################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccII 496 CGCG CG^CG AhdI 142 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 106 GGGCCC GGGCC^C Asp700I 381 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 142 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 216 GCTGAGG CCTCAGC(-5/-2) BciVI 332 GTATCC GTATCC(6/5) BsaI 508 GAGACC GGTCTC(1/5) BsgI 525 CTGCAC GTGCAG(16/14) BsrFI 423 GCCGGT [GA]^CCGG[CT] BssHII 495 GCGCGC G^CGCGC BstUI 496 CGCG CG^CG Cfr10I 423 GCCGGT [GA]^CCGG[CT] DraIII 127 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 338 CCGTGG C^C[GA][CT]GG Eam1105I 142 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 345 CCTGCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 256 GTCAAC GT[CT]^[GA]AC HindII 256 GTCAAC GT[CT]^[GA]AC MaeIII 150 GTCAC ^GT[ACGT]AC MslI 513 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 496 CGCG CG^CG SfcI 306 CTACAG C^T[GA][CT]AG TaqI 197 TCGA T^CGA Tsp45I 150 GTCAC ^GT[GC]AC TspRI 137 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 197 TCGA T^CGA XmnI 381 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BanI 132 GGTGCC G^G[CT][GA]CC 359 GGTGCC BanII 69 GAGCCC G[GA]GC[CT]^C 106 GGGCCC BmyI 69 GAGCCC G[AGT]GC[ACT]^C 106 GGGCCC BsmAI 508 GAGAC GTCTC(1/5) 294 GTCTC Bsp1286I 69 GAGCCC G[AGT]GC[ACT]^C 106 GGGCCC BsrI 5 ACTGG ACTGG(1/-1) 138 ACTGG BstF5I 155 GGATG GGATG(2/0) 272 GGATG BstXI 41 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 512 CCATCGCTGTGG DdeI 217 CTGAG C^T[ACGT]AG 228 CTCAG DraII 106 GGGCCCC [GA]G^G[ACGT]CC[CT] 480 AGGGCCT EcoO109I 106 GGGCCCC [GA]G^G[ACGT]CC[CT] 480 AGGGCCT FokI 155 GGATG GGATG(9/13) 272 GGATG HapII 349 CCGG C^CGG 424 CCGG HpaII 349 CCGG C^CGG 424 CCGG MspA1I 214 CAGCTG C[AC]G^C[GT]G 265 CAGCTG MspI 349 CCGG C^CGG 424 CCGG PvuII 214 CAGCTG CAG^CTG 265 CAGCTG SexAI 128 ACCAGGT A^CC[AT]GGT 143 ACCTGGT SfaNI 311 GCATC GCATC(5/9) 533 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BseRI BsiYI BslI BspMI BssKI BstNI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeIII HhaI HinP1I HphI ItaI MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI