT HinfI 113 GACTC G^A[ACGT]TC 639 GATTC HphI 385 GGTGA GGTGA(8/7) 396 GGTGA MaeIII 206 GTGAC ^GT[ACGT]AC 320 GTTAC MslI 420 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 457 CACACGGATG PflMI 285 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 576 CCATCCGATGG Tsp509I 59 AATT ^AATT 98 AATT Van91I 285 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 576 CCATCCGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AfaI AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DsaI EcoRII Fnu4HI FokI HaeIII ItaI MboI MboII MnlI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI