(Circular) (Six-base) MAPSORT of: pFN21AE1858.seq Check: 8266 from: 1 to: 5842 pFN21AE1858 5842 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 3163 3163 Size: 5842 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 2400 2753 3905 5289 5557 1632 Size: 454 314 353 1152 1384 268 1917 Fragments arranged by size: 1917 1384 1152 454 353 314 268 AflII C'TTAA_G Cuts at: 820 1017 2639 820 Size: 197 1622 4023 Fragments arranged by size: 4023 1622 197 AflIII A'CryG_T Cuts at: 1203 4741 5718 1203 Size: 3538 977 1327 Fragments arranged by size: 3538 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3049 3059 1184 Size: 1865 10 3967 Fragments arranged by size: 3967 1865 10 AlwNI CAG_nnn'CTG Cuts at: 2221 3585 5157 2221 Size: 1364 1572 2906 Fragments arranged by size: 2906 1572 1364 ApaLI G'TGCA_C Cuts at: 3899 5055 3899 Size: 1156 4686 ApoI r'AATT_y Cuts at: 1493 2433 2525 2612 3055 3151 3377 3529 5638 Size: 940 92 87 443 96 226 152 2109 Cuts at: 5638 1493 Size: 1697 Fragments arranged by size: 2109 1697 940 443 226 152 96 92 87 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 3167 1870 Size: 73 1224 4545 Fragments arranged by size: 4545 1224 73 AvrII C'CTAG_G Cuts at: 2875 2875 Size: 5842 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3163 4618 618 Size: 325 217 627 1376 1455 1842 Fragments arranged by size: 1842 1455 1376 627 325 217 BanII G_rGCy'C Cuts at: 1391 2021 2682 3161 1391 Size: 630 661 479 4072 Fragments arranged by size: 4072 661 630 479 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2399 928 Size: 971 500 4371 Fragments arranged by size: 4371 971 500 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 1548 Size: 5842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4453 136 Size: 107 122 71 631 605 2781 1525 Fragments arranged by size: 2781 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5837 5837 Size: 5842 BlpI GC'TnA_GC Cuts at: 2015 2020 2681 3259 2015 Size: 5 661 578 4598 Fragments arranged by size: 4598 661 578 5 Bme1580I G_kGCm'C Cuts at: 1790 3903 5059 1790 Size: 2113 1156 2573 Fragments arranged by size: 2573 2113 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3573 4531 442 Size: 416 53 83 399 24 2156 958 1753 Fragments arranged by size: 2156 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2868 2868 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4502 1239 Size: 3263 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3967 4832 5130 3967 Size: 865 298 4679 Fragments arranged by size: 4679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4505 882 Size: 617 3006 2219 Fragments arranged by size: 3006 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3633 493 Size: 711 2429 2702 Fragments arranged by size: 2702 2429 711 BsaBI GATnn'nnATC Cuts at: 5836 5836 Size: 5842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3649 4031 4666 275 Size: 53 83 186 936 2116 382 635 1451 Fragments arranged by size: 2116 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2437 4275 4673 4947 5094 5505 2437 Size: 1838 398 274 147 411 2774 Fragments arranged by size: 2774 1838 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5617 Fragments arranged by size: 5617 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 1186 Size: 1504 4338 BseYI C'CCAG_C Cuts at: 5045 5541 5045 Size: 496 5346 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2903 1163 Size: 573 1167 4102 Fragments arranged by size: 4102 1167 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 4053 5081 1540 Size: 275 186 1291 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 2682 3903 3988 5059 2021 Size: 661 1221 85 1071 2804 Fragments arranged by size: 2804 1221 1071 661 85 BsmI GAATG_Cn' Cuts at: 2230 2554 3353 3446 2230 Size: 324 799 93 4626 Fragments arranged by size: 4626 799 324 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2682 3161 3903 3988 5059 1391 Size: 399 231 661 479 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 661 479 399 231 85 BspEI T'CCGG_A Cuts at: 2437 5505 2437 Size: 3068 2774 BspHI T'CATG_A Cuts at: 2529 3731 5461 2529 Size: 1202 1730 2910 Fragments arranged by size: 2910 1730 1202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3197 844 Size: 2353 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3729 1394 Size: 429 1906 3507 Fragments arranged by size: 3507 1906 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4337 4511 65 Size: 1183 3089 174 1396 Fragments arranged by size: 3089 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4486 4673 1700 Size: 110 2676 187 2869 Fragments arranged by size: 2869 2676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 4646 4646 Size: 5842 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5842 BstYI r'GATC_y Cuts at: 1972 2887 3172 3539 3924 3941 5393 5479 5491 Size: 915 285 367 385 17 1452 86 12 Cuts at: 5491 5837 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 915 385 367 346 285 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3103 3103 Size: 5842 BtgI C'CryG_G Cuts at: 513 1065 2004 2124 2724 513 Size: 552 939 120 600 3631 Fragments arranged by size: 3631 939 600 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3007 487 Size: 683 56 1781 3322 Fragments arranged by size: 3322 1781 683 56 BtsI GCAGTG_nn' Cuts at: 3060 3358 4152 4172 3060 Size: 298 794 20 4730 Fragments arranged by size: 4730 794 298 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2224 2259 529 Size: 35 1660 35 4112 Fragments arranged by size: 4112 1660 35 35 DraI TTT'AAA Cuts at: 3146 3146 Size: 5842 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2352 3289 Size: 54 1425 50 275 18 291 231 937 Cuts at: 3289 4178 5807 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 937 889 291 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3289 1537 Size: 275 1477 4090 Fragments arranged by size: 4090 1477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2835 2982 3772 1353 Size: 837 645 147 790 3423 Fragments arranged by size: 3423 837 790 645 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4429 4803 4949 387 Size: 1064 138 2840 374 146 1280 Fragments arranged by size: 2840 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2019 2680 2019 Size: 661 5181 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2400 2753 3905 4502 5289 5557 Size: 393 454 314 353 1152 597 787 268 Cuts at: 5557 1239 Size: 1524 Fragments arranged by size: 1524 1152 787 597 454 393 353 314 268 EcoNI CCTnn'n_nnAGG Cuts at: 2667 2667 Size: 5842 EcoO109I rG'GnC_Cy Cuts at: 2635 2635 Size: 5842 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 EcoRV GAT'ATC Cuts at: 2233 2233 Size: 5842 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 4989 1678 Size: 3311 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2705 2737 4483 4515 4557 Size: 32 135 32 1133 32 1746 32 42 Cuts at: 4557 4589 1373 Size: 32 2626 Fragments arranged by size: 2626 1746 1133 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2754 3186 3432 669 Size: 988 281 816 432 246 3079 Fragments arranged by size: 3079 988 816 432 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5842 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KpnI G_GTAC'C Cuts at: 3167 3167 Size: 5842 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2886 4956 5140 1305 Size: 1581 2070 184 2007 Fragments arranged by size: 2070 2007 1581 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 2354 10 Size: 54 1425 343 291 231 3498 Fragments arranged by size: 3498 1425 343 291 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 3041 3800 4159 4318 518 Size: 648 1875 759 359 159 2042 Fragments arranged by size: 2042 1875 759 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2164 3008 3258 3935 5083 5328 1606 Size: 408 150 844 250 677 1148 245 2120 Fragments arranged by size: 2120 1148 844 677 408 250 245 150 NcoI C'CATG_G Cuts at: 513 1065 2004 2124 513 Size: 552 939 120 4231 Fragments arranged by size: 4231 939 552 120 NdeI CA'TA_TG Cuts at: 387 2338 2620 387 Size: 1951 282 3609 Fragments arranged by size: 3609 1951 282 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NspI r_CATG'y Cuts at: 1132 1229 2375 2926 3040 3200 4745 1132 Size: 97 1146 551 114 160 1545 2229 Fragments arranged by size: 2229 1545 1146 551 160 114 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5842 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2635 2635 Size: 5842 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PstI C_TGCA'G Cuts at: 830 3194 830 Size: 2364 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 PvuII CAG'CTG Cuts at: 2014 2164 3008 2014 Size: 150 844 4848 Fragments arranged by size: 4848 844 150 SacI G_AGCT'C Cuts at: 2021 2682 2021 Size: 661 5181 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 4090 5802 4090 Size: 1712 4130 SfcI C'TryA_G Cuts at: 826 1045 3190 4325 5006 5197 826 Size: 219 2145 1135 681 191 1471 Fragments arranged by size: 2145 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 3169 1872 Size: 1297 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 3946 4847 5109 820 Size: 197 926 696 1307 901 262 1553 Fragments arranged by size: 1553 1307 926 901 696 262 197 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 2375 3200 1132 Size: 1243 825 3774 Fragments arranged by size: 3774 1243 825 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5842 StyI C'CwwG_G Cuts at: 513 1065 2004 2040 2124 2668 2875 3281 513 Size: 552 939 36 84 544 207 406 3074 Fragments arranged by size: 3074 939 552 544 406 207 84 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3869 3886 4039 4224 4669 325 Size: 1255 2289 17 153 185 445 1498 Fragments arranged by size: 2289 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4088 5800 96 Size: 274 80 33 51 1443 2111 1712 138 Fragments arranged by size: 2111 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2627 2659 5802 5834 2627 Size: 32 3143 32 2635 Fragments arranged by size: 3143 2635 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 2833 2131 Size: 702 5140 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 3167 1870 Size: 1297 4545 XmnI GAAnn'nnTTC Cuts at: 2199 3971 2199 Size: 1772 4070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI