(Circular) (Six-base) MAPSORT of: pFN21AE4134.seq Check: 1729 from: 1 to: 6322 pFN21AE4134 6322 bp With 182 enzymes: SgfI * January 19, 2012 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2628 2628 Size: 6322 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2133 4132 278 Size: 53 83 186 936 597 1999 2468 Fragments arranged by size: 2468 1999 936 597 186 83 53 AccI GT'mk_AC Cuts at: 1937 3108 3665 1937 Size: 1171 557 4594 Fragments arranged by size: 4594 1171 557 Acc65I G'GTAC_C Cuts at: 2976 3643 2976 Size: 667 5655 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2558 4385 5769 6037 1632 Size: 635 291 1827 1384 268 1917 Fragments arranged by size: 1917 1827 1384 635 291 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 5221 6198 1203 Size: 4018 977 1327 Fragments arranged by size: 4018 1327 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 2962 5051 2962 Size: 2089 4233 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2313 2323 2769 1184 Size: 1129 10 446 4737 Fragments arranged by size: 4737 1129 446 10 AlwNI CAG_nnn'CTG Cuts at: 2599 4065 5637 2599 Size: 1466 1572 3284 Fragments arranged by size: 3284 1572 1466 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 2490 2858 2900 3631 3857 4009 6118 1493 Size: 997 368 42 731 226 152 2109 1697 Fragments arranged by size: 2109 1697 997 731 368 226 152 42 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2283 2385 2832 3431 3647 1870 Size: 73 340 102 447 599 216 4545 Fragments arranged by size: 4545 599 447 340 216 102 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2449 2482 2449 Size: 33 6289 BamHI G'GATC_C Cuts at: 3652 4019 3652 Size: 367 5955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2976 3497 3564 3643 5098 Size: 325 217 627 1189 521 67 79 1455 Cuts at: 5098 618 Size: 1842 Fragments arranged by size: 1842 1455 1189 627 521 325 217 79 67 BanII G_rGCy'C Cuts at: 1391 2655 2763 3641 1391 Size: 1264 108 878 4072 Fragments arranged by size: 4072 1264 878 108 BbeI G_GCGC'C Cuts at: 3568 3568 Size: 6322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 928 Size: 971 199 5152 Fragments arranged by size: 5152 971 199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4513 4547 1903 Size: 34 2576 34 3678 Fragments arranged by size: 3678 2576 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2805 4214 5424 2805 Size: 1409 1210 3703 Fragments arranged by size: 3703 1409 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2708 4933 136 Size: 107 122 71 631 605 1036 2225 1525 Fragments arranged by size: 2225 1525 1036 631 605 122 107 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3438 3739 3438 Size: 301 6021 Bme1580I G_kGCm'C Cuts at: 1790 3500 4383 5539 1790 Size: 1710 883 1156 2573 Fragments arranged by size: 2573 1710 1156 883 BmgBI CAC'GTC Cuts at: 1318 2013 2104 1318 Size: 695 91 5536 Fragments arranged by size: 5536 695 91 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3121 3241 4053 Size: 416 53 83 399 24 1704 120 812 Cuts at: 4053 5011 442 Size: 958 1753 Fragments arranged by size: 1753 1704 958 812 416 399 120 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 2663 2750 4982 1239 Size: 1292 132 87 2232 2579 Fragments arranged by size: 2579 2232 1292 132 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3572 4447 5312 5610 3572 Size: 875 865 298 4284 Fragments arranged by size: 4284 875 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3348 4985 882 Size: 617 1849 1637 2219 Fragments arranged by size: 2219 1849 1637 617 BsaAI yAC'GTr Cuts at: 493 1204 4113 493 Size: 711 2909 2702 Fragments arranged by size: 2909 2702 711 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2130 2688 3565 4129 Size: 53 83 186 936 597 558 877 564 Cuts at: 4129 4511 5146 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 877 635 597 564 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2852 4755 5153 5427 5574 5985 2852 Size: 1903 398 274 147 411 3189 Fragments arranged by size: 3189 1903 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3125 3155 1178 Size: 30 165 30 1722 30 4345 Fragments arranged by size: 4345 1722 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2864 3182 3200 3203 3206 3215 3296 Size: 868 810 318 18 3 3 9 81 Cuts at: 3296 3299 3308 3391 3394 1186 Size: 3 9 83 3 4114 Fragments arranged by size: 4114 868 810 318 83 81 18 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2430 3048 3076 3448 5525 6021 2430 Size: 618 28 372 2077 496 2731 Fragments arranged by size: 2731 2077 618 496 372 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2104 2315 3026 1163 Size: 573 368 211 711 4459 Fragments arranged by size: 4459 711 573 368 211 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2409 3772 4533 5561 1540 Size: 275 186 408 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 408 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 2655 2763 4383 4468 5539 2394 Size: 261 108 1620 85 1071 3177 Fragments arranged by size: 3177 1620 1071 261 108 85 BsmI GAATG_Cn' Cuts at: 3833 3926 3833 Size: 93 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2655 2763 3500 3641 4383 4468 Size: 399 604 261 108 737 141 742 85 Cuts at: 4468 5539 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 737 604 399 261 141 108 85 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 4211 5941 4211 Size: 1730 4592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 2079 2628 2988 3048 3677 844 Size: 1213 22 549 360 60 629 3489 Fragments arranged by size: 3489 1213 629 549 360 60 22 BsrBI CCG'CTC Cuts at: 1394 1823 2242 2608 4209 1394 Size: 429 419 366 1601 3507 Fragments arranged by size: 3507 1601 429 419 366 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 4817 4991 65 Size: 1183 1578 1991 174 1396 Fragments arranged by size: 1991 1578 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3206 4966 5153 1700 Size: 110 1396 1760 187 2869 Fragments arranged by size: 2869 1760 1396 187 110 BsrGI T'GTAC_A Cuts at: 96 3024 96 Size: 2928 3394 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 2773 4382 5394 2773 Size: 1609 1012 3701 Fragments arranged by size: 3701 1609 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6322 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 2044 2278 1184 Size: 860 234 5228 Fragments arranged by size: 5228 860 234 BstXI CCAn_nnnn'nTGG Cuts at: 2227 2227 Size: 6322 BstYI r'GATC_y Cuts at: 1972 3652 4019 4404 4421 5873 5959 5971 6317 Size: 1680 367 385 17 1452 86 12 346 Cuts at: 6317 1972 Size: 1977 Fragments arranged by size: 1977 1680 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2471 2600 2894 2471 Size: 129 294 5899 Fragments arranged by size: 5899 294 129 BtgI C'CryG_G Cuts at: 513 1065 2004 2798 513 Size: 552 939 794 4037 Fragments arranged by size: 4037 939 794 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3279 3460 487 Size: 683 56 2053 181 3349 Fragments arranged by size: 3349 2053 683 181 56 BtsI GCAGTG_nn' Cuts at: 3452 3546 3838 4632 4652 3452 Size: 94 292 794 20 5122 Fragments arranged by size: 5122 794 292 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 3626 3626 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2098 2217 Size: 54 1425 50 275 18 177 91 119 Cuts at: 2217 2406 2529 2967 3368 3593 3769 4658 6287 Size: 189 123 438 401 225 176 889 1629 Cuts at: 6287 8 Size: 43 Fragments arranged by size: 1629 1425 889 438 401 275 225 189 177 176 123 119 91 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2406 3769 1537 Size: 275 594 1363 4090 Fragments arranged by size: 4090 1363 594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 3150 3168 3177 3192 3264 3273 3285 Size: 678 1119 18 9 15 72 9 12 Cuts at: 3285 3338 4252 1353 Size: 53 914 3423 Fragments arranged by size: 3423 1119 914 678 72 53 18 15 12 9 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2123 4909 5283 5429 387 Size: 1064 138 534 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 534 374 146 138 EcoICRI GAG'CTC Cuts at: 2653 2761 2653 Size: 108 6214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2531 2558 2663 2750 4385 4982 Size: 393 635 264 27 105 87 1635 597 Cuts at: 4982 5769 6037 1239 Size: 787 268 1524 Fragments arranged by size: 1635 1524 787 635 597 393 268 264 105 87 27 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2036 Size: 6322 EcoO109I rG'GnC_Cy Cuts at: 2077 2397 2077 Size: 320 6002 EcoRI G'AATT_C Cuts at: 2858 2900 3631 4009 2858 Size: 42 731 378 5171 Fragments arranged by size: 5171 731 378 42 EcoRV GAT'ATC Cuts at: 2524 2524 Size: 6322 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 3392 3568 5469 1678 Size: 1714 176 1901 2531 Fragments arranged by size: 2531 1901 1714 176 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2948 2980 3125 3157 3239 Size: 32 135 32 1376 32 145 32 82 Cuts at: 3239 3271 3459 3491 4963 4995 5037 5069 1373 Size: 32 188 32 1472 32 42 32 2626 Fragments arranged by size: 2626 1472 1376 188 145 135 82 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6322 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KasI G'GCGC_C Cuts at: 3564 3564 Size: 6322 KpnI G_GTAC'C Cuts at: 2980 3647 2980 Size: 667 5655 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2224 5436 5620 1305 Size: 919 3212 184 2007 Fragments arranged by size: 3212 2007 919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2100 2219 3595 10 Size: 54 1425 343 177 91 119 1376 2737 Fragments arranged by size: 2737 1425 1376 343 177 119 91 54 MslI CAynn'nnrTG Cuts at: 518 1166 4280 4639 4798 518 Size: 648 3114 359 159 2042 Fragments arranged by size: 3114 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2632 2753 2986 3738 4415 5563 5808 Size: 512 514 121 233 752 677 1148 245 Cuts at: 5808 1606 Size: 2120 Fragments arranged by size: 2120 1148 752 677 514 512 245 233 121 NaeI GCC'GGC Cuts at: 3208 3208 Size: 6322 NarI GG'CG_CC Cuts at: 3565 3565 Size: 6322 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4831 Fragments arranged by size: 4831 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NgoMIV G'CCGG_C Cuts at: 3206 3206 Size: 6322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 3680 5225 1132 Size: 97 2451 1545 2229 Fragments arranged by size: 2451 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6322 PciI A'CATG_T Cuts at: 5221 5221 Size: 6322 PflMI CCAn_nnn'nTGG Cuts at: 1108 3377 1108 Size: 2269 4053 PfoI T'CCnGG_A Cuts at: 2547 2547 Size: 6322 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2077 2077 Size: 6322 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PspXI vC'TCGA_Gb Cuts at: 2832 2832 Size: 6322 PstI C_TGCA'G Cuts at: 830 2758 2985 3674 830 Size: 1928 227 689 3478 Fragments arranged by size: 3478 1928 689 227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 PvuII CAG'CTG Cuts at: 2118 2632 2753 2986 2118 Size: 514 121 233 5454 Fragments arranged by size: 5454 514 233 121 SacI G_AGCT'C Cuts at: 2655 2763 2655 Size: 108 6214 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SanDI GG'GwC_CC Cuts at: 2077 2077 Size: 6322 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SfcI C'TryA_G Cuts at: 826 1045 2754 2981 3670 4805 5486 5677 826 Size: 219 1709 227 689 1135 681 191 1471 Fragments arranged by size: 1709 1471 1135 689 681 227 219 191 SfoI GGC'GCC Cuts at: 3566 3566 Size: 6322 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 2285 2387 3649 1872 Size: 413 102 1262 4545 Fragments arranged by size: 4545 1262 413 102 SmlI C'TyrA_G Cuts at: 820 1017 1943 2832 3587 4426 5327 5589 820 Size: 197 926 889 755 839 901 262 1553 Fragments arranged by size: 1553 926 901 889 839 755 262 197 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 3680 1132 Size: 2548 3774 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 2892 1517 Size: 1375 4947 StyI C'CwwG_G Cuts at: 513 1065 2004 3761 513 Size: 552 939 1757 3074 Fragments arranged by size: 3074 1757 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4349 4366 4519 4704 5149 325 Size: 1255 2769 17 153 185 445 1498 Fragments arranged by size: 2769 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2562 3024 4568 Size: 274 80 33 51 1443 585 462 1544 Cuts at: 4568 6280 96 Size: 1712 138 Fragments arranged by size: 1712 1544 1443 585 462 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2987 3019 3368 3400 6282 6314 2987 Size: 32 349 32 2882 32 2995 Fragments arranged by size: 2995 2882 349 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6322 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2833 2833 Size: 6322 XhoI C'TCGA_G Cuts at: 1943 2832 1943 Size: 889 5433 XmaI C'CCGG_G Cuts at: 1870 2283 2385 3647 1870 Size: 413 102 1262 4545 Fragments arranged by size: 4545 1262 413 102 XmnI GAAnn'nnTTC Cuts at: 2807 4451 2807 Size: 1644 4678 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2131 4130 276 Size: 53 83 186 936 597 1999 2468 Fragments arranged by size: 2468 1999 936 597 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI