(Circular) (Six-base) MAPSORT of: pFN21AE2715.seq Check: 5994 from: 1 to: 6196 pFN21AE2715 6196 bp With 182 enzymes: SgfI * February 13, 2018 16:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 3052 3539 1937 Size: 72 1043 487 4594 Fragments arranged by size: 4594 1043 487 72 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 4323 4696 1563 Size: 2760 373 3063 Fragments arranged by size: 3063 2760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4259 5643 5911 1632 Size: 2627 1384 268 1917 Fragments arranged by size: 2627 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 5095 6072 1203 Size: 3892 977 1327 Fragments arranged by size: 3892 1327 977 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 2021 4925 2021 Size: 2904 3292 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3324 1184 Size: 2140 4056 AlwNI CAG_nnn'CTG Cuts at: 3228 3407 3939 5511 3228 Size: 179 532 1572 3913 Fragments arranged by size: 3913 1572 532 179 ApaLI G'TGCA_C Cuts at: 4253 5409 4253 Size: 1156 5040 ApoI r'AATT_y Cuts at: 1493 2288 3505 3731 3883 5992 1493 Size: 795 1217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 795 226 152 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 3521 1870 Size: 73 1578 4545 Fragments arranged by size: 4545 1578 73 BamHI G'GATC_C Cuts at: 2997 3526 3893 2997 Size: 529 367 5300 Fragments arranged by size: 5300 529 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3517 4972 618 Size: 325 217 627 1730 1455 1842 Fragments arranged by size: 1842 1730 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2581 3244 3515 1391 Size: 1190 663 271 4072 Fragments arranged by size: 4072 1190 663 271 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2689 3201 3382 928 Size: 971 790 512 181 3742 Fragments arranged by size: 3742 971 790 512 181 BbvCI CC'TCA_GC Cuts at: 2345 2345 Size: 6196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4088 5298 4088 Size: 1210 4986 BclI T'GATC_A Cuts at: 1548 2868 1548 Size: 1320 4876 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4807 136 Size: 107 122 71 631 605 3135 1525 Fragments arranged by size: 3135 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6191 6191 Size: 6196 BlpI GC'TnA_GC Cuts at: 2823 3613 2823 Size: 790 5406 Bme1580I G_kGCm'C Cuts at: 1790 4257 5413 1790 Size: 2467 1156 2573 Fragments arranged by size: 2573 2467 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2379 3475 3927 Size: 416 53 83 399 24 962 1096 452 Cuts at: 3927 4885 442 Size: 958 1753 Fragments arranged by size: 1753 1096 962 958 452 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 6196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2338 3058 3342 4856 1239 Size: 832 267 720 284 1514 2579 Fragments arranged by size: 2579 1514 832 720 284 267 Bpu10I CC'TnA_GC Cuts at: 2345 2345 Size: 6196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2058 2194 4321 5186 5484 2058 Size: 136 2127 865 298 2770 Fragments arranged by size: 2770 2127 865 298 136 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2669 2853 4859 882 Size: 617 1170 184 2006 2219 Fragments arranged by size: 2219 2006 1170 617 184 BsaAI yAC'GTr Cuts at: 493 1204 3987 493 Size: 711 2783 2702 Fragments arranged by size: 2783 2702 711 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4003 4385 5020 275 Size: 53 83 186 936 2470 382 635 1451 Fragments arranged by size: 2470 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2012 2511 4629 5027 5301 5448 5859 2012 Size: 499 2118 398 274 147 411 2349 Fragments arranged by size: 2349 2118 499 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3231 3261 1178 Size: 30 165 30 1828 30 4113 Fragments arranged by size: 4113 1828 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2657 3258 1186 Size: 1471 601 4124 Fragments arranged by size: 4124 1471 601 BseYI C'CCAG_C Cuts at: 2188 2632 5399 5895 2188 Size: 444 2767 496 2489 Fragments arranged by size: 2767 2489 496 444 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3479 1163 Size: 573 1743 3880 Fragments arranged by size: 3880 1743 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3646 4407 5435 1540 Size: 275 186 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4257 4342 5413 4257 Size: 85 1071 5040 Fragments arranged by size: 5040 1071 85 BsmI GAATG_Cn' Cuts at: 2087 3707 3800 2087 Size: 1620 93 4483 Fragments arranged by size: 4483 1620 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2581 3244 3515 4257 4342 5413 1391 Size: 399 791 663 271 742 85 1071 2174 Fragments arranged by size: 2174 1071 791 742 663 399 271 85 BspEI T'CCGG_A Cuts at: 2511 5859 2511 Size: 3348 2848 BspHI T'CATG_A Cuts at: 4085 5815 4085 Size: 1730 4466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3551 844 Size: 2707 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4083 1394 Size: 429 2260 3507 Fragments arranged by size: 3507 2260 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2178 2304 2610 4691 4865 65 Size: 1183 930 126 306 2081 174 1396 Fragments arranged by size: 2081 1396 1183 930 306 174 126 BsrFI r'CCGG_y Cuts at: 1700 1810 4840 5027 1700 Size: 110 3030 187 2869 Fragments arranged by size: 3030 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2518 96 Size: 2422 3774 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 3149 4256 5268 3149 Size: 1107 1012 4077 Fragments arranged by size: 4077 1107 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2483 794 Size: 1689 4507 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6196 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2422 Size: 6196 BstYI r'GATC_y Cuts at: 1972 2997 3170 3526 3893 4278 4295 5747 5833 Size: 1025 173 356 367 385 17 1452 86 Cuts at: 5833 5845 6191 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1025 385 367 356 346 173 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5644 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 3712 4506 4526 3712 Size: 794 20 5382 Fragments arranged by size: 5382 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6161 DraI TTT'AAA Cuts at: 2064 2293 3500 2064 Size: 229 1207 4760 Fragments arranged by size: 4760 1207 229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3445 3643 4532 Size: 54 1425 50 275 18 1615 198 889 Cuts at: 4532 6161 8 Size: 1629 43 Fragments arranged by size: 1629 1615 1425 889 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2468 2792 2856 3116 3137 4126 1353 Size: 1115 324 64 260 21 989 3423 Fragments arranged by size: 3423 1115 989 324 260 64 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2338 3058 3342 4259 4856 5643 Size: 393 439 267 720 284 917 597 787 Cuts at: 5643 5911 1239 Size: 268 1524 Fragments arranged by size: 1524 917 787 720 597 439 393 284 268 267 EcoO109I rG'GnC_Cy Cuts at: 2637 2637 Size: 6196 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 5343 1678 Size: 3665 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2007 2039 3339 3371 4837 Size: 32 135 32 435 32 1300 32 1466 Cuts at: 4837 4869 4911 4943 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1466 1300 435 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2600 3250 3412 3540 3786 669 Size: 988 281 662 650 162 128 246 3079 Fragments arranged by size: 3079 988 662 650 281 246 162 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 6196 HpaI GTT'AAC Cuts at: 3250 3412 3786 3250 Size: 162 374 5660 Fragments arranged by size: 5660 374 162 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2134 3004 3359 5310 5494 1305 Size: 829 870 355 1951 184 2007 Fragments arranged by size: 2007 1951 870 829 355 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4374 Fragments arranged by size: 4374 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3188 4154 4513 4672 518 Size: 648 2022 966 359 159 2042 Fragments arranged by size: 2042 2022 966 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3404 3612 4289 5437 5682 1606 Size: 1798 208 677 1148 245 2120 Fragments arranged by size: 2120 1798 1148 677 245 208 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5644 NdeI CA'TA_TG Cuts at: 387 2311 387 Size: 1924 4272 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 3554 5099 1132 Size: 97 2325 1545 2229 Fragments arranged by size: 2325 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2260 3162 1348 Size: 912 902 4382 Fragments arranged by size: 4382 912 902 PciI A'CATG_T Cuts at: 5095 5095 Size: 6196 PflMI CCAn_nnn'nTGG Cuts at: 1108 2539 2685 1108 Size: 1431 146 4619 Fragments arranged by size: 4619 1431 146 PfoI T'CCnGG_A Cuts at: 2087 2087 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2637 2637 Size: 6196 PshAI GACnn'nnGTC Cuts at: 1613 2319 1613 Size: 706 5490 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PstI C_TGCA'G Cuts at: 830 3548 830 Size: 2718 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 PvuII CAG'CTG Cuts at: 3404 3404 Size: 6196 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 4444 6156 4444 Size: 1712 4484 SfcI C'TryA_G Cuts at: 826 1045 2031 3544 4679 5360 5551 826 Size: 219 986 1513 1135 681 191 1471 Fragments arranged by size: 1513 1471 1135 986 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 3523 1872 Size: 1651 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2073 2173 4300 5201 5463 820 Size: 197 926 130 100 2127 901 262 1553 Fragments arranged by size: 2127 1553 926 901 262 197 130 100 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 2406 4120 5 Size: 47 2354 1714 2081 Fragments arranged by size: 2354 2081 1714 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6196 StyI C'CwwG_G Cuts at: 513 1065 3043 3635 513 Size: 552 1978 592 3074 Fragments arranged by size: 3074 1978 592 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 3503 4223 4240 4393 4578 5023 Size: 1255 594 1329 720 17 153 185 445 Cuts at: 5023 325 Size: 1498 Fragments arranged by size: 1498 1329 1255 720 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2518 4442 6154 Size: 274 80 33 51 1443 541 1924 1712 Cuts at: 6154 96 Size: 138 Fragments arranged by size: 1924 1712 1443 541 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6156 6188 6156 Size: 32 6164 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 3532 3532 Size: 6196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2392 3196 2191 Size: 201 804 5191 Fragments arranged by size: 5191 804 201 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 3521 1870 Size: 1651 4545 XmnI GAAnn'nnTTC Cuts at: 3110 4325 3110 Size: 1215 4981 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI