################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbsI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I KspI MaeI MaeIII MamI MboI MboII MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 313 GACGTC GACGT^C AcsI 160 GAATTC [GA]^AATT[CT] AlwNI 28 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 160 GAATTC [GA]^AATT[CT] AvaII 327 GGTCC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BanI 233 GGCGCC G^G[CT][GA]CC BbeI 233 GGCGCC GGCGC^C BbvCI 211 GCTGAGG CCTCAGC(-5/-2) BciVI 20 GTATCC GTATCC(6/5) BcnI 89 CCCGG CC^[GC]GG BsaWI 341 ACCGGA [AT]^CCGG[AT] BseRI 112 GAGGAG GAGGAG(10/8) BsgI 241 GTGCAG GTGCAG(16/14) BsmFI 12 GTCCC GGGAC(10/14) BsrI 5 CCAGT ACTGG(1/-1) BstXI 291 CCAGCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 234 GCGC GCG^C DraII 319 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 185 CCATGG C^C[GA][CT]GG EcoO109I 319 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 160 GAATTC G^AATTC FauI 48 GCGGG CCCGC(4/6) HaeII 233 GGCGCC [GA]GCGC^[CT] HhaI 234 GCGC GCG^C HinP1I 234 GCGC G^CGC KasI 233 GGCGCC G^GCGCC MaeII 314 ACGT A^CGT MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MspA1I 53 CAGCTG C[AC]G^C[GT]G NarI 233 GGCGCC GG^CGCC NciI 89 CCCGG CC^[GC]GG NcoI 185 CCATGG C^CATGG NspI 248 GCATGC [GA]CATG^[CT] PvuII 53 CAGCTG CAG^CTG SacI 255 GAGCTC GAGCT^C SfaNI 70 GATGC GCATC(5/9) SfoI 233 GGCGCC GGC^GCC SphI 248 GCATGC GCATG^C Tsp509I 161 AATT ^AATT ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcyI 233 GGCGCC G[GA]^CG[CT]C 313 GACGTC AspHI 154 GAGCAC G[AT]GC[AT]^C 255 GAGCTC BanII 189 GGGCTC G[GA]GC[CT]^C 255 GAGCTC BsaHI 233 GGCGCC G[GA]^CG[CT]C 313 GACGTC BsiHKAI 154 GAGCAC G[AT]GC[AT]^C 255 GAGCTC EaeI 2 TGGCCA [CT]^GGCC[GA] 182 CGGCCA EcoT14I 15 CCAAGG C^C[AT][AT]GG 185 CCATGG HapII 90 CCGG C^CGG 342 CCGG Hin1I 233 GGCGCC G[GA]^CG[CT]C 313 GACGTC HpaII 90 CCGG C^CGG 342 CCGG MspI 90 CCGG C^CGG 342 CCGG StyI 15 CCAAGG C^C[AT][AT]GG 185 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstAPI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI