(Circular) (Six-base) MAPSORT of: pFN21AE2049.seq Check: 7472 from: 1 to: 6718 pFN21AE2049 6718 bp With 182 enzymes: SgfI * June 3, 2018 17:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2607 4528 278 Size: 53 83 186 936 1071 1921 2468 Fragments arranged by size: 2468 1921 1071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4061 1937 Size: 2124 4594 Acc65I G'GTAC_C Cuts at: 4039 4039 Size: 6718 AclI AA'CG_TT Cuts at: 1563 4017 4845 5218 1563 Size: 2454 828 373 3063 Fragments arranged by size: 3063 2454 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2441 4781 6165 6433 1632 Size: 809 2340 1384 268 1917 Fragments arranged by size: 2340 1917 1384 809 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6521 AflIII A'CryG_T Cuts at: 1203 2016 5617 6594 1203 Size: 813 3601 977 1327 Fragments arranged by size: 3601 1327 977 813 AgeI A'CCGG_T Cuts at: 5549 5549 Size: 6718 AhdI GACnn_n'nnGTC Cuts at: 3245 5447 3245 Size: 2202 4516 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6718 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2321 1184 Size: 1137 5581 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3442 3474 3442 Size: 32 6686 AlwNI CAG_nnn'CTG Cuts at: 3521 4461 6033 3521 Size: 940 1572 4206 Fragments arranged by size: 4206 1572 940 ApaI G_GGCC'C Cuts at: 2157 2157 Size: 6718 ApaLI G'TGCA_C Cuts at: 3350 4775 5931 3350 Size: 1425 1156 4137 Fragments arranged by size: 4137 1425 1156 ApoI r'AATT_y Cuts at: 1493 2855 3004 3286 4027 4253 4405 6514 1493 Size: 1362 149 282 741 226 152 2109 1697 Fragments arranged by size: 2109 1697 1362 741 282 226 152 149 AseI AT'TA_AT Cuts at: 160 1032 5272 160 Size: 872 4240 1606 Fragments arranged by size: 4240 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6718 AvaI C'yCGr_G Cuts at: 1870 1943 2588 4043 1870 Size: 73 645 1455 4545 Fragments arranged by size: 4545 1455 645 73 BamHI G'GATC_C Cuts at: 3442 4048 4415 3442 Size: 606 367 5745 Fragments arranged by size: 5745 606 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2546 2567 2584 4039 5494 Size: 325 217 627 759 21 17 1455 1455 Cuts at: 5494 618 Size: 1842 Fragments arranged by size: 1842 1455 1455 759 627 325 217 21 17 BanII G_rGCy'C Cuts at: 1391 2157 3120 3369 4037 1391 Size: 766 963 249 668 4072 Fragments arranged by size: 4072 963 766 668 249 BbeI G_GCGC'C Cuts at: 2588 2588 Size: 6718 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5747 BbvCI CC'TCA_GC Cuts at: 2531 2531 Size: 6718 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4909 4943 1903 Size: 34 50 34 2888 34 3678 Fragments arranged by size: 3678 2888 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4610 5820 4610 Size: 1210 5508 BclI T'GATC_A Cuts at: 1548 2130 1548 Size: 582 6136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3364 5329 136 Size: 107 122 71 631 605 1692 1965 1525 Fragments arranged by size: 1965 1692 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6713 6713 Size: 6718 BlpI GC'TnA_GC Cuts at: 4135 4135 Size: 6718 Bme1580I G_kGCm'C Cuts at: 1790 2157 3354 3811 4779 5935 1790 Size: 367 1197 457 968 1156 2573 Fragments arranged by size: 2573 1197 1156 968 457 367 BmgBI CAC'GTC Cuts at: 1318 2905 1318 Size: 1587 5131 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3509 3827 4449 Size: 416 53 83 399 24 2092 318 622 Cuts at: 4449 5407 442 Size: 958 1753 Fragments arranged by size: 2092 1753 958 622 416 399 318 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 3113 5378 1239 Size: 839 1035 2265 2579 Fragments arranged by size: 2579 2265 1035 839 Bpu10I CC'TnA_GC Cuts at: 2531 2531 Size: 6718 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3366 4843 5708 6006 3366 Size: 1477 865 298 4078 Fragments arranged by size: 4078 1477 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3253 3518 5381 882 Size: 617 1754 265 1863 2219 Fragments arranged by size: 2219 1863 1754 617 265 BsaAI yAC'GTr Cuts at: 493 1204 2311 4509 493 Size: 711 1107 2198 2702 Fragments arranged by size: 2702 2198 1107 711 BsaBI GATnn'nnATC Cuts at: 6712 6712 Size: 6718 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2244 2585 2604 4525 Size: 53 83 186 936 711 341 19 1921 Cuts at: 4525 4907 5542 275 Size: 382 635 1451 Fragments arranged by size: 1921 1451 936 711 635 382 341 186 83 53 19 BsaWI w'CCGG_w Cuts at: 2818 5151 5549 5823 5970 6381 2818 Size: 2333 398 274 147 411 3155 Fragments arranged by size: 3155 2333 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3062 3092 3924 3954 1178 Size: 30 165 30 1659 30 832 30 3942 Fragments arranged by size: 3942 1659 832 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 3083 3242 1186 Size: 1018 879 159 4662 Fragments arranged by size: 4662 1018 879 159 BseYI C'CCAG_C Cuts at: 2805 3119 5921 6417 2805 Size: 314 2802 496 3106 Fragments arranged by size: 3106 2802 496 314 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2085 3017 3348 1163 Size: 573 349 932 331 4533 Fragments arranged by size: 4533 932 573 349 331 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2831 4168 4929 5957 1540 Size: 275 186 830 1337 761 1028 2301 Fragments arranged by size: 2301 1337 1028 830 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2523 2595 3099 3354 4779 4864 5935 2172 Size: 351 72 504 255 1425 85 1071 2955 Fragments arranged by size: 2955 1425 1071 504 351 255 85 72 BsmI GAATG_Cn' Cuts at: 2961 3618 4229 4322 2961 Size: 657 611 93 5357 Fragments arranged by size: 5357 657 611 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3232 3232 Size: 6718 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2172 2523 2595 3099 3120 3354 Size: 399 367 15 351 72 504 21 234 Cuts at: 3354 3369 3811 4037 4779 4864 5935 1391 Size: 15 442 226 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 442 399 367 351 234 226 85 72 21 15 15 BspEI T'CCGG_A Cuts at: 2818 6381 2818 Size: 3563 3155 BspHI T'CATG_A Cuts at: 2127 2133 2793 4607 6337 2127 Size: 6 660 1814 1730 2508 Fragments arranged by size: 2508 1814 1730 660 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 4073 844 Size: 2602 627 3489 Fragments arranged by size: 3489 2602 627 BsrBI CCG'CTC Cuts at: 1394 1823 4605 1394 Size: 429 2782 3507 Fragments arranged by size: 3507 2782 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5213 5387 65 Size: 1183 3965 174 1396 Fragments arranged by size: 3965 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2997 5362 5549 1700 Size: 110 1187 2365 187 2869 Fragments arranged by size: 2869 2365 1187 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 2637 96 Size: 2049 492 4177 Fragments arranged by size: 4177 2049 492 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6718 BssSI C'ACGA_G Cuts at: 4778 5790 4778 Size: 1012 5706 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6718 BstBI TT'CG_AA Cuts at: 5522 5522 Size: 6718 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6718 BstXI CCAn_nnnn'nTGG Cuts at: 3050 3164 3050 Size: 114 6604 BstYI r'GATC_y Cuts at: 1972 3442 4048 4415 4800 4817 6269 6355 6367 Size: 1470 606 367 385 17 1452 86 12 Cuts at: 6367 6713 1972 Size: 346 1977 Fragments arranged by size: 1977 1470 1452 606 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3361 3361 Size: 6718 BtgI C'CryG_G Cuts at: 513 1065 3936 513 Size: 552 2871 3295 Fragments arranged by size: 3295 2871 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5979 Fragments arranged by size: 5979 683 56 BtsI GCAGTG_nn' Cuts at: 3029 3312 3354 4234 5028 5048 3029 Size: 283 42 880 794 20 4699 Fragments arranged by size: 4699 880 794 283 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6718 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6683 DraI TTT'AAA Cuts at: 2916 4022 2916 Size: 1106 5612 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5725 809 Size: 507 4409 1802 Fragments arranged by size: 4409 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2828 4165 5054 Size: 54 1425 50 275 18 998 1337 889 Cuts at: 5054 6683 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1337 998 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2828 4165 1537 Size: 275 1016 1337 4090 Fragments arranged by size: 4090 1337 1016 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2511 2892 2903 2945 2970 3102 3696 4648 Size: 1158 381 11 42 25 132 594 952 Cuts at: 4648 1353 Size: 3423 Fragments arranged by size: 3423 1158 952 594 381 132 42 25 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5305 5679 5825 387 Size: 1064 138 3716 374 146 1280 Fragments arranged by size: 3716 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2441 3113 4781 5378 6165 6433 Size: 393 446 363 672 1668 597 787 268 Cuts at: 6433 1239 Size: 1524 Fragments arranged by size: 1668 1524 787 672 597 446 393 363 268 EcoNI CCTnn'n_nnAGG Cuts at: 2186 2186 Size: 6718 EcoO109I rG'GnC_Cy Cuts at: 2153 2179 2416 3059 3458 2153 Size: 26 237 643 399 5413 Fragments arranged by size: 5413 643 399 237 26 EcoRI G'AATT_C Cuts at: 4027 4405 4027 Size: 378 6340 FspI TGC'GCA Cuts at: 5224 5224 Size: 6718 HaeII r_GCGC'y Cuts at: 1678 2588 3962 5865 1678 Size: 910 1374 1903 2531 Fragments arranged by size: 2531 1903 1374 910 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 2317 2349 2769 Size: 32 135 32 543 32 170 32 420 Cuts at: 2769 2801 3231 3263 3517 3549 5359 5391 5433 Size: 32 430 32 254 32 1810 32 42 Cuts at: 5433 5465 1373 Size: 32 2626 Fragments arranged by size: 2626 1810 543 430 420 254 170 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2608 4062 4308 669 Size: 988 281 670 1454 246 3079 Fragments arranged by size: 3079 1454 988 670 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6718 HpaI GTT'AAC Cuts at: 4308 4308 Size: 6718 KasI G'GCGC_C Cuts at: 2584 2584 Size: 6718 KpnI G_GTAC'C Cuts at: 4043 4043 Size: 6718 MfeI C'AATT_G Cuts at: 4317 4317 Size: 6718 MluI A'CGCG_T Cuts at: 6594 6594 Size: 6718 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2610 5832 6016 1305 Size: 1305 3222 184 2007 Fragments arranged by size: 3222 2007 1305 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4896 Fragments arranged by size: 4896 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2132 2598 3162 3342 3858 4676 Size: 648 870 96 466 564 180 516 818 Cuts at: 4676 5035 5194 518 Size: 359 159 2042 Fragments arranged by size: 2042 870 818 648 564 516 466 359 180 159 96 MspA1I CmG'CkG Cuts at: 1606 2079 3710 4134 4811 5959 6204 1606 Size: 473 1631 424 677 1148 245 2120 Fragments arranged by size: 2120 1631 1148 677 473 424 245 NarI GG'CG_CC Cuts at: 2585 2585 Size: 6718 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6166 NdeI CA'TA_TG Cuts at: 387 2686 3492 387 Size: 2299 806 3613 Fragments arranged by size: 3613 2299 806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6718 NotI GC'GGCC_GC Cuts at: 4165 4165 Size: 6718 NspI r_CATG'y Cuts at: 1132 1229 2020 4076 5621 1132 Size: 97 791 2056 1545 2229 Fragments arranged by size: 2229 2056 1545 791 97 PasI CC'CwG_GG Cuts at: 1348 2119 3043 1348 Size: 771 924 5023 Fragments arranged by size: 5023 924 771 PciI A'CATG_T Cuts at: 2016 5617 2016 Size: 3601 3117 PflMI CCAn_nnn'nTGG Cuts at: 1108 3468 1108 Size: 2360 4358 PfoI T'CCnGG_A Cuts at: 2055 3445 3974 2055 Size: 1390 529 4799 Fragments arranged by size: 4799 1390 529 PmeI GTTT'AAAC Cuts at: 4022 4022 Size: 6718 PmlI CAC'GTG Cuts at: 2311 2311 Size: 6718 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4787 4819 6300 6332 4787 Size: 32 1481 32 5173 Fragments arranged by size: 5173 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2416 3059 3458 2416 Size: 643 399 5676 Fragments arranged by size: 5676 643 399 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6718 PsiI TTA'TAA Cuts at: 4288 4288 Size: 6718 PspOMI G'GGCC_C Cuts at: 2153 2153 Size: 6718 PstI C_TGCA'G Cuts at: 830 3129 4070 830 Size: 2299 941 3478 Fragments arranged by size: 3478 2299 941 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6718 PvuII CAG'CTG Cuts at: 3710 3710 Size: 6718 SalI G'TCGA_C Cuts at: 1936 4060 1936 Size: 2124 4594 SapI GCTCTTCn'nnn_ Cuts at: 2511 3696 2511 Size: 1185 5533 SbfI CC_TGCA'GG Cuts at: 4070 4070 Size: 6718 ScaI AGT'ACT Cuts at: 2267 2332 2655 2768 3387 3613 4966 6678 2267 Size: 65 323 113 619 226 1353 1712 2307 Fragments arranged by size: 2307 1712 1353 619 323 226 113 65 SfcI C'TryA_G Cuts at: 826 1045 2649 3125 4066 5201 5882 6073 826 Size: 219 1604 476 941 1135 681 191 1471 Fragments arranged by size: 1604 1471 1135 941 681 476 219 191 SfoI GGC'GCC Cuts at: 2586 2586 Size: 6718 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6718 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6718 SmaI CCC'GGG Cuts at: 1872 4045 1872 Size: 2173 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3381 4822 5723 5985 820 Size: 197 926 1438 1441 901 262 1553 Fragments arranged by size: 1553 1441 1438 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4509 493 Size: 4016 2702 SpeI A'CTAG_T Cuts at: 152 3743 152 Size: 3591 3127 SphI G_CATG'C Cuts at: 1132 4076 1132 Size: 2944 3774 SspI AAT'ATT Cuts at: 5 52 4642 5 Size: 47 4590 2081 Fragments arranged by size: 4590 2081 47 StuI AGG'CCT Cuts at: 1517 2863 1517 Size: 1346 5372 StyI C'CwwG_G Cuts at: 513 1065 4157 513 Size: 552 3092 3074 Fragments arranged by size: 3092 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3114 3562 3898 4745 4762 4915 5100 Size: 1255 1534 448 336 847 17 153 185 Cuts at: 5100 5545 325 Size: 445 1498 Fragments arranged by size: 1534 1498 1255 847 448 445 336 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 2265 2283 Size: 274 80 33 51 1443 168 120 18 Cuts at: 2283 2330 2637 2653 2766 3385 3611 4964 6676 Size: 47 307 16 113 619 226 1353 1712 Cuts at: 6676 96 Size: 138 Fragments arranged by size: 1712 1443 1353 619 307 274 226 168 138 120 113 80 51 47 33 18 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 3790 3822 3924 3956 3988 6678 6710 Size: 32 1370 32 102 32 32 2690 32 Cuts at: 6710 2388 Size: 2396 Fragments arranged by size: 2690 2396 1370 102 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6718 XbaI T'CTAG_A Cuts at: 4054 4054 Size: 6718 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 3208 2053 Size: 1155 5563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6718 XmaI C'CCGG_G Cuts at: 1870 4043 1870 Size: 2173 4545 XmnI GAAnn'nnTTC Cuts at: 4847 4847 Size: 6718 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2605 4526 276 Size: 53 83 186 936 1071 1921 2468 Fragments arranged by size: 2468 1921 1071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI