################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 249 AAATTT [GA]^AATT[CT] AlwI 207 GGATC GGATC(4/5) ApoI 249 AAATTT [GA]^AATT[CT] Asp700I 331 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 610 GAGCTC G[AT]GC[AT]^C AvaII 804 GGACC G^G[AT]CC BanII 610 GAGCTC G[GA]GC[CT]^C BciVI 680 GGATAC GTATCC(6/5) BglI 316 GCCTTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 610 GAGCTC G[AGT]GC[ACT]^C BsgI 545 CTGCAC GTGCAG(16/14) BsiHKAI 610 GAGCTC G[AT]GC[AT]^C BsmBI 765 CGTCTC CGTCTC(1/5) Bsp1286I 610 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 226 TGTACA T^GTACA BspMI 776 ACCTGC ACCTGC(4/8) BsrDI 840 CATTGC GCAATG(2/0) BsrGI 226 TGTACA T^GTACA BstYI 207 GGATCT [GA]^GATC[CT] Cfr13I 804 GGACC G^G[ACGT]CC DraI 901 TTTAAA TTT^AAA DraIII 500 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 344 TGGCCG [CT]^GGCC[GA] EcoNI 356 CCTTTGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 698 GTCGAC GT[CT]^[GA]AC HindII 698 GTCGAC GT[CT]^[GA]AC MflI 207 GGATCT [GA]^GATC[CT] NdeI 650 CATATG CA^TATG NlaIV 724 GGTTCC GG[ACGT]^[ACGT]CC SacI 610 GAGCTC GAGCT^C SalI 698 GTCGAC G^TCGAC SapI 612 GCTCTTC GCTCTTC(1/4) Sau96I 804 GGACC G^G[ACGT]CC SmlI 553 CTCAAG C^T[CT][GA]AG SspBI 226 TGTACA T^GTACA XhoII 207 GGATCT [GA]^GATC[CT] XmnI 331 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 477 GTCTAC GT^[AC][GT]AC 698 GTCGAC AflIII 223 ACATGT A^C[GA][CT]GT 718 ACATGT BbsI 88 GTCTTC GAAGAC(2/6) 922 GTCTTC BfaI 139 CTAG C^TAG 244 CTAG BpuAI 88 GTCTTC GAAGAC(2/6) 922 GTCTTC BsiYI 356 CCTTTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 423 CCTCCAGTTGG BslI 356 CCTTTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 423 CCTCCAGTTGG BspLU11I 223 ACATGT A^CATGT 718 ACATGT BsrI 451 ACTGG ACTGG(1/-1) 426 CCAGT BstF5I 440 CATCC GGATG(2/0) 639 CATCC EarI 613 CTCTTC CTCTTC(1/4) 826 CTCTTC FokI 440 CATCC GGATG(9/13) 639 CATCC Ksp632I 613 CTCTTC CTCTTC(1/4) 826 CTCTTC MaeI 139 CTAG C^TAG 244 CTAG MseI 902 TTAA T^TAA 928 TTAA MspA1I 434 CAGCTG C[AC]G^C[GT]G 493 CAGCTG MwoI 316 GCCTTGGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 580 GCAAATGAGGC NspI 223 ACATGT [GA]CATG^[CT] 718 ACATGT PvuII 434 CAGCTG CAG^CTG 493 CAGCTG TaqI 57 TCGA T^CGA 699 TCGA TfiI 836 GAATC G^A[AT]TC 855 GAATC Tru9I 902 TTAA T^TAA 928 TTAA TthHB8I 57 TCGA T^CGA 699 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AfaI AluI BbvI BpmI BsaJI BsmAI BsmFI BssKI BstNI DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MvaI NdeII NlaIII PleI RsaI Sau3AI ScrFI SfaNI StyI TseI Tsp45I Tsp509I TspRI