(Circular) (Six-base) MAPSORT of: pFN21AE2066.seq Check: 1399 from: 1 to: 6880 pFN21AE2066 6880 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4690 278 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4223 1937 Size: 2286 4594 Acc65I G'GTAC_C Cuts at: 4201 4201 Size: 6880 AclI AA'CG_TT Cuts at: 1563 5007 5380 1563 Size: 3444 373 3063 Fragments arranged by size: 3444 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2181 2721 3258 3747 4130 4943 6327 6595 Size: 549 540 537 489 383 813 1384 268 Cuts at: 6595 1632 Size: 1917 Fragments arranged by size: 1917 1384 813 549 540 537 489 383 268 AflII C'TTAA_G Cuts at: 820 1017 2819 3425 820 Size: 197 1802 606 4275 Fragments arranged by size: 4275 1802 606 197 AflIII A'CryG_T Cuts at: 1203 5779 6756 1203 Size: 4576 977 1327 Fragments arranged by size: 4576 1327 977 AgeI A'CCGG_T Cuts at: 5711 5711 Size: 6880 AhdI GACnn_n'nnGTC Cuts at: 5609 5609 Size: 6880 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6880 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6880 AlwNI CAG_nnn'CTG Cuts at: 2329 4623 6195 2329 Size: 2294 1572 3014 Fragments arranged by size: 3014 2294 1572 ApaLI G'TGCA_C Cuts at: 2055 4937 6093 2055 Size: 2882 1156 2842 Fragments arranged by size: 2882 2842 1156 ApoI r'AATT_y Cuts at: 1493 2491 2499 2785 2808 3904 4099 4189 4415 Size: 998 8 286 23 1096 195 90 226 Cuts at: 4415 4567 6676 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1096 998 286 226 195 152 90 23 8 AseI AT'TA_AT Cuts at: 160 1032 5434 160 Size: 872 4402 1606 Fragments arranged by size: 4402 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 AvaI C'yCGr_G Cuts at: 1870 1943 4205 1870 Size: 73 2262 4545 Fragments arranged by size: 4545 2262 73 BamHI G'GATC_C Cuts at: 2319 4210 4577 2319 Size: 1891 367 4622 Fragments arranged by size: 4622 1891 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2303 4201 5656 618 Size: 325 217 627 233 283 1898 1455 1842 Fragments arranged by size: 1898 1842 1455 627 325 283 233 217 BanII G_rGCy'C Cuts at: 1391 3518 4153 4199 1391 Size: 2127 635 46 4072 Fragments arranged by size: 4072 2127 635 46 BbeI G_GCGC'C Cuts at: 2024 2024 Size: 6880 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2208 2916 3065 3660 4118 928 Size: 971 309 708 149 595 458 3690 Fragments arranged by size: 3690 971 708 595 458 309 149 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5071 5105 1903 Size: 34 3134 34 3678 Fragments arranged by size: 3678 3134 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2122 4772 5982 2122 Size: 2650 1210 3020 Fragments arranged by size: 3020 2650 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5491 136 Size: 107 122 71 631 605 3819 1525 Fragments arranged by size: 3819 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2869 3421 3547 6875 2869 Size: 552 126 3328 2874 Fragments arranged by size: 3328 2874 552 126 BlpI GC'TnA_GC Cuts at: 2565 4297 2565 Size: 1732 5148 Bme1580I G_kGCm'C Cuts at: 1790 2059 2308 4941 6097 1790 Size: 269 249 2633 1156 2573 Fragments arranged by size: 2633 2573 1156 269 249 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6880 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2767 4611 5569 Size: 416 53 83 399 24 1350 1844 958 Cuts at: 5569 442 Size: 1753 Fragments arranged by size: 1844 1753 1350 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6880 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2049 2663 3308 3452 5540 1239 Size: 810 614 645 144 2088 2579 Fragments arranged by size: 2579 2088 810 645 614 144 Bpu10I CC'TnA_GC Cuts at: 3849 3849 Size: 6880 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2607 2852 3623 5005 5870 6168 2607 Size: 245 771 1382 865 298 3319 Fragments arranged by size: 3319 1382 865 771 298 245 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5543 882 Size: 617 4044 2219 Fragments arranged by size: 4044 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4671 493 Size: 711 3467 2702 Fragments arranged by size: 3467 2702 711 BsaBI GATnn'nnATC Cuts at: 6874 6874 Size: 6880 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 3745 4687 5069 Size: 53 83 186 936 488 1724 942 382 Cuts at: 5069 5704 275 Size: 635 1451 Fragments arranged by size: 1724 1451 942 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5313 5711 5985 6132 6543 5313 Size: 398 274 147 411 5650 Fragments arranged by size: 5650 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6655 Fragments arranged by size: 6655 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2849 3740 1186 Size: 1663 891 4326 Fragments arranged by size: 4326 1663 891 BseYI C'CCAG_C Cuts at: 3467 6083 6579 3467 Size: 2616 496 3768 Fragments arranged by size: 3768 2616 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2355 3864 1163 Size: 573 619 1509 4179 Fragments arranged by size: 4179 1509 619 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4330 5091 6119 1540 Size: 275 186 2329 761 1028 2301 Fragments arranged by size: 2329 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2059 3518 4153 4941 5026 6097 2059 Size: 1459 635 788 85 1071 2842 Fragments arranged by size: 2842 1459 1071 788 635 85 BsmI GAATG_Cn' Cuts at: 4391 4484 4391 Size: 93 6787 BsmBI CGTCTCn'nnnn_ Cuts at: 3752 3752 Size: 6880 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2308 3518 4153 4199 4941 5026 Size: 399 269 249 1210 635 46 742 85 Cuts at: 5026 6097 1391 Size: 1071 2174 Fragments arranged by size: 2174 1210 1071 742 635 399 269 249 85 46 BspEI T'CCGG_A Cuts at: 6543 6543 Size: 6880 BspHI T'CATG_A Cuts at: 4084 4769 6499 4084 Size: 685 1730 4465 Fragments arranged by size: 4465 1730 685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2584 4235 844 Size: 1740 1651 3489 Fragments arranged by size: 3489 1740 1651 BsrBI CCG'CTC Cuts at: 1394 1823 4767 1394 Size: 429 2944 3507 Fragments arranged by size: 3507 2944 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5375 5549 65 Size: 1183 4127 174 1396 Fragments arranged by size: 4127 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5524 5711 1700 Size: 110 3714 187 2869 Fragments arranged by size: 3714 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6880 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6880 BssSI C'ACGA_G Cuts at: 4940 5952 4940 Size: 1012 5868 BstAPI GCAn_nnn'nTGC Cuts at: 794 2405 794 Size: 1611 5269 BstBI TT'CG_AA Cuts at: 5684 5684 Size: 6880 BstEII G'GTnAC_C Cuts at: 1184 2480 2596 1184 Size: 1296 116 5468 Fragments arranged by size: 5468 1296 116 BstYI r'GATC_y Cuts at: 1972 2319 2326 2869 3421 3547 4210 4577 4962 Size: 347 7 543 552 126 663 367 385 Cuts at: 4962 4979 6431 6517 6529 6875 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 663 552 543 385 367 347 346 126 86 17 12 7 Bsu36I CC'TnA_GG Cuts at: 2016 2016 Size: 6880 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6328 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6141 Fragments arranged by size: 6141 683 56 BtsI GCAGTG_nn' Cuts at: 4396 5190 5210 4396 Size: 794 20 6066 Fragments arranged by size: 6066 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6880 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6845 DraI TTT'AAA Cuts at: 2137 4184 2137 Size: 2047 4833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5887 809 Size: 507 4571 1802 Fragments arranged by size: 4571 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3459 4327 5216 Size: 54 1425 50 275 18 1629 868 889 Cuts at: 5216 6845 8 Size: 1629 43 Fragments arranged by size: 1629 1629 1425 889 868 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4327 1537 Size: 275 2515 4090 Fragments arranged by size: 4090 2515 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2610 3210 4810 1353 Size: 1257 600 1600 3423 Fragments arranged by size: 3423 1600 1257 600 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2332 5467 5841 5987 387 Size: 1064 138 743 3135 374 146 1280 Fragments arranged by size: 3135 1280 1064 743 374 146 138 EcoICRI GAG'CTC Cuts at: 3516 4151 3516 Size: 635 6245 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2181 2663 2721 3258 3308 3452 Size: 393 417 132 482 58 537 50 144 Cuts at: 3452 3747 4130 4943 5540 6327 6595 1239 Size: 295 383 813 597 787 268 1524 Fragments arranged by size: 1524 813 787 597 537 482 417 393 383 295 268 144 132 58 50 EcoRI G'AATT_C Cuts at: 2499 4189 4567 2499 Size: 1690 378 4812 Fragments arranged by size: 4812 1690 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2041 2041 Size: 6880 FspI TGC'GCA Cuts at: 5386 5386 Size: 6880 HaeII r_GCGC'y Cuts at: 1678 2024 6027 1678 Size: 346 4003 2531 Fragments arranged by size: 4003 2531 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2311 2343 2672 2704 3061 Size: 32 135 32 739 32 329 32 357 Cuts at: 3061 3093 3406 3425 3438 3457 3505 3537 5521 Size: 32 313 19 13 19 48 32 1984 Cuts at: 5521 5553 5595 5627 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1984 739 357 329 313 135 48 42 32 32 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2173 2920 3402 4224 4470 669 Size: 988 281 235 747 482 822 246 3079 Fragments arranged by size: 3079 988 822 747 482 281 246 235 HindIII A'AGCT_T Cuts at: 748 2506 3008 748 Size: 1758 502 4620 Fragments arranged by size: 4620 1758 502 HpaI GTT'AAC Cuts at: 3402 4470 3402 Size: 1068 5812 KasI G'GCGC_C Cuts at: 2020 2020 Size: 6880 KpnI G_GTAC'C Cuts at: 4205 4205 Size: 6880 MfeI C'AATT_G Cuts at: 3209 4479 3209 Size: 1270 5610 MluI A'CGCG_T Cuts at: 6756 6756 Size: 6880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2322 5994 6178 1305 Size: 1017 3672 184 2007 Fragments arranged by size: 3672 2007 1017 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5058 Fragments arranged by size: 5058 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 4838 5197 5356 518 Size: 648 1416 2256 359 159 2042 Fragments arranged by size: 2256 2042 1416 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2890 3670 3984 4296 4973 6121 6366 1606 Size: 1284 780 314 312 677 1148 245 2120 Fragments arranged by size: 2120 1284 1148 780 677 314 312 245 NarI GG'CG_CC Cuts at: 2021 2021 Size: 6880 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6328 NdeI CA'TA_TG Cuts at: 387 3880 387 Size: 3493 3387 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6880 NotI GC'GGCC_GC Cuts at: 4327 4327 Size: 6880 NspI r_CATG'y Cuts at: 1132 1229 3929 4238 5783 1132 Size: 97 2700 309 1545 2229 Fragments arranged by size: 2700 2229 1545 309 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6880 PciI A'CATG_T Cuts at: 5779 5779 Size: 6880 PflMI CCAn_nnn'nTGG Cuts at: 1108 2329 1108 Size: 1221 5659 PfoI T'CCnGG_A Cuts at: 2322 2322 Size: 6880 PmeI GTTT'AAAC Cuts at: 4184 4184 Size: 6880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4949 4981 6462 6494 4949 Size: 32 1481 32 5335 Fragments arranged by size: 5335 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6880 PsiI TTA'TAA Cuts at: 4450 4450 Size: 6880 PstI C_TGCA'G Cuts at: 830 2595 3555 4232 830 Size: 1765 960 677 3478 Fragments arranged by size: 3478 1765 960 677 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6880 PvuII CAG'CTG Cuts at: 2890 3670 2890 Size: 780 6100 SacI G_AGCT'C Cuts at: 3518 4153 3518 Size: 635 6245 SalI G'TCGA_C Cuts at: 1936 4222 1936 Size: 2286 4594 SbfI CC_TGCA'GG Cuts at: 4232 4232 Size: 6880 ScaI AGT'ACT Cuts at: 5128 6840 5128 Size: 1712 5168 SfcI C'TryA_G Cuts at: 826 1045 2291 2591 3551 4228 5363 6044 6235 Size: 219 1246 300 960 677 1135 681 191 Cuts at: 6235 826 Size: 1471 Fragments arranged by size: 1471 1246 1135 960 681 677 300 219 191 SfoI GGC'GCC Cuts at: 2022 2022 Size: 6880 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6880 SmaI CCC'GGG Cuts at: 1872 4207 1872 Size: 2335 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2622 2819 2831 3425 3638 4984 Size: 197 926 679 197 12 594 213 1346 Cuts at: 4984 5885 6147 820 Size: 901 262 1553 Fragments arranged by size: 1553 1346 926 901 679 594 262 213 197 197 12 SnaBI TAC'GTA Cuts at: 493 4671 493 Size: 4178 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6880 SphI G_CATG'C Cuts at: 1132 4238 1132 Size: 3106 3774 SspI AAT'ATT Cuts at: 5 52 2842 4804 5 Size: 47 2790 1962 2081 Fragments arranged by size: 2790 2081 1962 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6880 StyI C'CwwG_G Cuts at: 513 1065 3462 4319 513 Size: 552 2397 857 3074 Fragments arranged by size: 3074 2397 857 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 4907 4924 5077 5262 5707 325 Size: 1255 1035 2292 17 153 185 445 1498 Fragments arranged by size: 2292 1498 1255 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5126 6838 96 Size: 274 80 33 51 1443 3149 1712 138 Fragments arranged by size: 3149 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6840 6872 6840 Size: 32 6848 Tth111I GACn'n_nGTC Cuts at: 1316 2915 3559 1316 Size: 1599 644 4637 Fragments arranged by size: 4637 1599 644 XbaI T'CTAG_A Cuts at: 3230 3544 4216 3230 Size: 314 672 5894 Fragments arranged by size: 5894 672 314 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6880 XmaI C'CCGG_G Cuts at: 1870 4205 1870 Size: 2335 4545 XmnI GAAnn'nnTTC Cuts at: 2033 3009 5009 2033 Size: 976 2000 3904 Fragments arranged by size: 3904 2000 976 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4688 276 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI