################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MflI MluI MroI MseI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tru9I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 316 GTAC GT^AC AocI 63 CCTGAGG CC^T[ACGT]AGG ApaI 764 GGGCCC GGGCC^C BalI 399 TGGCCA TGG^CCA BanII 764 GGGCCC G[GA]GC[CT]^C BciVI 298 GGATAC GTATCC(6/5) BmyI 764 GGGCCC G[AGT]GC[ACT]^C BsaI 520 GAGACC GGTCTC(1/5) BsiEI 256 CGACCG CG[GA][CT]^CG Bsp1286I 764 GGGCCC G[AGT]GC[ACT]^C BssSI 620 CTCGTG CACGAG(-5/-1) BstAPI 864 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 63 CCTGAGG CC^T[ACGT]AGG EaeI 399 TGGCCA [CT]^GGCC[GA] EarI 415 CTCTTC CTCTTC(1/4) Eco81I 63 CCTGAGG CC^T[ACGT]AGG EcoNI 596 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 22 GACGC GACGC(5/10) Ksp632I 415 CTCTTC CTCTTC(1/4) MaeII 954 ACGT A^CGT MboII 416 TCTTC GAAGA(8/7) MfeI 464 CAATTG C^AATTG MluNI 399 TGGCCA TGG^CCA MscI 399 TGGCCA TGG^CCA MspA1I 647 CCGCTG C[AC]G^C[GT]G MunI 464 CAATTG C^AATTG NaeI 99 GCCGGC GCC^GGC NgoMIV 99 GCCGGC G^CCGGC PpuMI 837 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 522 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 629 CTGCAG CTGCA^G RsaI 316 GTAC GT^AC SpeI 193 ACTAGT A^CTAGT TfiI 174 GAATC G^A[AT]TC Tsp45I 713 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 24 CGCG CG^CG 775 CGCG AcsI 493 AAATTC [GA]^AATT[CT] 721 GAATTT AlwI 637 GATCC GGATC(4/5) 41 GGATC ApoI 493 AAATTC [GA]^AATT[CT] 721 GAATTT AvaII 54 GGACC G^G[AT]CC 838 GGTCC BcnI 33 CCCGG CC^[GC]GG 85 CCCGG BfaI 194 CTAG C^TAG 710 CTAG BglI 253 GCCCGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 744 GCCTCCTTGGC BseRI 705 CTCCTC GAGGAG(10/8) 985 GAGGAG BsmAI 520 GAGAC GTCTC(1/5) 689 GAGAC BsmFI 529 GTCCC GGGAC(10/14) 839 GTCCC BsrFI 99 GCCGGC [GA]^CCGG[CT] 258 ACCGGC BstUI 24 CGCG CG^CG 775 CGCG BstXI 396 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 809 CCAGCAGCCTGG Cfr10I 99 GCCGGC [GA]^CCGG[CT] 258 ACCGGC DsaI 396 CCATGG C^C[GA][CT]GG 402 CCATGG HphI 537 GGTGA GGTGA(8/7) 826 TCACC MaeI 194 CTAG C^TAG 710 CTAG MaeIII 215 GTAAC ^GT[ACGT]AC 713 GTGAC MslI 397 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 616 CACTCTCGTG MvnI 24 CGCG CG^CG 775 CGCG NciI 33 CCCGG CC^[GC]GG 85 CCCGG NcoI 396 CCATGG C^CATGG 402 CCATGG PleI 382 GAGTC GAGTC(4/5) 990 GAGTC SfcI 115 CTACAG C^T[GA][CT]AG 629 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AlwNI BbvI BpmI BsaJI BsiYI BslI BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp509I TthHB8I