################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AvaII AvrII BalI BamHI BbsI BbvCI BciVI BclI BfrI BlnI BpuAI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BspMI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraIII Eco47III EcoNI EcoO65I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PpuMI PshBI Psp1406I PvuI RsrII SacI SalI SapI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI TaqI TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AhdI 448 GACGGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1102 GTGCAC G^TGCAC AocI 736 CCTGAGG CC^T[ACGT]AGG ApaI 67 GGGCCC GGGCC^C ApaLI 1102 GTGCAC G^TGCAC AspEI 448 GACGGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1102 GTGCAC G[AT]GC[AT]^C AspI 637 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1109 TGCGCA TGC^GCA BbrPI 1105 CACGTG CAC^GTG BfaI 938 CTAG C^TAG BglI 1034 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 245 AGATCT A^GATCT BlpI 984 GCTGAGC GC^T[ACGT]AGC Bpu1102I 984 GCTGAGC GC^T[ACGT]AGC BsaAI 1105 CACGTG [CT]AC^GT[GA] BsaI 1188 GGTCTC GGTCTC(1/5) BsiHKAI 1102 GTGCAC G[AT]GC[AT]^C BsmBI 1046 GAGACG CGTCTC(1/5) Bsp1407I 416 TGTACA T^GTACA BspHI 644 TCATGA T^CATGA BsrBI 541 CCGCTC CCGCTC(-3/-3) BsrDI 1290 CATTGC GCAATG(2/0) BsrGI 416 TGTACA T^GTACA BssHII 605 GCGCGC G^CGCGC Bsu36I 736 CCTGAGG CC^T[ACGT]AGG CelII 984 GCTGAGC GC^T[ACGT]AGC DraI 799 TTTAAA TTT^AAA DraII 67 GGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 1142 GACAACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 101 CCGCGG C^C[GA][CT]GG Eam1105I 448 GACGGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 400 CTCTTC CTCTTC(1/4) Eco81I 736 CCTGAGG CC^T[ACGT]AGG EcoO109I 67 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 763 GAATTC G^AATTC FspI 1109 TGCGCA TGC^GCA Ksp632I 400 CTCTTC CTCTTC(1/4) KspI 101 CCGCGG CCGC^GG MaeI 938 CTAG C^TAG MseI 800 TTAA T^TAA NaeI 1133 GCCGGC GCC^GGC NgoMIV 1133 GCCGGC G^CCGGC NotI 103 GCGGCCGC GC^GGCCGC PflFI 637 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1105 CACGTG CAC^GTG PmlI 1105 CACGTG CAC^GTG PshAI 383 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 644 TCATGA T^CATGA SacII 101 CCGCGG CCGC^GG SbfI 1232 CCTGCAGG CCTGCA^GG SmlI 211 CTCAAG C^T[CT][GA]AG Sse8387I 1232 CCTGCAGG CCTGCA^GG SspBI 416 TGTACA T^GTACA StuI 1032 AGGCCT AGG^CCT TfiI 732 GATTC G^A[AT]TC Tru9I 800 TTAA T^TAA Tth111I 637 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 763 GAATTC [GA]^AATT[CT] 919 GAATTT ApoI 763 GAATTC [GA]^AATT[CT] 919 GAATTT BanI 529 GGCGCC G^G[CT][GA]CC 1136 GGCGCC BbeI 529 GGCGCC GGCGC^C 1136 GGCGCC BcgI 351 GCAGCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 493 GCAGCCAGCTCG BpmI 1128 CTCCAG CTGGAG(16/14) 1191 CTCCAG BseRI 186 CTCCTC GAGGAG(10/8) 331 GAGGAG BsiEI 104 CGGCCG CG[GA][CT]^CG 150 CGGCCG BsmI 1330 GAATGC GAATGC(1/-1) 1359 GAATGC BstAPI 56 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 348 GCAGCAGCTGC EagI 104 CGGCCG C^GGCCG 150 CGGCCG EclXI 104 CGGCCG C^GGCCG 150 CGGCCG Eco52I 104 CGGCCG C^GGCCG 150 CGGCCG EcoT14I 1298 CCAAGG C^C[AT][AT]GG 1388 CCTTGG KasI 529 GGCGCC G^GCGCC 1136 GGCGCC MslI 639 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1354 CACATGAATG NarI 529 GGCGCC GG^CGCC 1136 GGCGCC PflMI 1173 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1263 CCAAGACCTGG SexAI 659 ACCTGGT A^CC[AT]GGT 1117 ACCAGGT SfoI 529 GGCGCC GGC^GCC 1136 GGCGCC SmaI 376 CCCGGG CCC^GGG 525 CCCGGG StyI 1298 CCAAGG C^C[AT][AT]GG 1388 CCTTGG Van91I 1173 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1263 CCAAGACCTGG XmaCI 376 CCCGGG C^CCGGG 525 CCCGGG XmaI 376 CCCGGG C^CCGGG 525 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI BanII BbvI BcnI BmyI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I Tsp509I TspRI XhoII