(Circular) (Six-base) MAPSORT of: pFN21AE5443.seq Check: 1395 from: 1 to: 5644 pFN21AE5443 5644 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2483 2704 2987 1937 Size: 546 221 283 4594 Fragments arranged by size: 4594 546 283 221 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2718 3707 5091 5359 1632 Size: 1086 989 1384 268 1917 Fragments arranged by size: 1917 1384 1086 989 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2228 2723 4543 5520 1203 Size: 1025 495 1820 977 1327 Fragments arranged by size: 1820 1327 1025 977 495 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2713 2745 2713 Size: 32 5612 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2254 2953 3179 3331 5440 1493 Size: 761 699 226 152 2109 1697 Fragments arranged by size: 2109 1697 761 699 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 4420 618 Size: 325 217 627 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 325 217 BanII G_rGCy'C Cuts at: 1391 2619 2963 1391 Size: 1228 344 4072 Fragments arranged by size: 4072 1228 344 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 2920 928 Size: 971 187 834 3652 Fragments arranged by size: 3652 971 834 187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2679 3536 4746 2679 Size: 857 1210 3577 Fragments arranged by size: 3577 1210 857 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2327 4255 136 Size: 107 122 71 631 605 655 1928 1525 Fragments arranged by size: 1928 1525 655 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 3705 4861 1790 Size: 1915 1156 2573 Fragments arranged by size: 2573 1915 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2414 2480 4304 1239 Size: 854 321 66 1824 2579 Fragments arranged by size: 2579 1824 854 321 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 3769 4634 4932 2543 Size: 1226 865 298 3255 Fragments arranged by size: 3255 1226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2412 2442 1178 Size: 30 165 30 1009 30 4380 Fragments arranged by size: 4380 1009 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5644 BseYI C'CCAG_C Cuts at: 2047 2301 2798 4847 5343 2047 Size: 254 497 2049 496 2348 Fragments arranged by size: 2348 2049 497 496 254 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2535 1163 Size: 573 799 4272 Fragments arranged by size: 4272 799 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2619 3705 3790 4861 2619 Size: 1086 85 1071 3402 Fragments arranged by size: 3402 1086 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 BsmBI CGTCTCn'nnnn_ Cuts at: 2776 2776 Size: 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2619 2963 3705 3790 4861 1391 Size: 399 829 344 742 85 1071 2174 Fragments arranged by size: 2174 1071 829 742 399 344 85 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2790 2999 844 Size: 1946 209 3489 Fragments arranged by size: 3489 1946 209 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2844 4139 4313 65 Size: 1183 1596 1295 174 1396 Fragments arranged by size: 1596 1396 1295 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 2231 96 Size: 2135 3509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2212 2974 3341 3726 3743 5195 5281 5293 Size: 240 762 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 762 385 367 346 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5092 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 2552 3160 3954 3974 2552 Size: 608 794 20 4222 Fragments arranged by size: 4222 794 608 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2908 2948 2908 Size: 40 5604 DraIII CAC_nnn'GTG Cuts at: 2510 2510 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 3091 3980 Size: 54 1425 50 275 18 519 742 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 742 519 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2624 2837 3574 1353 Size: 1271 213 737 3423 Fragments arranged by size: 3423 1271 737 213 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2617 2617 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2414 2480 2718 3707 4304 5091 Size: 393 461 321 66 238 989 597 787 Cuts at: 5091 5359 1239 Size: 268 1524 Fragments arranged by size: 1524 989 787 597 461 393 321 268 238 66 EcoNI CCTnn'n_nnAGG Cuts at: 2365 2365 Size: 5644 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2920 2920 Size: 5644 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 4791 1678 Size: 3113 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2412 2444 2812 2844 4285 Size: 32 135 32 840 32 368 32 1441 Cuts at: 4285 4317 4359 4391 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1441 840 368 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2705 2988 3234 669 Size: 988 281 767 283 246 3079 Fragments arranged by size: 3079 988 767 283 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3822 Fragments arranged by size: 3822 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3602 3961 4120 518 Size: 648 2436 359 159 2042 Fragments arranged by size: 2436 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2441 2500 3060 3737 4885 5130 1606 Size: 835 59 560 677 1148 245 2120 Fragments arranged by size: 2120 1148 835 677 560 245 59 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5092 NdeI CA'TA_TG Cuts at: 387 2656 387 Size: 2269 3375 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2232 2727 3002 4547 1132 Size: 97 1003 495 275 1545 2229 Fragments arranged by size: 2229 1545 1003 495 275 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 2228 2723 4543 2228 Size: 495 1820 3329 Fragments arranged by size: 3329 1820 495 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4742 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2996 830 Size: 2166 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2441 2500 2441 Size: 59 5585 SacI G_AGCT'C Cuts at: 2619 2619 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2703 2986 1936 Size: 767 283 4594 Fragments arranged by size: 4594 767 283 SapI GCTCTTCn'nnn_ Cuts at: 2624 2624 Size: 5644 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2992 4127 4808 4999 826 Size: 219 1947 1135 681 191 1471 Fragments arranged by size: 1947 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 3748 4649 4911 820 Size: 197 926 615 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 615 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2066 2322 2805 2812 3083 513 Size: 552 1001 256 483 7 271 3074 Fragments arranged by size: 3074 1001 552 483 271 256 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2866 3671 3688 3841 4026 4471 325 Size: 1255 1286 805 17 153 185 445 1498 Fragments arranged by size: 1498 1286 1255 805 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2231 2762 3890 Size: 274 80 33 51 1443 254 531 1128 Cuts at: 3890 5602 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1128 531 274 254 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 2340 3773 2340 Size: 1433 4211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI