(Circular) (Six-base) MAPSORT of: pFN21AE0953.seq Check: 1212 from: 1 to: 6148 pFN21AE0953 6148 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 3491 1937 Size: 1083 471 4594 Fragments arranged by size: 4594 1083 471 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2301 2885 4211 5595 5863 1632 Size: 669 584 1326 1384 268 1917 Fragments arranged by size: 1917 1384 1326 669 584 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2014 2046 2014 Size: 32 6116 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 2827 3311 3457 3683 3835 5944 1493 Size: 1334 484 146 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 484 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 3041 3473 1870 Size: 73 1098 432 4545 Fragments arranged by size: 4545 1098 432 73 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3469 4924 618 Size: 325 217 627 1682 1455 1842 Fragments arranged by size: 1842 1682 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3040 3467 1391 Size: 1649 427 4072 Fragments arranged by size: 4072 1649 427 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2653 928 Size: 971 754 4423 Fragments arranged by size: 4423 971 754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2286 2320 4339 4373 1903 Size: 34 50 34 265 34 2019 34 3678 Fragments arranged by size: 3678 2019 265 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 2536 2692 1548 Size: 988 156 5004 Fragments arranged by size: 5004 988 156 BfrBI ATG'CAT Cuts at: 2557 2557 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2099 2811 2860 6143 2099 Size: 712 49 3283 2104 Fragments arranged by size: 3283 2104 712 49 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 4209 5365 1790 Size: 2419 1156 2573 Fragments arranged by size: 2573 2419 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2738 3879 4837 Size: 416 53 83 399 24 1321 1141 958 Cuts at: 4837 442 Size: 1753 Fragments arranged by size: 1753 1321 1141 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2118 2118 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4808 1239 Size: 3569 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4273 5138 5436 4273 Size: 865 298 4985 Fragments arranged by size: 4985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3072 4811 882 Size: 617 1573 1739 2219 Fragments arranged by size: 2219 1739 1573 617 BsaAI yAC'GTr Cuts at: 493 1204 3939 493 Size: 711 2735 2702 Fragments arranged by size: 2735 2702 711 BsaBI GATnn'nnATC Cuts at: 3000 6142 3000 Size: 3142 3006 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3955 4337 4972 275 Size: 53 83 186 936 2422 382 635 1451 Fragments arranged by size: 2422 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2211 4581 4979 5253 5400 5811 2211 Size: 2370 398 274 147 411 2548 Fragments arranged by size: 2548 2370 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2323 2353 3079 3109 1178 Size: 30 165 30 920 30 726 30 4217 Fragments arranged by size: 4217 920 726 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2110 2447 1186 Size: 924 337 4887 Fragments arranged by size: 4887 924 337 BseYI C'CCAG_C Cuts at: 2055 5351 5847 2055 Size: 3296 496 2356 Fragments arranged by size: 3296 2356 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5575 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2634 3040 3159 4209 4294 5365 2634 Size: 406 119 1050 85 1071 3417 Fragments arranged by size: 3417 1071 1050 406 119 85 BsmI GAATG_Cn' Cuts at: 2011 3099 3659 3752 2011 Size: 1088 560 93 4407 Fragments arranged by size: 4407 1088 560 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2454 3283 2454 Size: 829 5319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2634 3040 3159 3467 4209 4294 5365 Size: 399 844 406 119 308 742 85 1071 Cuts at: 5365 1391 Size: 2174 Fragments arranged by size: 2174 1071 844 742 406 399 308 119 85 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 3142 3325 4037 5767 3142 Size: 183 712 1730 3523 Fragments arranged by size: 3523 1730 712 183 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3503 844 Size: 2659 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4035 1394 Size: 429 2212 3507 Fragments arranged by size: 3507 2212 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3171 3410 4643 4817 65 Size: 1183 1923 239 1233 174 1396 Fragments arranged by size: 1923 1396 1233 1183 239 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 3096 794 Size: 2302 3846 BstBI TT'CG_AA Cuts at: 2387 3361 4952 2387 Size: 974 1591 3583 Fragments arranged by size: 3583 1591 974 BstEII G'GTnAC_C Cuts at: 1184 2438 1184 Size: 1254 4894 BstYI r'GATC_y Cuts at: 1972 2099 2408 2811 2860 3478 3845 4230 4247 Size: 127 309 403 49 618 367 385 17 Cuts at: 4247 5699 5785 5797 6143 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 618 403 385 367 346 309 127 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5596 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 2302 2571 3664 4458 4478 2302 Size: 269 1093 794 20 3972 Fragments arranged by size: 3972 1093 794 269 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 2152 3452 2152 Size: 1300 4848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2903 5155 809 Size: 507 1587 2252 1802 Fragments arranged by size: 2252 1802 1587 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3595 4484 6113 Size: 54 1425 50 275 18 1765 889 1629 Cuts at: 6113 8 Size: 43 Fragments arranged by size: 1765 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2384 4078 1353 Size: 734 297 1694 3423 Fragments arranged by size: 3423 1694 734 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3038 3038 Size: 6148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2885 4211 4808 5595 5863 1239 Size: 393 669 584 1326 597 787 268 1524 Fragments arranged by size: 1524 1326 787 669 597 584 393 268 EcoO109I rG'GnC_Cy Cuts at: 2184 3185 2184 Size: 1001 5147 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 EcoRV GAT'ATC Cuts at: 2959 2959 Size: 6148 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 5295 1678 Size: 3617 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2323 2355 2469 2501 2791 Size: 32 135 32 751 32 114 32 290 Cuts at: 2791 2823 4789 4821 4863 4895 1373 Size: 32 1966 32 42 32 2626 Fragments arranged by size: 2626 1966 751 290 135 114 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2224 2971 3492 3738 669 Size: 988 281 286 747 521 246 3079 Fragments arranged by size: 3079 988 747 521 286 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3407 5262 5446 1305 Size: 2102 1855 184 2007 Fragments arranged by size: 2102 2007 1855 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4326 Fragments arranged by size: 4326 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2703 4106 4465 4624 518 Size: 648 1537 1403 359 159 2042 Fragments arranged by size: 2042 1537 1403 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3564 4241 5389 5634 1606 Size: 1958 677 1148 245 2120 Fragments arranged by size: 2120 1958 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5596 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NsiI A_TGCA'T Cuts at: 2559 2559 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 2557 2561 3506 5051 1132 Size: 97 1328 4 945 1545 2229 Fragments arranged by size: 2229 1545 1328 945 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PfoI T'CCnGG_A Cuts at: 2411 2411 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2184 3185 2184 Size: 1001 5147 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PstI C_TGCA'G Cuts at: 830 2309 3500 830 Size: 1479 1191 3478 Fragments arranged by size: 3478 1479 1191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 SacI G_AGCT'C Cuts at: 3040 3040 Size: 6148 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SapI GCTCTTCn'nnn_ Cuts at: 2384 2384 Size: 6148 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 2644 2740 3010 4396 6108 2644 Size: 96 270 1386 1712 2684 Fragments arranged by size: 2684 1712 1386 270 96 SexAI A'CCwGG_T Cuts at: 3354 3354 Size: 6148 SfcI C'TryA_G Cuts at: 826 1045 2305 3031 3496 4631 5312 5503 826 Size: 219 1260 726 465 1135 681 191 1471 Fragments arranged by size: 1471 1260 1135 726 681 465 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4252 5153 5415 820 Size: 197 926 2309 901 262 1553 Fragments arranged by size: 2309 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 2557 3506 1132 Size: 1425 949 3774 Fragments arranged by size: 3774 1425 949 SspI AAT'ATT Cuts at: 5 52 2878 3113 3180 4072 5 Size: 47 2826 235 67 892 2081 Fragments arranged by size: 2826 2081 892 235 67 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 3118 3399 3587 513 Size: 552 2053 281 188 3074 Fragments arranged by size: 3074 2053 552 281 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2489 4175 4192 4345 4530 4975 325 Size: 1255 909 1686 17 153 185 445 1498 Fragments arranged by size: 1686 1498 1255 909 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2642 2738 3008 Size: 274 80 33 51 1443 665 96 270 Cuts at: 3008 4394 6106 96 Size: 1386 1712 138 Fragments arranged by size: 1712 1443 1386 665 274 270 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6108 6140 6108 Size: 32 6116 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 4277 4277 Size: 6148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI