(Linear) (Six-base) MAPSORT of: KIEE2528.seq Check: 7518 from: 1 to: 1347 KIEE2528.orf With 182 enzymes: SgfI * June 11, 2014 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 715 840 1347 Size: 715 125 507 Fragments arranged by size: 715 507 125 AatII G_ACGT'C Cuts at: 0 1337 1347 Size: 1337 10 AccI GT'mk_AC Cuts at: 0 1119 1347 Size: 1119 228 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 98 1347 Size: 36 62 1249 Fragments arranged by size: 1249 62 36 AflIII A'CryG_T Cuts at: 0 535 1292 1347 Size: 535 757 55 Fragments arranged by size: 757 535 55 AleI CACnn'nnGTG Cuts at: 0 859 1347 Size: 859 488 ApaI G_GGCC'C Cuts at: 0 402 1111 1347 Size: 402 709 236 Fragments arranged by size: 709 402 236 ApoI r'AATT_y Cuts at: 0 513 700 1347 Size: 513 187 647 Fragments arranged by size: 647 513 187 AvaI C'yCGr_G Cuts at: 0 893 1347 Size: 893 454 AvrII C'CTAG_G Cuts at: 0 846 1347 Size: 846 501 BanI G'GyrC_C Cuts at: 0 1150 1347 Size: 1150 197 BanII G_rGCy'C Cuts at: 0 402 886 1111 1347 Size: 402 484 225 236 Fragments arranged by size: 484 402 236 225 BbeI G_GCGC'C Cuts at: 0 1154 1347 Size: 1154 193 Bme1580I G_kGCm'C Cuts at: 0 402 1111 1347 Size: 402 709 236 Fragments arranged by size: 709 402 236 BmrI ACTGGGnnnn_n' Cuts at: 0 184 633 1347 Size: 184 449 714 Fragments arranged by size: 714 449 184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 751 1091 1347 Size: 751 340 256 Fragments arranged by size: 751 340 256 Bpu10I CC'TnA_GC Cuts at: 0 436 1347 Size: 436 911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 687 1347 Size: 687 660 BsaI GGTCTCn'nnnn_ Cuts at: 0 31 1347 Size: 31 1316 BsaHI Gr'CG_yC Cuts at: 0 1151 1334 1347 Size: 1151 183 13 Fragments arranged by size: 1151 183 13 BsaWI w'CCGG_w Cuts at: 0 148 1347 Size: 148 1199 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1219 1347 Size: 1219 128 BseYI C'CCAG_C Cuts at: 0 772 1110 1347 Size: 772 338 237 Fragments arranged by size: 772 338 237 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 660 812 1169 1347 Size: 631 29 152 357 178 Fragments arranged by size: 631 357 178 152 29 BsiHKAI G_wGCw'C Cuts at: 0 866 886 1347 Size: 866 20 461 Fragments arranged by size: 866 461 20 Bsp1286I G_dGCh'C Cuts at: 0 402 866 886 1111 1347 Size: 402 464 20 225 236 Fragments arranged by size: 464 402 236 225 20 BspEI T'CCGG_A Cuts at: 0 148 1347 Size: 148 1199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 603 715 840 1347 Size: 603 112 125 507 Fragments arranged by size: 603 507 125 112 BsrBI CCG'CTC Cuts at: 0 735 1347 Size: 735 612 BsrDI GCAATG_nn' Cuts at: 0 233 289 1347 Size: 233 56 1058 Fragments arranged by size: 1058 233 56 BsrGI T'GTAC_A Cuts at: 0 2 1347 Size: 2 1345 BssSI C'ACGA_G Cuts at: 0 836 1347 Size: 836 511 BstYI r'GATC_y Cuts at: 0 440 800 1347 Size: 440 360 547 Fragments arranged by size: 547 440 360 BtgI C'CryG_G Cuts at: 0 248 500 521 1271 1347 Size: 248 252 21 750 76 Fragments arranged by size: 750 252 248 76 21 DraI TTT'AAA Cuts at: 0 180 1347 Size: 180 1167 DrdI GACnn_nn'nnGTC Cuts at: 0 259 1347 Size: 259 1088 EarI CTCTTCn'nnn_ Cuts at: 0 23 650 1225 1347 Size: 23 627 575 122 Fragments arranged by size: 627 575 122 23 EcoICRI GAG'CTC Cuts at: 0 884 1347 Size: 884 463 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 98 751 1091 1347 Size: 36 62 653 340 256 Fragments arranged by size: 653 340 256 62 36 EcoO109I rG'GnC_Cy Cuts at: 0 184 398 1347 Size: 184 214 949 Fragments arranged by size: 949 214 184 HaeII r_GCGC'y Cuts at: 0 1154 1347 Size: 1154 193 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1123 1155 1237 1269 1319 1347 Size: 1123 32 82 32 50 28 Fragments arranged by size: 1123 82 50 32 32 28 HincII GTy'rAC Cuts at: 0 264 996 1347 Size: 264 732 351 Fragments arranged by size: 732 351 264 HindIII A'AGCT_T Cuts at: 0 555 1347 Size: 555 792 HpaI GTT'AAC Cuts at: 0 996 1347 Size: 996 351 KasI G'GCGC_C Cuts at: 0 1150 1347 Size: 1150 197 MslI CAynn'nnrTG Cuts at: 0 407 859 940 1347 Size: 407 452 81 407 Fragments arranged by size: 452 407 407 81 NarI GG'CG_CC Cuts at: 0 1151 1347 Size: 1151 196 NcoI C'CATG_G Cuts at: 0 248 500 521 1271 1347 Size: 248 252 21 750 76 Fragments arranged by size: 750 252 248 76 21 NspI r_CATG'y Cuts at: 0 539 1296 1347 Size: 539 757 51 Fragments arranged by size: 757 539 51 PciI A'CATG_T Cuts at: 0 535 1292 1347 Size: 535 757 55 Fragments arranged by size: 757 535 55 PfoI T'CCnGG_A Cuts at: 0 122 445 1347 Size: 122 323 902 Fragments arranged by size: 902 323 122 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 706 738 1347 Size: 706 32 609 Fragments arranged by size: 706 609 32 PpuMI rG'GwC_Cy Cuts at: 0 184 1347 Size: 184 1163 PspOMI G'GGCC_C Cuts at: 0 398 1107 1347 Size: 398 709 240 Fragments arranged by size: 709 398 240 SacI G_AGCT'C Cuts at: 0 886 1347 Size: 886 461 SfcI C'TryA_G Cuts at: 0 255 465 1120 1347 Size: 255 210 655 227 Fragments arranged by size: 655 255 227 210 SfoI GGC'GCC Cuts at: 0 1152 1347 Size: 1152 195 SmlI C'TyrA_G Cuts at: 0 666 893 1347 Size: 666 227 454 Fragments arranged by size: 666 454 227 StyI C'CwwG_G Cuts at: 0 248 500 521 846 908 1127 1271 1347 Size: 248 252 21 325 62 219 144 76 Fragments arranged by size: 325 252 248 219 144 76 62 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 111 892 1347 Size: 111 781 455 Fragments arranged by size: 781 455 111 TatI w'GTAC_w Cuts at: 0 2 1347 Size: 2 1345 XhoI C'TCGA_G Cuts at: 0 893 1347 Size: 893 454 XmnI GAAnn'nnTTC Cuts at: 0 514 1347 Size: 514 833 ZraI GAC'GTC Cuts at: 0 1335 1347 Size: 1335 12 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI ApaI ApoI AvaI AvrII BanI BanII BbeI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssSI BstYI BtgI DraI DrdI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HindIII HpaI KasI MslI NarI NcoI NspI PciI PfoI PpiI PpuMI PspOMI SacI SfcI SfoI SmlI StyI TaqII TatI XhoI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII EaeI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI KpnI MfeI MluI MmeI MscI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XmaI