################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbvCI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoT14I EcoT22I FauI FseI HindIII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 67 GACGTC GACGT^C AccI 652 GTCTAC GT^[AC][GT]AC AcyI 67 GACGTC G[GA]^CG[CT]C AlwNI 424 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 802 GAGCAC G[AT]GC[AT]^C AvaII 60 GGACC G^G[AT]CC AviII 1025 TGCGCA TGC^GCA BalI 134 TGGCCA TGG^CCA BanII 984 GAGCCC G[GA]GC[CT]^C BbrPI 1099 CACGTG CAC^GTG BcgI 104 GCATGGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 778 AGATCT A^GATCT BsaAI 1099 CACGTG [CT]AC^GT[GA] BsaHI 67 GACGTC G[GA]^CG[CT]C BsiHKAI 802 GAGCAC G[AT]GC[AT]^C BsmFI 83 GGGAC GGGAC(10/14) BstEII 198 GGTCACC G^GT[ACGT]ACC BstPI 198 GGTCACC G^GT[ACGT]ACC BstYI 778 AGATCT [GA]^GATC[CT] DrdI 61 GACCACGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 606 CTCTTC CTCTTC(1/4) EcoNI 732 CCTTAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 198 GGTCACC G^GT[ACGT]ACC EcoRV 1094 GATATC GAT^ATC FspI 1025 TGCGCA TGC^GCA HaeII 22 AGCGCC [GA]GCGC^[CT] Hin1I 67 GACGTC G[GA]^CG[CT]C HincII 874 GTTAAC GT[CT]^[GA]AC HindII 874 GTTAAC GT[CT]^[GA]AC HpaI 874 GTTAAC GTT^AAC Ksp632I 606 CTCTTC CTCTTC(1/4) MflI 778 AGATCT [GA]^GATC[CT] MluNI 134 TGGCCA TGG^CCA MscI 134 TGGCCA TGG^CCA MseI 875 TTAA T^TAA NspI 885 GCATGT [GA]CATG^[CT] PmaCI 1099 CACGTG CAC^GTG PmlI 1099 CACGTG CAC^GTG PstI 190 CTGCAG CTGCA^G SfcI 190 CTGCAG C^T[GA][CT]AG SmlI 814 CTCAAG C^T[CT][GA]AG TaqI 182 TCGA T^CGA TfiI 1198 GATTC G^A[AT]TC Tru9I 875 TTAA T^TAA TthHB8I 182 TCGA T^CGA XhoII 778 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 686 AGCT AG^CT 1013 AGCT AlwI 891 GATCC GGATC(4/5) 387 GGATC AvaI 10 CCCGGG C^[CT]CG[GA]G 449 CCCGGG BanI 36 GGCACC G^G[CT][GA]CC 258 GGTGCC BclI 455 TGATCA T^GATCA 638 TGATCA BfaI 250 CTAG C^TAG 1184 CTAG BsaI 364 GAGACC GGTCTC(1/5) 562 GAGACC BsoBI 10 CCCGGG C^[CT]CG[GA]G 449 CCCGGG BspMI 565 ACCTGC ACCTGC(4/8) 1028 GCAGGT BsrI 222 ACTGG ACTGG(1/-1) 515 ACTGG DdeI 707 CTGAG C^T[ACGT]AG 733 CTTAG EaeI 134 TGGCCA [CT]^GGCC[GA] 353 CGGCCA FbaI 455 TGATCA T^GATCA 638 TGATCA HgaI 186 GCGTC GACGC(5/10) 347 GCGTC MaeI 250 CTAG C^TAG 1184 CTAG PleI 770 GACTC GAGTC(4/5) 1228 GACTC SmaI 10 CCCGGG CCC^GGG 449 CCCGGG StuI 483 AGGCCT AGG^CCT 1043 AGGCCT XmaCI 10 CCCGGG C^CCGGG 449 CCCGGG XmaI 10 CCCGGG C^CCGGG 449 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrDI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI