################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BglII BpmI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI HphI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccIII 432 TCCGGA T^CCGGA AflII 634 CTTAAG C^TTAAG AlwI 882 GGATC GGATC(4/5) AlwNI 211 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1097 CCTCAGG CC^T[ACGT]AGG Asp700I 190 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 870 CCTAGG C^CTAGG BbsI 387 GAAGAC GAAGAC(2/6) BfrI 634 CTTAAG C^TTAAG BlnI 870 CCTAGG C^CTAGG BpuAI 387 GAAGAC GAAGAC(2/6) BsaWI 432 TCCGGA [AT]^CCGG[AT] BseAI 432 TCCGGA T^CCGGA BseRI 670 GAGGAG GAGGAG(10/8) BsgI 877 GTGCAG GTGCAG(16/14) BsmFI 133 GGGAC GGGAC(10/14) BspEI 432 TCCGGA T^CCGGA BspHI 524 TCATGA T^CATGA BstYI 882 GGATCT [GA]^GATC[CT] Bsu36I 1097 CCTCAGG CC^T[ACGT]AGG DraII 629 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 1097 CCTCAGG CC^T[ACGT]AGG EcoNI 658 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 629 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 226 GATATC GAT^ATC HgaI 244 GCGTC GACGC(5/10) HincII 747 GTTGAC GT[CT]^[GA]AC HindII 747 GTTGAC GT[CT]^[GA]AC MaeII 237 ACGT A^CGT MflI 882 GGATCT [GA]^GATC[CT] MroI 432 TCCGGA T^CCGGA MslI 1032 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 119 CCATGG C^CATGG PpuMI 629 AGGACCT [GA]G^G[AT]CC[CT] RcaI 524 TCATGA T^CATGA SmlI 634 CTTAAG C^T[CT][GA]AG SphI 366 GCATGC GCATG^C XhoII 882 GGATCT [GA]^GATC[CT] XmnI 190 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 12 GAGCTC G[AT]GC[AT]^C 673 GAGCTC BalI 116 TGGCCA TGG^CCA 347 TGGCCA BanII 12 GAGCTC G[GA]GC[CT]^C 673 GAGCTC BfaI 481 CTAG C^TAG 871 CTAG BmyI 12 GAGCTC G[AGT]GC[ACT]^C 673 GAGCTC BsiHKAI 12 GAGCTC G[AT]GC[AT]^C 673 GAGCTC BsmI 219 GAATGC GAATGC(1/-1) 543 GAATGC Bsp1286I 12 GAGCTC G[AGT]GC[ACT]^C 673 GAGCTC DpnII 883 GATC ^GATC 907 GATC DsaI 119 CCATGG C^C[GA][CT]GG 719 CCACGG EaeI 116 TGGCCA [CT]^GGCC[GA] 347 TGGCCA FauI 26 CCCGC CCCGC(4/6) 866 CCCGC MaeI 481 CTAG C^TAG 871 CTAG MaeIII 143 GTAAC ^GT[ACGT]AC 842 GTTAC MboI 883 GATC ^GATC 907 GATC MluNI 116 TGGCCA TGG^CCA 347 TGGCCA MscI 116 TGGCCA TGG^CCA 347 TGGCCA NdeI 332 CATATG CA^TATG 614 CATATG NdeII 883 GATC ^GATC 907 GATC NlaIV 133 GGGACC GG[ACGT]^[ACGT]CC 925 GGAGCC SacI 12 GAGCTC GAGCT^C 673 GAGCTC Sau3AI 883 GATC ^GATC 907 GATC TfiI 206 GATTC G^A[AT]TC 786 GAATC XcmI 119 CCATGGTTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 821 CCAGGTTCAAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AluI ApoI AvaII BbvI BlpI Bpu1102I BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I CelII Cfr13I DdeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MboII MnlI MseI MspA1I MspI MvaI MwoI NlaIII NspI PleI PvuII Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I