################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccIII AcsI AfaI AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfaI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeIII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI TseI Tsp509I TspRI Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AclI 193 AACGTT AA^CGTT AflII 175 CTTAAG C^TTAAG AspI 205 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 164 CTCGAG C^[CT]CG[GA]G BcnI 69 CCGGG CC^[GC]GG BfrI 175 CTTAAG C^TTAAG BlpI 46 GCTAAGC GC^T[ACGT]AGC Bpu1102I 46 GCTAAGC GC^T[ACGT]AGC BsaWI 142 TCCGGT [AT]^CCGG[AT] BseRI 157 GAGGAG GAGGAG(10/8) BsiYI 54 CCATCGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 54 CCATCGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 203 GAGACG CGTCTC(1/5) BsoBI 164 CTCGAG C^[CT]CG[GA]G BsrFI 96 ACCGGC [GA]^CCGG[CT] BstNI 81 CCAGG CC^[AT]GG Cac8I 225 GCACGC GC[ACGT]^[ACGT]GC CelII 46 GCTAAGC GC^T[ACGT]AGC Cfr10I 96 ACCGGC [GA]^CCGG[CT] DsaI 251 CCATGG C^C[GA][CT]GG EcoRII 81 CCAGG ^CC[AT]GG EcoT14I 251 CCATGG C^C[AT][AT]GG HaeII 50 AGCGCC [GA]GCGC^[CT] HinfI 162 GACTC G^A[ACGT]TC HphI 212 TCACC GGTGA(8/7) MseI 176 TTAA T^TAA MslI 246 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 81 CCAGG CC^[AT]GG NciI 69 CCGGG CC^[GC]GG NcoI 251 CCATGG C^CATGG NlaIII 252 CATG CATG^ PaeR7I 164 CTCGAG C^TCGAG PflFI 205 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 162 GACTC GAGTC(4/5) Psp1406I 193 AACGTT AA^CGTT StyI 251 CCATGG C^C[AT][AT]GG Tru9I 176 TTAA T^TAA Tth111I 205 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 164 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 10 CGCG CG^CG 121 CGCG AcyI 127 GGCGTC G[GA]^CG[CT]C 205 GACGCC AspHI 64 GTGCTC G[AT]GC[AT]^C 223 GAGCAC BmyI 64 GTGCTC G[AGT]GC[ACT]^C 223 GAGCAC BsaHI 127 GGCGTC G[GA]^CG[CT]C 205 GACGCC BsaJI 81 CCAGGG C^C[ACGT][ACGT]GG 251 CCATGG BsiHKAI 64 GTGCTC G[AT]GC[AT]^C 223 GAGCAC BsmAI 160 GAGAC GTCTC(1/5) 203 GAGAC Bsp1286I 64 GTGCTC G[AGT]GC[ACT]^C 223 GAGCAC BssKI 69 CCGGG ^CC[ACGT]GG 81 CCAGG BstF5I 78 CATCC GGATG(2/0) 255 GGATG BstUI 10 CGCG CG^CG 121 CGCG DdeI 47 CTAAG C^T[ACGT]AG 186 CTTAG Fnu4HI 11 GCGGC GC^[ACGT]GC 305 GCGGC FokI 78 CATCC GGATG(9/13) 255 GGATG Hin1I 127 GGCGTC G[GA]^CG[CT]C 205 GACGCC ItaI 11 GCGGC GC^[ACGT]GC 305 GCGGC MaeII 194 ACGT A^CGT 276 ACGT MaeIII 211 GTCAC ^GT[ACGT]AC 244 GTCAC MnlI 157 GAGG CCTC(7/6) 167 GAGG MvnI 10 CGCG CG^CG 121 CGCG ScrFI 69 CCGGG CC^[ACGT]GG 81 CCAGG SmlI 164 CTCGAG C^T[CT][GA]AG 175 CTTAAG TaqI 165 TCGA T^CGA 201 TCGA Tsp45I 211 GTCAC ^GT[GC]AC 244 GTCAC TthHB8I 165 TCGA T^CGA 201 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AciI CfoI HapII HgaI HhaI HinP1I HpaII MspI MwoI