################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AfaI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BclI BfaI BfrI BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MroI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SapI ScaI SexAI SfaNI SfoI SfuI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccI 171 GTCGAC GT^[AC][GT]AC AgeI 34 ACCGGT A^CCGGT AlwI 186 GATCC GGATC(4/5) BalI 62 TGGCCA TGG^CCA BanI 133 GGCACC G^G[CT][GA]CC BanII 307 GGGCTC G[GA]GC[CT]^C BcgI 173 CGACTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 318 GGATAC GTATCC(6/5) BcnI 130 CCCGG CC^[GC]GG BglII 243 AGATCT A^GATCT BlpI 343 GCTAAGC GC^T[ACGT]AGC BmyI 307 GGGCTC G[AGT]GC[ACT]^C Bpu1102I 343 GCTAAGC GC^T[ACGT]AGC BsaWI 34 ACCGGT [AT]^CCGG[AT] BsgI 20 CTGCAC GTGCAG(16/14) BsmFI 128 GTCCC GGGAC(10/14) BsmI 389 GCATTC GAATGC(1/-1) Bsp1286I 307 GGGCTC G[AGT]GC[ACT]^C BssHII 86 GCGCGC G^CGCGC BstF5I 336 CATCC GGATG(2/0) BstNI 210 CCTGG CC^[AT]GG BstYI 243 AGATCT [GA]^GATC[CT] CelII 343 GCTAAGC GC^T[ACGT]AGC EcoNI 276 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 210 CCTGG ^CC[AT]GG EcoT14I 365 CCAAGG C^C[AT][AT]GG FauI 5 CCCGC CCCGC(4/6) FokI 336 CATCC GGATG(9/13) HaeII 45 AGCGCC [GA]GCGC^[CT] HincII 171 GTCGAC GT[CT]^[GA]AC HindII 171 GTCGAC GT[CT]^[GA]AC HinfI 153 GATTC G^A[ACGT]TC HphI 267 GGTGA GGTGA(8/7) KspI 263 CCGCGG CCGC^GG MaeIII 352 GTGAC ^GT[ACGT]AC MboII 108 GAAGA GAAGA(8/7) MflI 243 AGATCT [GA]^GATC[CT] MluNI 62 TGGCCA TGG^CCA MscI 62 TGGCCA TGG^CCA MvaI 210 CCTGG CC^[AT]GG NaeI 90 GCCGGC GCC^GGC NciI 130 CCCGG CC^[GC]GG NgoMIV 90 GCCGGC G^CCGGC NlaIV 133 GGCACC GG[ACGT]^[ACGT]CC PflMI 204 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 34 ACCGGT A^CCGGT PstI 277 CTGCAG CTGCA^G PvuII 58 CAGCTG CAG^CTG SacII 263 CCGCGG CCGC^GG SalI 171 GTCGAC G^TCGAC SbfI 276 CCTGCAGG CCTGCA^GG SfcI 277 CTGCAG C^T[GA][CT]AG SfiI 285 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 276 CCTGCAGG CCTGCA^GG StyI 365 CCAAGG C^C[AT][AT]GG TaqI 172 TCGA T^CGA TfiI 153 GATTC G^A[AT]TC Tsp45I 352 GTGAC ^GT[GC]AC TthHB8I 172 TCGA T^CGA Van91I 204 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 243 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BbvI 57 GCAGC GCAGC(8/12) 180 GCTGC BglI 64 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 286 GCCTGCGAGGC BssKI 130 CCCGG ^CC[ACGT]GG 210 CCTGG Cfr13I 3 GGCCC G^G[ACGT]CC 273 GGCCC DpnII 186 GATC ^GATC 244 GATC DsaI 137 CCGTGG C^C[GA][CT]GG 263 CCGCGG EaeI 62 TGGCCA [CT]^GGCC[GA] 92 CGGCCA MboI 186 GATC ^GATC 244 GATC MspA1I 58 CAGCTG C[AC]G^C[GT]G 263 CCGCGG NdeII 186 GATC ^GATC 244 GATC NlaIII 340 CATG CATG^ 360 CATG Sau3AI 186 GATC ^GATC 244 GATC Sau96I 3 GGCCC G^G[ACGT]CC 273 GGCCC ScrFI 130 CCCGG CC^[ACGT]GG 210 CCTGG SgrAI 33 CACCGGTG C[GA]^CCGG[CT]G 96 CACCGGCG StuI 284 AGGCCT AGG^CCT 293 AGGCCT TseI 57 GCAGC G^C[AT]GC 180 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AccII AciI AluI BsaJI BsiYI BslI BsrFI BstUI Cac8I CfoI Cfr10I DdeI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvnI MwoI