################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MboI MfeI MflI MluI MunI MvnI NaeI NarI NciI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 114 TCCGGA T^CCGGA ApaI 926 GGGCCC GGGCC^C BanII 926 GGGCCC G[GA]GC[CT]^C BbsI 865 GTCTTC GAAGAC(2/6) BciVI 111 GTATCC GTATCC(6/5) BpuAI 865 GTCTTC GAAGAC(2/6) BsaBI 106 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 114 TCCGGA [AT]^CCGG[AT] BseAI 114 TCCGGA T^CCGGA BseRI 607 GAGGAG GAGGAG(10/8) BspEI 114 TCCGGA T^CCGGA BsrI 258 CCAGT ACTGG(1/-1) BssSI 58 CTCGTG CACGAG(-5/-1) DraI 348 TTTAAA TTT^AAA DsaI 887 CCACGG C^C[GA][CT]GG EarI 483 CTCTTC CTCTTC(1/4) EcoRI 741 GAATTC G^AATTC FauI 924 GCGGG CCCGC(4/6) HapII 115 CCGG C^CGG HpaII 115 CCGG C^CGG Ksp632I 483 CTCTTC CTCTTC(1/4) MamI 106 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 114 TCCGGA T^CCGGA MslI 915 CATTAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 115 CCGG C^CGG NdeI 66 CATATG CA^TATG NlaIII 630 CATG CATG^ NlaIV 926 GGGCCC GG[ACGT]^[ACGT]CC PflMI 9 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 482 GCTCTTC GCTCTTC(1/4) SmlI 652 CTCAAG C^T[CT][GA]AG Van91I 9 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 620 CCATTGAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 329 GAATTT [GA]^AATT[CT] 741 GAATTC AlwNI 588 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 599 CAGCTGCTG ApoI 329 GAATTT [GA]^AATT[CT] 741 GAATTC BalI 62 TGGCCA TGG^CCA 255 TGGCCA BbvCI 881 CCTCAGC CCTCAGC(-5/-2) 604 GCTGAGG BmyI 926 GGGCCC G[AGT]GC[ACT]^C 973 GTGCCC BpmI 125 CTGGAG CTGGAG(16/14) 783 CTGGAG BsmFI 545 GGGAC GGGAC(10/14) 689 GGGAC Bsp1286I 926 GGGCCC G[AGT]GC[ACT]^C 973 GTGCCC BspMI 421 ACCTGC ACCTGC(4/8) 750 GCAGGT EaeI 62 TGGCCA [CT]^GGCC[GA] 255 TGGCCA EcoT14I 182 CCTTGG C^C[AT][AT]GG 495 CCAAGG MaeII 414 ACGT A^CGT 505 ACGT MluNI 62 TGGCCA TGG^CCA 255 TGGCCA MscI 62 TGGCCA TGG^CCA 255 TGGCCA MseI 349 TTAA T^TAA 736 TTAA PstI 527 CTGCAG CTGCA^G 978 CTGCAG StyI 182 CCTTGG C^C[AT][AT]GG 495 CCAAGG Tru9I 349 TTAA T^TAA 736 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MspA1I MvaI MwoI PleI PvuII RsaI Sau96I ScaI ScrFI SfaNI SfcI TseI Tsp45I Tsp509I TspRI