################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BseAI BsgI BsiEI BsiWI BsiYI BslI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 1014 GTCTAC GT^[AC][GT]AC AvaI 1036 CCCGAG C^[CT]CG[GA]G BanII 1031 GAGCTC G[GA]GC[CT]^C BbsI 655 GTCTTC GAAGAC(2/6) BcgI 292 CGAATCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1019 CCCGG CC^[GC]GG BpuAI 655 GTCTTC GAAGAC(2/6) BsaBI 991 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1061 GGTCTC GGTCTC(1/5) BsaWI 206 ACCGGA [AT]^CCGG[AT] BsoBI 1036 CCCGAG C^[CT]CG[GA]G BstAPI 1085 GCATAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 433 GGTCACC G^GT[ACGT]ACC BstPI 433 GGTCACC G^GT[ACGT]ACC Cac8I 548 GCATGC GC[ACGT]^[ACGT]GC CfoI 348 GCGC GCG^C DraI 145 TTTAAA TTT^AAA DrdI 892 GACTATGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 433 GGTCACC G^GT[ACGT]ACC EcoRV 952 GATATC GAT^ATC EcoT22I 550 ATGCAT ATGCA^T FauI 284 GCGGG CCCGC(4/6) HaeIII 583 GGCC GG^CC HgaI 1286 GACGC GACGC(5/10) HhaI 348 GCGC GCG^C HinP1I 348 GCGC G^CGC MamI 991 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 694 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 1019 CCCGG CC^[GC]GG NsiI 550 ATGCAT ATGCA^T PleI 1134 GACTC GAGTC(4/5) PstI 300 CTGCAG CTGCA^G SacI 1031 GAGCTC GAGCT^C SapI 384 GAAGAGC GCTCTTC(1/4) SexAI 1349 ACCTGGT A^CC[AT]GGT SphI 548 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 822 AAATTT [GA]^AATT[CT] 1306 GAATTT ApoI 822 AAATTT [GA]^AATT[CT] 1306 GAATTT BclI 531 TGATCA T^GATCA 687 TGATCA BseRI 90 GAGGAG GAGGAG(10/8) 427 GAGGAG BsmBI 455 GAGACG CGTCTC(1/5) 1284 GAGACG BsmI 6 GCATTC GAATGC(1/-1) 1094 GCATTC BspHI 1137 TCATGA T^CATGA 1320 TCATGA BsrDI 1406 CATTGC GCAATG(2/0) 1159 GCAATG BstBI 381 TTCGAA TT^CGAA 1355 TTCGAA DraII 178 AGGTCCT [GA]G^G[ACGT]CC[CT] 1179 AGGACCC EarI 87 GAAGAG CTCTTC(1/4) 384 GAAGAG EcoO109I 178 AGGTCCT [GA]G^G[ACGT]CC[CT] 1179 AGGACCC EcoT14I 1113 CCTTGG C^C[AT][AT]GG 1394 CCTTGG FbaI 531 TGATCA T^GATCA 687 TGATCA HapII 207 CCGG C^CGG 1020 CCGG HincII 217 GTTGAC GT[CT]^[GA]AC 964 GTTGAC HindII 217 GTTGAC GT[CT]^[GA]AC 964 GTTGAC HpaII 207 CCGG C^CGG 1020 CCGG Ksp632I 87 GAAGAG CTCTTC(1/4) 384 GAAGAG MspI 207 CCGG C^CGG 1020 CCGG NspI 548 GCATGC [GA]CATG^[CT] 552 GCATGT NspV 381 TTCGAA TT^CGAA 1355 TTCGAA PpuMI 178 AGGTCCT [GA]G^G[AT]CC[CT] 1179 AGGACCC RcaI 1137 TCATGA T^CATGA 1320 TCATGA SfaNI 672 GATGC GCATC(5/9) 1413 GCATC SfcI 300 CTGCAG C^T[GA][CT]AG 1026 CTACAG SfuI 381 TTCGAA TT^CGAA 1355 TTCGAA StyI 1113 CCTTGG C^C[AT][AT]GG 1394 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BbvI BfaI BglII BmyI BsaJI BsiHKAI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScaI ScrFI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII