################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstPI BstUI Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HindIII HinfI KasI Ksp632I KspI MaeI MaeII MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### Acc65I 343 GGTACC G^GTACC AccI 363 GTATAC GT^[AC][GT]AC AciI 290 CCGC CCGC(-3/-1) AcsI 189 GAATTC [GA]^AATT[CT] AlwI 282 GATCC GGATC(4/5) AlwNI 20 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 257 AGCGCT AGC^GCT ApoI 189 GAATTC [GA]^AATT[CT] Asp700I 189 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 343 GGTACC G^GTACC BalI 320 TGGCCA TGG^CCA BanI 343 GGTACC G^G[CT][GA]CC BbvI 303 GCTGC GCAGC(8/12) BcnI 310 CCCGG CC^[GC]GG BpmI 273 CTCCAG CTGGAG(16/14) Bsp1407I 125 TGTACA T^GTACA BspHI 185 TCATGA T^CATGA BsrGI 125 TGTACA T^GTACA BsrI 157 ACTGG ACTGG(1/-1) Bst1107I 363 GTATAC GTA^TAC BstBI 211 TTCGAA TT^CGAA BstNI 275 CCAGG CC^[AT]GG BstXI 170 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 281 AGATCC [GA]^GATC[CT] BstZ17I 363 GTATAC GTA^TAC Cfr13I 243 GGCCC G^G[ACGT]CC DdeI 14 CTAAG C^T[ACGT]AG DpnII 282 GATC ^GATC DraIII 328 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 176 CCATGG C^C[GA][CT]GG EaeI 320 TGGCCA [CT]^GGCC[GA] Eco47III 257 AGCGCT AGC^GCT EcoRI 189 GAATTC G^AATTC EcoRII 275 CCAGG ^CC[AT]GG Fnu4HI 303 GCTGC GC^[ACGT]GC HaeII 257 AGCGCT [GA]GCGC^[CT] HapII 311 CCGG C^CGG HgaI 297 GCGTC GACGC(5/10) HincII 199 GTTAAC GT[CT]^[GA]AC HindII 199 GTTAAC GT[CT]^[GA]AC HpaI 199 GTTAAC GTT^AAC HpaII 311 CCGG C^CGG ItaI 303 GCTGC GC^[ACGT]GC KpnI 343 GGTACC GGTAC^C MboI 282 GATC ^GATC MflI 281 AGATCC [GA]^GATC[CT] MluNI 320 TGGCCA TGG^CCA MscI 320 TGGCCA TGG^CCA MseI 200 TTAA T^TAA MspI 311 CCGG C^CGG MvaI 275 CCAGG CC^[AT]GG NciI 310 CCCGG CC^[GC]GG NcoI 176 CCATGG C^CATGG NdeII 282 GATC ^GATC NspV 211 TTCGAA TT^CGAA RcaI 185 TCATGA T^CATGA Sau3AI 282 GATC ^GATC Sau96I 243 GGCCC G^G[ACGT]CC SfuI 211 TTCGAA TT^CGAA SspBI 125 TGTACA T^GTACA Tru9I 200 TTAA T^TAA TseI 303 GCTGC G^C[AT]GC Tsp509I 190 AATT ^AATT TspRI 332 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 281 AGATCC [GA]^GATC[CT] XmnI 189 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 126 GTAC GT^AC 344 GTAC BsiYI 31 CCGAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 151 CCCGATACTGG BslI 31 CCGAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 151 CCCGATACTGG BssKI 275 CCAGG ^CC[ACGT]GG 310 CCCGG BstF5I 167 CATCC GGATG(2/0) 75 GGATG FokI 167 CATCC GGATG(9/13) 75 GGATG HphI 147 TCACC GGTGA(8/7) 356 TCACC MaeIII 55 GTGAC ^GT[ACGT]AC 355 GTCAC MnlI 272 CCTC CCTC(7/6) 229 GAGG NlaIII 177 CATG CATG^ 186 CATG NlaIV 40 GGTTCC GG[ACGT]^[ACGT]CC 343 GGTACC RsaI 126 GTAC GT^AC 344 GTAC ScrFI 275 CCAGG CC^[ACGT]GG 310 CCCGG TaqI 212 TCGA T^CGA 262 TCGA Tsp45I 55 GTGAC ^GT[GC]AC 355 GTCAC TthHB8I 212 TCGA T^CGA 262 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BsaJI Cac8I CfoI EcoT14I HaeIII HhaI HinP1I MboII MwoI SfaNI StyI