(Linear) (Six-base) MAPSORT of: KIEE4134.seq Check: 2717 from: 1 to: 1617 KIEE4134.orf With 182 enzymes: SgfI * January 19, 2012 13:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 623 1617 Size: 623 994 AatII G_ACGT'C Cuts at: 0 128 1617 Size: 128 1489 AccI GT'mk_AC Cuts at: 0 1103 1617 Size: 1103 514 Acc65I G'GTAC_C Cuts at: 0 971 1617 Size: 971 646 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 553 1617 Size: 262 291 1064 Fragments arranged by size: 1064 291 262 AhdI GACnn_n'nnGTC Cuts at: 0 957 1617 Size: 957 660 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 308 318 764 1617 Size: 308 10 446 853 Fragments arranged by size: 853 446 308 10 AlwNI CAG_nnn'CTG Cuts at: 0 594 1617 Size: 594 1023 ApoI r'AATT_y Cuts at: 0 485 853 895 1617 Size: 485 368 42 722 Fragments arranged by size: 722 485 368 42 AvaI C'yCGr_G Cuts at: 0 278 380 827 1426 1617 Size: 278 102 447 599 191 Fragments arranged by size: 599 447 278 191 102 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 444 477 1617 Size: 444 33 1140 Fragments arranged by size: 1140 444 33 BanI G'GyrC_C Cuts at: 0 971 1492 1559 1617 Size: 971 521 67 58 Fragments arranged by size: 971 521 67 58 BanII G_rGCy'C Cuts at: 0 650 758 1617 Size: 650 108 859 Fragments arranged by size: 859 650 108 BbeI G_GCGC'C Cuts at: 0 1563 1617 Size: 1563 54 BbsI GAAGACnn'nnnn_ Cuts at: 0 93 1617 Size: 93 1524 BciVI GTATCCnnnnn_n' Cuts at: 0 800 1617 Size: 800 817 BglI GCCn_nnn'nGGC Cuts at: 0 703 1617 Size: 703 914 BlpI GC'TnA_GC Cuts at: 0 1433 1617 Size: 1433 184 Bme1580I G_kGCm'C Cuts at: 0 1495 1617 Size: 1495 122 BmgBI CAC'GTC Cuts at: 0 8 99 1617 Size: 8 91 1518 Fragments arranged by size: 1518 91 8 BmrI ACTGGGnnnn_n' Cuts at: 0 1116 1236 1617 Size: 1116 120 381 Fragments arranged by size: 1116 381 120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 526 658 745 1617 Size: 526 132 87 872 Fragments arranged by size: 872 526 132 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1567 1617 Size: 1567 50 BsaI GGTCTCn'nnnn_ Cuts at: 0 1343 1617 Size: 1343 274 BsaHI Gr'CG_yC Cuts at: 0 125 683 1560 1617 Size: 125 558 877 57 Fragments arranged by size: 877 558 125 57 BsaWI w'CCGG_w Cuts at: 0 847 1617 Size: 847 770 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1120 1150 1617 Size: 1120 30 467 Fragments arranged by size: 1120 467 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 859 1177 1195 1198 1201 1210 1291 Size: 49 810 318 18 3 3 9 81 Cuts at: 1291 1294 1303 1386 1389 1617 Size: 3 9 83 3 228 Fragments arranged by size: 810 318 228 83 81 49 18 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 425 1043 1071 1443 1617 Size: 425 618 28 372 174 Fragments arranged by size: 618 425 372 174 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 99 310 1021 1617 Size: 99 211 711 596 Fragments arranged by size: 711 596 211 99 BsiEI CG_ry'CG Cuts at: 0 404 1617 Size: 404 1213 BsiHKAI G_wGCw'C Cuts at: 0 389 650 758 1617 Size: 389 261 108 859 Fragments arranged by size: 859 389 261 108 Bsp1286I G_dGCh'C Cuts at: 0 389 650 758 1495 1617 Size: 389 261 108 737 122 Fragments arranged by size: 737 389 261 122 108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 52 74 623 983 1043 1617 Size: 52 22 549 360 60 574 Fragments arranged by size: 574 549 360 60 52 22 BsrBI CCG'CTC Cuts at: 0 237 603 1617 Size: 237 366 1014 Fragments arranged by size: 1014 366 237 BsrDI GCAATG_nn' Cuts at: 0 821 1617 Size: 821 796 BsrFI r'CCGG_y Cuts at: 0 1201 1617 Size: 1201 416 BsrGI T'GTAC_A Cuts at: 0 1019 1617 Size: 1019 598 BssSI C'ACGA_G Cuts at: 0 768 1617 Size: 768 849 BstEII G'GTnAC_C Cuts at: 0 39 273 1617 Size: 39 234 1344 Fragments arranged by size: 1344 234 39 BstXI CCAn_nnnn'nTGG Cuts at: 0 222 1617 Size: 222 1395 Bsu36I CC'TnA_GG Cuts at: 0 466 595 889 1617 Size: 466 129 294 728 Fragments arranged by size: 728 466 294 129 BtgI C'CryG_G Cuts at: 0 793 1617 Size: 793 824 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1274 1455 1617 Size: 1274 181 162 Fragments arranged by size: 1274 181 162 BtsI GCAGTG_nn' Cuts at: 0 1447 1541 1617 Size: 1447 94 76 Fragments arranged by size: 1447 94 76 EaeI y'GGCC_r Cuts at: 0 2 93 212 401 524 962 1363 1588 Size: 2 91 119 189 123 438 401 225 Cuts at: 1588 1617 Size: 29 Fragments arranged by size: 438 401 225 189 123 119 91 29 2 EagI C'GGCC_G Cuts at: 0 401 1617 Size: 401 1216 EarI CTCTTCn'nnn_ Cuts at: 0 26 1145 1163 1172 1187 1259 1268 1280 Size: 26 1119 18 9 15 72 9 12 Cuts at: 1280 1333 1617 Size: 53 284 Fragments arranged by size: 1119 284 72 53 26 18 15 12 9 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 118 1617 Size: 118 1499 EcoICRI GAG'CTC Cuts at: 0 648 756 1617 Size: 648 108 861 Fragments arranged by size: 861 648 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 526 553 658 745 1617 Size: 262 264 27 105 87 872 Fragments arranged by size: 872 264 262 105 87 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 31 1617 Size: 31 1586 EcoO109I rG'GnC_Cy Cuts at: 0 72 392 1617 Size: 72 320 1225 Fragments arranged by size: 1225 320 72 EcoRI G'AATT_C Cuts at: 0 853 895 1617 Size: 853 42 722 Fragments arranged by size: 853 722 42 EcoRV GAT'ATC Cuts at: 0 519 1617 Size: 519 1098 HaeII r_GCGC'y Cuts at: 0 1387 1563 1617 Size: 1387 176 54 Fragments arranged by size: 1387 176 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 943 975 1120 1152 1234 1266 1454 1486 Size: 943 32 145 32 82 32 188 32 Cuts at: 1486 1617 Size: 131 Fragments arranged by size: 943 188 145 131 82 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1559 1617 Size: 1559 58 KpnI G_GTAC'C Cuts at: 0 975 1617 Size: 975 642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 219 1617 Size: 219 1398 MscI TGG'CCA Cuts at: 0 4 95 214 1590 1617 Size: 4 91 119 1376 27 Fragments arranged by size: 1376 119 91 27 4 MspA1I CmG'CkG Cuts at: 0 113 627 748 981 1617 Size: 113 514 121 233 636 Fragments arranged by size: 636 514 233 121 113 NaeI GCC'GGC Cuts at: 0 1203 1617 Size: 1203 414 NarI GG'CG_CC Cuts at: 0 1560 1617 Size: 1560 57 NgoMIV G'CCGG_C Cuts at: 0 1201 1617 Size: 1201 416 PflMI CCAn_nnn'nTGG Cuts at: 0 1372 1617 Size: 1372 245 PfoI T'CCnGG_A Cuts at: 0 542 1617 Size: 542 1075 PpuMI rG'GwC_Cy Cuts at: 0 72 1617 Size: 72 1545 PspXI vC'TCGA_Gb Cuts at: 0 827 1617 Size: 827 790 PstI C_TGCA'G Cuts at: 0 753 980 1617 Size: 753 227 637 Fragments arranged by size: 753 637 227 PvuII CAG'CTG Cuts at: 0 113 627 748 981 1617 Size: 113 514 121 233 636 Fragments arranged by size: 636 514 233 121 113 SacI G_AGCT'C Cuts at: 0 650 758 1617 Size: 650 108 859 Fragments arranged by size: 859 650 108 SanDI GG'GwC_CC Cuts at: 0 72 1617 Size: 72 1545 SfcI C'TryA_G Cuts at: 0 749 976 1617 Size: 749 227 641 Fragments arranged by size: 749 641 227 SfoI GGC'GCC Cuts at: 0 1561 1617 Size: 1561 56 SmaI CCC'GGG Cuts at: 0 280 382 1617 Size: 280 102 1235 Fragments arranged by size: 1235 280 102 SmlI C'TyrA_G Cuts at: 0 827 1582 1617 Size: 827 755 35 Fragments arranged by size: 827 755 35 StuI AGG'CCT Cuts at: 0 887 1617 Size: 887 730 TatI w'GTAC_w Cuts at: 0 557 1019 1617 Size: 557 462 598 Fragments arranged by size: 598 557 462 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 982 1014 1363 1395 1617 Size: 982 32 349 32 222 Fragments arranged by size: 982 349 222 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 828 1617 Size: 828 789 XhoI C'TCGA_G Cuts at: 0 827 1617 Size: 827 790 XmaI C'CCGG_G Cuts at: 0 278 380 1617 Size: 278 102 1237 Fragments arranged by size: 1237 278 102 XmnI GAAnn'nnTTC Cuts at: 0 802 1617 Size: 802 815 ZraI GAC'GTC Cuts at: 0 126 1617 Size: 126 1491 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AhdI AlfI AlwNI ApoI AvaI BaeI BanI BanII BbeI BbsI BciVI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I KasI KpnI MmeI MscI MspA1I NaeI NarI NgoMIV PflMI PfoI PpuMI PspXI PstI PvuII SacI SanDI SfcI SfoI SmaI SmlI StuI TatI TstI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AclI AfeI AflII AflIII AgeI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbvCI BcgI BclI BfrBI BglII BmtI BplI Bpu10I BsaAI BsaBI BsiWI BsmI BsmBI BspEI BspHI BssHII BstAPI BstBI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII Tth111I XbaI