(Circular) (Six-base) MAPSORT of: pFN21AE9552.seq Check: 5398 from: 1 to: 5869 pFN21AE9552 5869 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2597 2869 2597 Size: 272 5597 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2307 3679 278 Size: 53 83 186 936 771 1372 2468 Fragments arranged by size: 2468 1372 936 771 186 83 53 AccI GT'mk_AC Cuts at: 1937 3212 1937 Size: 1275 4594 Acc65I G'GTAC_C Cuts at: 3190 3190 Size: 5869 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3932 5316 5584 1632 Size: 2300 1384 268 1917 Fragments arranged by size: 2300 1917 1384 268 AfeI AGC'GCT Cuts at: 3002 3002 Size: 5869 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 3070 4768 5745 1203 Size: 1867 1698 977 1327 Fragments arranged by size: 1867 1698 1327 977 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5869 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5869 AlwNI CAG_nnn'CTG Cuts at: 3612 5184 3612 Size: 1572 4297 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 3178 3404 3556 5665 1493 Size: 1685 226 152 2109 1697 Fragments arranged by size: 2109 1697 1685 226 152 AseI AT'TA_AT Cuts at: 160 1032 2423 3057 4423 160 Size: 872 1391 634 1366 1606 Fragments arranged by size: 1606 1391 1366 872 634 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 3194 1870 Size: 73 1251 4545 Fragments arranged by size: 4545 1251 73 BamHI G'GATC_C Cuts at: 2848 3062 3199 3566 2848 Size: 214 137 367 5151 Fragments arranged by size: 5151 367 214 137 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 2821 3190 4645 618 Size: 325 217 627 447 587 369 1455 1842 Fragments arranged by size: 1842 1455 627 587 447 369 325 217 BanII G_rGCy'C Cuts at: 1391 3188 1391 Size: 1797 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2551 3016 928 Size: 971 652 465 3781 Fragments arranged by size: 3781 971 652 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4060 4094 1903 Size: 34 2123 34 3678 Fragments arranged by size: 3678 2123 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 4971 3761 Size: 1210 4659 BclI T'GATC_A Cuts at: 1548 1548 Size: 5869 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4480 136 Size: 107 122 71 631 605 2808 1525 Fragments arranged by size: 2808 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5864 5864 Size: 5869 BlpI GC'TnA_GC Cuts at: 3153 3286 3153 Size: 133 5736 Bme1580I G_kGCm'C Cuts at: 1790 2824 3930 5086 1790 Size: 1034 1106 1156 2573 Fragments arranged by size: 2573 1156 1106 1034 BmgBI CAC'GTC Cuts at: 1318 2966 1318 Size: 1648 4221 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3153 3600 4558 Size: 416 53 83 399 24 1736 447 958 Cuts at: 4558 442 Size: 1753 Fragments arranged by size: 1753 1736 958 447 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2739 2739 Size: 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4529 1239 Size: 3290 2579 Bpu10I CC'TnA_GC Cuts at: 2153 2661 2153 Size: 508 5361 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3994 4859 5157 3994 Size: 865 298 4706 Fragments arranged by size: 4706 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2759 4532 882 Size: 617 1260 1773 2219 Fragments arranged by size: 2219 1773 1260 617 BsaAI yAC'GTr Cuts at: 493 1204 2550 3660 493 Size: 711 1346 1110 2702 Fragments arranged by size: 2702 1346 1110 711 BsaBI GATnn'nnATC Cuts at: 2102 5863 2102 Size: 3761 2108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 3676 4058 4693 Size: 53 83 186 936 771 1372 382 635 Cuts at: 4693 275 Size: 1451 Fragments arranged by size: 1451 1372 936 771 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4302 4700 4974 5121 5532 4302 Size: 398 274 147 411 4639 Fragments arranged by size: 4639 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3101 3131 1178 Size: 30 165 30 1698 30 3916 Fragments arranged by size: 3916 1698 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2612 2698 1186 Size: 865 561 86 4357 Fragments arranged by size: 4357 865 561 86 BseYI C'CCAG_C Cuts at: 2139 2428 2439 2817 5072 5568 2139 Size: 289 11 378 2255 496 2440 Fragments arranged by size: 2440 2255 496 378 289 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5296 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3319 4080 5108 1540 Size: 275 186 1318 761 1028 2301 Fragments arranged by size: 2301 1318 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3930 4015 5086 3930 Size: 85 1071 4713 Fragments arranged by size: 4713 1071 85 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2824 3188 3930 4015 5086 1391 Size: 399 1034 364 742 85 1071 2174 Fragments arranged by size: 2174 1071 1034 742 399 364 85 BspEI T'CCGG_A Cuts at: 5532 5532 Size: 5869 BspHI T'CATG_A Cuts at: 3758 5488 3758 Size: 1730 4139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2597 2869 3224 844 Size: 1280 473 272 355 3489 Fragments arranged by size: 3489 1280 473 355 272 BsrBI CCG'CTC Cuts at: 1394 1823 3756 1394 Size: 429 1933 3507 Fragments arranged by size: 3507 1933 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4364 4538 65 Size: 1183 3116 174 1396 Fragments arranged by size: 3116 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4513 4700 1700 Size: 110 2703 187 2869 Fragments arranged by size: 2869 2703 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5869 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5869 BssSI C'ACGA_G Cuts at: 3929 4941 3929 Size: 1012 4857 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5869 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2845 2245 Size: 600 5269 BstYI r'GATC_y Cuts at: 1972 2200 2679 2848 3062 3199 3566 3951 3968 Size: 228 479 169 214 137 367 385 17 Cuts at: 3968 5420 5506 5518 5864 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 479 385 367 346 228 214 169 137 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4378 Fragments arranged by size: 4378 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2401 2735 487 Size: 683 56 1175 334 3621 Fragments arranged by size: 3621 1175 683 334 56 BtsI GCAGTG_nn' Cuts at: 3093 3385 4179 4199 3093 Size: 292 794 20 4763 Fragments arranged by size: 4763 794 292 20 ClaI AT'CG_AT Cuts at: 1554 2324 1554 Size: 770 5099 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2867 4876 809 Size: 507 1551 2009 1802 Fragments arranged by size: 2009 1802 1551 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 3100 3316 Size: 54 1425 50 275 18 270 1000 216 Cuts at: 3316 4205 5834 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1000 889 275 270 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3316 1537 Size: 275 1504 4090 Fragments arranged by size: 4090 1504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3799 1353 Size: 2446 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4456 4830 4976 387 Size: 1064 138 2867 374 146 1280 Fragments arranged by size: 2867 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3932 4529 5316 5584 1239 Size: 393 2300 597 787 268 1524 Fragments arranged by size: 2300 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3178 3556 3178 Size: 378 5491 FspI TGC'GCA Cuts at: 2414 4375 2414 Size: 1961 3908 HaeII r_GCGC'y Cuts at: 1678 3004 5016 1678 Size: 1326 2012 2531 Fragments arranged by size: 2531 2012 1326 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2392 2424 3103 3135 4510 Size: 32 135 32 820 32 679 32 1375 Cuts at: 4510 4542 4584 4616 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1375 820 679 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2569 3213 3459 669 Size: 988 281 631 644 246 3079 Fragments arranged by size: 3079 988 644 631 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 3194 3194 Size: 5869 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4983 5167 1305 Size: 3678 184 2007 Fragments arranged by size: 3678 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2102 3102 10 Size: 54 1425 343 270 1000 2777 Fragments arranged by size: 2777 1425 1000 343 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 2795 3827 4186 4345 518 Size: 648 1629 1032 359 159 2042 Fragments arranged by size: 2042 1629 1032 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3285 3962 5110 5355 1606 Size: 1679 677 1148 245 2120 Fragments arranged by size: 2120 1679 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4378 Fragments arranged by size: 4378 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 2672 3074 3227 4772 1132 Size: 97 1443 402 153 1545 2229 Fragments arranged by size: 2229 1545 1443 402 153 97 PasI CC'CwG_GG Cuts at: 1348 2873 1348 Size: 1525 4344 PciI A'CATG_T Cuts at: 3070 4768 3070 Size: 1698 4171 PflMI CCAn_nnn'nTGG Cuts at: 1108 2383 1108 Size: 1275 4594 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2560 3938 3970 5451 5483 2528 Size: 32 1378 32 1481 32 2914 Fragments arranged by size: 2914 1481 1378 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PstI C_TGCA'G Cuts at: 830 3221 830 Size: 2391 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SbfI CC_TGCA'GG Cuts at: 3221 3221 Size: 5869 ScaI AGT'ACT Cuts at: 4117 5829 4117 Size: 1712 4157 SfcI C'TryA_G Cuts at: 826 1045 2389 3217 4352 5033 5224 826 Size: 219 1344 828 1135 681 191 1471 Fragments arranged by size: 1471 1344 1135 828 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 3196 1872 Size: 1324 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3973 4874 5136 820 Size: 197 926 2030 901 262 1553 Fragments arranged by size: 2030 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2550 3660 493 Size: 2057 1110 2702 Fragments arranged by size: 2702 2057 1110 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 2672 3227 1132 Size: 1540 555 3774 Fragments arranged by size: 3774 1540 555 SspI AAT'ATT Cuts at: 5 52 2842 3793 5 Size: 47 2790 951 2081 Fragments arranged by size: 2790 2081 951 47 StuI AGG'CCT Cuts at: 1517 2722 1517 Size: 1205 4664 StyI C'CwwG_G Cuts at: 513 1065 2004 3308 513 Size: 552 939 1304 3074 Fragments arranged by size: 3074 1304 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3896 3913 4066 4251 4696 325 Size: 1255 2316 17 153 185 445 1498 Fragments arranged by size: 2316 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3073 4115 5827 Size: 274 80 33 51 1443 1096 1042 1712 Cuts at: 5827 96 Size: 138 Fragments arranged by size: 1712 1443 1096 1042 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3101 3133 5829 5861 3101 Size: 32 2696 32 3109 Fragments arranged by size: 3109 2696 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5869 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 3194 1870 Size: 1324 4545 XmnI GAAnn'nnTTC Cuts at: 3013 3998 3013 Size: 985 4884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2305 3677 276 Size: 53 83 186 936 771 1372 2468 Fragments arranged by size: 2468 1372 936 771 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI