################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AccIII AclI AflII AflIII AgeI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstYI BstZ17I ClaI CpoI DpnII DraI DraIII DrdI Eco47III EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MboI MfeI MflI MluI MroI MslI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tth111I XbaI XhoII ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 124 GACGTC GACGT^C Acc65I 971 GGTACC G^GTACC AccI 1102 GTCTAC GT^[AC][GT]AC AccII 528 CGCG CG^CG AhdI 952 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 589 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 798 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 971 GGTACC G^GTACC AspEI 952 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 1559 GGCGCC GGCGC^C BbsI 100 GTCTTC GAAGAC(2/6) BciVI 789 GTATCC GTATCC(6/5) BfaI 1325 CTAG C^TAG BglI 697 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1432 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1432 GCTGAGC GC^T[ACGT]AGC BpuAI 100 GTCTTC GAAGAC(2/6) BsaI 1349 GAGACC GGTCTC(1/5) BsaWI 847 ACCGGA [AT]^CCGG[AT] BsiEI 401 CGGCCG CG[GA][CT]^CG Bsp1407I 1019 TGTACA T^GTACA BsrDI 822 CATTGC GCAATG(2/0) BsrFI 1201 GCCGGC [GA]^CCGG[CT] BsrGI 1019 TGTACA T^GTACA BssSI 768 CACGAG CACGAG(-5/-1) BstUI 528 CGCG CG^CG BstXI 215 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1432 GCTGAGC GC^T[ACGT]AGC Cfr10I 1201 GCCGGC [GA]^CCGG[CT] DsaI 793 CCACGG C^C[GA][CT]GG EagI 401 CGGCCG C^GGCCG Eam1105I 952 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 401 CGGCCG C^GGCCG Eco52I 401 CGGCCG C^GGCCG EcoNI 27 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 517 GATATC GAT^ATC KasI 1559 GGCGCC G^GCGCC KpnI 971 GGTACC GGTAC^C MaeI 1325 CTAG C^TAG MvnI 528 CGCG CG^CG NaeI 1201 GCCGGC GCC^GGC NarI 1559 GGCGCC GG^CGCC NgoMIV 1201 GCCGGC G^CCGGC PaeR7I 827 CTCGAG C^TCGAG PflMI 1366 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 71 GGGACCC [GA]G^G[AT]CC[CT] SfoI 1559 GGCGCC GGC^GCC SspBI 1019 TGTACA T^GTACA StuI 885 AGGCCT AGG^CCT TaqI 828 TCGA T^CGA TfiI 1249 GAATC G^A[AT]TC TthHB8I 828 TCGA T^CGA Van91I 1366 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 821 CCATTGCTCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 827 CTCGAG C^TCGAG XmnI 798 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BanII 646 GAGCTC G[GA]GC[CT]^C 754 GAGCTC BsrBI 235 GAGCGG CCGCTC(-3/-3) 601 GAGCGG BstEII 39 GGTGACC G^GT[ACGT]ACC 273 GGTGACC BstPI 39 GGTGACC G^GT[ACGT]ACC 273 GGTGACC DraII 71 GGGACCC [GA]G^G[ACGT]CC[CT] 391 AGGCCCC EcoO109I 71 GGGACCC [GA]G^G[ACGT]CC[CT] 391 AGGCCCC EcoO65I 39 GGTGACC G^GT[ACGT]ACC 273 GGTGACC EcoRI 853 GAATTC G^AATTC 895 GAATTC FauI 69 GCGGG CCCGC(4/6) 603 GCGGG HaeII 1383 GGCGCT [GA]GCGC^[CT] 1559 GGCGCC HgaI 683 GCGTC GACGC(5/10) 941 GCGTC MseI 179 TTAA T^TAA 836 TTAA PstI 749 CTGCAG CTGCA^G 976 CTGCAG SacI 646 GAGCTC GAGCT^C 754 GAGCTC SfcI 749 CTGCAG C^T[GA][CT]AG 976 CTGCAG SmaI 278 CCCGGG CCC^GGG 380 CCCGGG SmlI 827 CTCGAG C^T[CT][GA]AG 1582 CTCAAG Tru9I 179 TTAA T^TAA 836 TTAA XmaCI 278 CCCGGG C^CCGGG 380 CCCGGG XmaI 278 CCCGGG C^CCGGG 380 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AocI ApoI AspHI AvaI AvaII BalI BanI BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI EaeI EarI Eco81I EcoRII Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI PvuII RsaI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI