################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 571 GTAGAC GT^[AC][GT]AC AcsI 495 GAATTT [GA]^AATT[CT] AocI 780 CCTGAGG CC^T[ACGT]AGG ApaI 800 GGGCCC GGGCC^C ApoI 495 GAATTT [GA]^AATT[CT] Asp700I 525 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 441 GAGCTC G[AT]GC[AT]^C AspI 73 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 787 CCTAGG C^CTAGG BbvCI 189 CCTCAGC CCTCAGC(-5/-2) BcnI 798 CCGGG CC^[GC]GG BlnI 787 CCTAGG C^CTAGG BpmI 427 CTCCAG CTGGAG(16/14) BsiHKAI 441 GAGCTC G[AT]GC[AT]^C BsmBI 4 GAGACG CGTCTC(1/5) BspDI 463 ATCGAT AT^CGAT BspHI 407 TCATGA T^CATGA BspMI 721 ACCTGC ACCTGC(4/8) BstYI 328 GGATCT [GA]^GATC[CT] Bsu36I 780 CCTGAGG CC^T[ACGT]AGG ClaI 463 ATCGAT AT^CGAT DsaI 89 CCGTGG C^C[GA][CT]GG Eco81I 780 CCTGAGG CC^T[ACGT]AGG EcoT14I 787 CCTAGG C^C[AT][AT]GG HapII 798 CCGG C^CGG HpaII 798 CCGG C^CGG MaeII 7 ACGT A^CGT MaeIII 745 GTCAC ^GT[ACGT]AC MflI 328 GGATCT [GA]^GATC[CT] MspI 798 CCGG C^CGG NciI 798 CCGGG CC^[GC]GG PflFI 73 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 477 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 350 GACTC GAGTC(4/5) PshAI 738 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 924 CTGCAG CTGCA^G PvuII 740 CAGCTG CAG^CTG RcaI 407 TCATGA T^CATGA SacI 441 GAGCTC GAGCT^C ScaI 64 AGTACT AGT^ACT SfcI 924 CTGCAG C^T[GA][CT]AG StyI 787 CCTAGG C^C[AT][AT]GG TaqI 464 TCGA T^CGA TfiI 411 GAATC G^A[AT]TC Tsp45I 745 GTCAC ^GT[GC]AC Tth111I 73 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 464 TCGA T^CGA Van91I 477 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 275 CCATCTCCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 328 GGATCT [GA]^GATC[CT] XmnI 525 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BalI 374 TGGCCA TGG^CCA 716 TGGCCA BbsI 103 GTCTTC GAAGAC(2/6) 613 GTCTTC BfaI 667 CTAG C^TAG 788 CTAG BpuAI 103 GTCTTC GAAGAC(2/6) 613 GTCTTC BseRI 133 CTCCTC GAGGAG(10/8) 726 CTCCTC BsmAI 4 GAGAC GTCTC(1/5) 425 GTCTC BsmFI 348 GGGAC GGGAC(10/14) 670 GGGAC BsrI 436 ACTGG ACTGG(1/-1) 18 CCAGT BstF5I 402 CATCC GGATG(2/0) 578 CATCC BstXI 429 CCAGATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 638 CCATCATCGTGG DraII 710 AGGCCCT [GA]G^G[ACGT]CC[CT] 800 GGGCCCC EaeI 374 TGGCCA [CT]^GGCC[GA] 716 TGGCCA EcoO109I 710 AGGCCCT [GA]G^G[ACGT]CC[CT] 800 GGGCCCC FokI 402 CATCC GGATG(9/13) 578 CATCC HinfI 350 GACTC G^A[ACGT]TC 411 GAATC MaeI 667 CTAG C^TAG 788 CTAG MluNI 374 TGGCCA TGG^CCA 716 TGGCCA MscI 374 TGGCCA TGG^CCA 716 TGGCCA MspA1I 740 CAGCTG C[AC]G^C[GT]G 921 CCGCTG SfaNI 543 GATGC GCATC(5/9) 50 GCATC SmlI 513 CTTGAG C^T[CT][GA]AG 876 CTTGAG StuI 698 AGGCCT AGG^CCT 784 AGGCCT Tsp509I 303 AATT ^AATT 496 AATT TspRI 110 CAGTG CA[GC]TG[ACGT][ACGT]^ 902 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI HaeIII HphI ItaI Ksp632I MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI