(Linear) (Six-base) MAPSORT of: KIEE2066.seq Check: 6771 from: 1 to: 2175 KIEE2066.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 176 716 1253 1742 2125 2175 Size: 176 540 537 489 383 50 Fragments arranged by size: 540 537 489 383 176 50 AflII C'TTAA_G Cuts at: 0 814 1420 2175 Size: 814 606 755 Fragments arranged by size: 814 755 606 AlwNI CAG_nnn'CTG Cuts at: 0 324 2175 Size: 324 1851 ApaLI G'TGCA_C Cuts at: 0 50 2175 Size: 50 2125 ApoI r'AATT_y Cuts at: 0 486 494 780 803 1899 2094 2175 Size: 486 8 286 23 1096 195 81 Fragments arranged by size: 1096 486 286 195 81 23 8 BamHI G'GATC_C Cuts at: 0 314 2175 Size: 314 1861 BanI G'GyrC_C Cuts at: 0 15 298 2175 Size: 15 283 1877 Fragments arranged by size: 1877 283 15 BanII G_rGCy'C Cuts at: 0 1513 2148 2175 Size: 1513 635 27 Fragments arranged by size: 1513 635 27 BbeI G_GCGC'C Cuts at: 0 19 2175 Size: 19 2156 BbsI GAAGACnn'nnnn_ Cuts at: 0 203 911 1060 1655 2113 2175 Size: 203 708 149 595 458 62 Fragments arranged by size: 708 595 458 203 149 62 BciVI GTATCCnnnnn_n' Cuts at: 0 117 2175 Size: 117 2058 BglII A'GATC_T Cuts at: 0 864 1416 1542 2175 Size: 864 552 126 633 Fragments arranged by size: 864 633 552 126 BlpI GC'TnA_GC Cuts at: 0 560 2175 Size: 560 1615 Bme1580I G_kGCm'C Cuts at: 0 54 303 2175 Size: 54 249 1872 Fragments arranged by size: 1872 249 54 BmrI ACTGGGnnnn_n' Cuts at: 0 762 2175 Size: 762 1413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 658 1303 1447 2175 Size: 44 614 645 144 728 Fragments arranged by size: 728 645 614 144 44 Bpu10I CC'TnA_GC Cuts at: 0 1844 2175 Size: 1844 331 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 602 847 1618 2175 Size: 602 245 771 557 Fragments arranged by size: 771 602 557 245 BsaHI Gr'CG_yC Cuts at: 0 16 1740 2175 Size: 16 1724 435 Fragments arranged by size: 1724 435 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 844 1735 2175 Size: 844 891 440 Fragments arranged by size: 891 844 440 BseYI C'CCAG_C Cuts at: 0 1462 2175 Size: 1462 713 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 350 1859 2175 Size: 350 1509 316 Fragments arranged by size: 1509 350 316 BsiHKAI G_wGCw'C Cuts at: 0 54 1513 2148 2175 Size: 54 1459 635 27 Fragments arranged by size: 1459 635 54 27 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1747 2175 Size: 1747 428 Bsp1286I G_dGCh'C Cuts at: 0 54 303 1513 2148 2175 Size: 54 249 1210 635 27 Fragments arranged by size: 1210 635 249 54 27 BspHI T'CATG_A Cuts at: 0 2079 2175 Size: 2079 96 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 579 2175 Size: 579 1596 BstAPI GCAn_nnn'nTGC Cuts at: 0 400 2175 Size: 400 1775 BstEII G'GTnAC_C Cuts at: 0 475 591 2175 Size: 475 116 1584 Fragments arranged by size: 1584 475 116 BstYI r'GATC_y Cuts at: 0 314 321 864 1416 1542 2175 Size: 314 7 543 552 126 633 Fragments arranged by size: 633 552 543 314 126 7 Bsu36I CC'TnA_GG Cuts at: 0 11 2175 Size: 11 2164 DraI TTT'AAA Cuts at: 0 132 2175 Size: 132 2043 EaeI y'GGCC_r Cuts at: 0 1454 2175 Size: 1454 721 EarI CTCTTCn'nnn_ Cuts at: 0 605 1205 2175 Size: 605 600 970 Fragments arranged by size: 970 605 600 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 327 2175 Size: 327 1848 EcoICRI GAG'CTC Cuts at: 0 1511 2146 2175 Size: 1511 635 29 Fragments arranged by size: 1511 635 29 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 176 658 716 1253 1303 1447 1742 Size: 44 132 482 58 537 50 144 295 Cuts at: 1742 2125 2175 Size: 383 50 Fragments arranged by size: 537 482 383 295 144 132 58 50 50 44 EcoRI G'AATT_C Cuts at: 0 494 2175 Size: 494 1681 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 36 2175 Size: 36 2139 HaeII r_GCGC'y Cuts at: 0 19 2175 Size: 19 2156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 306 338 667 699 1056 1088 1401 1420 Size: 306 32 329 32 357 32 313 19 Cuts at: 1420 1433 1452 1500 1532 2175 Size: 13 19 48 32 643 Fragments arranged by size: 643 357 329 313 306 48 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 0 168 915 1397 2175 Size: 168 747 482 778 Fragments arranged by size: 778 747 482 168 HindIII A'AGCT_T Cuts at: 0 501 1003 2175 Size: 501 502 1172 Fragments arranged by size: 1172 502 501 HpaI GTT'AAC Cuts at: 0 1397 2175 Size: 1397 778 KasI G'GCGC_C Cuts at: 0 15 2175 Size: 15 2160 MfeI C'AATT_G Cuts at: 0 1204 2175 Size: 1204 971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 317 2175 Size: 317 1858 MslI CAynn'nnrTG Cuts at: 0 577 2175 Size: 577 1598 MspA1I CmG'CkG Cuts at: 0 885 1665 1979 2175 Size: 885 780 314 196 Fragments arranged by size: 885 780 314 196 NarI GG'CG_CC Cuts at: 0 16 2175 Size: 16 2159 NdeI CA'TA_TG Cuts at: 0 1875 2175 Size: 1875 300 NspI r_CATG'y Cuts at: 0 1924 2175 Size: 1924 251 PflMI CCAn_nnn'nTGG Cuts at: 0 324 2175 Size: 324 1851 PfoI T'CCnGG_A Cuts at: 0 317 2175 Size: 317 1858 PstI C_TGCA'G Cuts at: 0 590 1550 2175 Size: 590 960 625 Fragments arranged by size: 960 625 590 PvuII CAG'CTG Cuts at: 0 885 1665 2175 Size: 885 780 510 Fragments arranged by size: 885 780 510 SacI G_AGCT'C Cuts at: 0 1513 2148 2175 Size: 1513 635 27 Fragments arranged by size: 1513 635 27 SfcI C'TryA_G Cuts at: 0 286 586 1546 2175 Size: 286 300 960 629 Fragments arranged by size: 960 629 300 286 SfoI GGC'GCC Cuts at: 0 17 2175 Size: 17 2158 SmlI C'TyrA_G Cuts at: 0 617 814 826 1420 1633 2175 Size: 617 197 12 594 213 542 Fragments arranged by size: 617 594 542 213 197 12 SspI AAT'ATT Cuts at: 0 837 2175 Size: 837 1338 StyI C'CwwG_G Cuts at: 0 1457 2175 Size: 1457 718 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 610 2175 Size: 610 1565 Tth111I GACn'n_nGTC Cuts at: 0 910 1554 2175 Size: 910 644 621 Fragments arranged by size: 910 644 621 XbaI T'CTAG_A Cuts at: 0 1225 1539 2175 Size: 1225 314 636 Fragments arranged by size: 1225 636 314 XmnI GAAnn'nnTTC Cuts at: 0 28 1004 2175 Size: 28 976 1171 Fragments arranged by size: 1171 976 28 Enzymes that do cut: AcuI AflII AlwNI ApaLI ApoI BamHI BanI BanII BbeI BbsI BciVI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BstAPI BstEII BstYI Bsu36I DraI EaeI EarI EciI EcoICRI Eco57MI EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MslI MspA1I NarI NdeI NspI PflMI PfoI PstI PvuII SacI SfcI SfoI SmlI SspI StyI TaqII Tth111I XbaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI BclI BfrBI BglI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI EcoNI EcoO109I EcoRV FseI FspI FspAI KpnI MluI MscI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI XcmI XhoI XmaI ZraI