################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MroI MseI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 282 GTAC GT^AC Aor51HI 1033 AGCGCT AGC^GCT AscI 607 GGCGCGCC GG^CGCGCC AspHI 421 GTGCTC G[AT]GC[AT]^C BalI 1025 TGGCCA TGG^CCA BbsI 455 GTCTTC GAAGAC(2/6) BpuAI 455 GTCTTC GAAGAC(2/6) BsaI 73 GGTCTC GGTCTC(1/5) BseRI 1150 GAGGAG GAGGAG(10/8) BsiHKAI 421 GTGCTC G[AT]GC[AT]^C BsrI 980 ACTGG ACTGG(1/-1) BssSI 415 CTCGTG CACGAG(-5/-1) BstAPI 375 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 56 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 412 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 1057 CTCTTC CTCTTC(1/4) Eco47III 1033 AGCGCT AGC^GCT FseI 466 GGCCGGCC GGCCGG^CC HinfI 556 GACTC G^A[ACGT]TC HphI 1209 TCACC GGTGA(8/7) Ksp632I 1057 CTCTTC CTCTTC(1/4) MaeII 592 ACGT A^CGT MluNI 1025 TGGCCA TGG^CCA MscI 1025 TGGCCA TGG^CCA NlaIII 1213 CATG CATG^ NotI 949 GCGGCCGC GC^GGCCGC PaeR7I 433 CTCGAG C^TCGAG PflMI 411 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 556 GACTC GAGTC(4/5) PpuMI 353 GGGACCT [GA]G^G[AT]CC[CT] RsaI 282 GTAC GT^AC SbfI 684 CCTGCAGG CCTGCA^GG SfaNI 388 GCATC GCATC(5/9) SmaI 1160 CCCGGG CCC^GGG SmlI 433 CTCGAG C^T[CT][GA]AG Sse8387I 684 CCTGCAGG CCTGCA^GG Tsp45I 306 GTCAC ^GT[GC]AC Tsp509I 24 AATT ^AATT Van91I 411 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 347 CCACGGGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 433 CTCGAG C^TCGAG XmaCI 1160 CCCGGG C^CCGGG XmaI 1160 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 691 GTCTAC GT^[AC][GT]AC 1051 GTCTAC AflIII 284 ACGCGT A^C[GA][CT]GT 695 ACGCGT AlwI 6 GGATC GGATC(4/5) 656 GGATC AlwNI 376 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 874 CAGAGCCTG AvaI 433 CTCGAG C^[CT]CG[GA]G 1160 CCCGGG BbeI 36 GGCGCC GGCGC^C 400 GGCGCC BsgI 259 GTGCAG GTGCAG(16/14) 871 GTGCAG BsmAI 977 GAGAC GTCTC(1/5) 74 GTCTC BsoBI 433 CTCGAG C^[CT]CG[GA]G 1160 CCCGGG BspMI 683 ACCTGC ACCTGC(4/8) 687 GCAGGT BstF5I 153 CATCC GGATG(2/0) 389 CATCC BstYI 6 GGATCT [GA]^GATC[CT] 656 GGATCT DpnII 7 GATC ^GATC 657 GATC EcoT14I 86 CCTTGG C^C[AT][AT]GG 866 CCAAGG FokI 153 CATCC GGATG(9/13) 389 CATCC KasI 36 GGCGCC G^GCGCC 400 GGCGCC KspI 800 CCGCGG CCGC^GG 971 CCGCGG MaeIII 306 GTCAC ^GT[ACGT]AC 733 GTTAC MboI 7 GATC ^GATC 657 GATC MflI 6 GGATCT [GA]^GATC[CT] 656 GGATCT MluI 284 ACGCGT A^CGCGT 695 ACGCGT NarI 36 GGCGCC GG^CGCC 400 GGCGCC NdeII 7 GATC ^GATC 657 GATC SacII 800 CCGCGG CCGC^GG 971 CCGCGG Sau3AI 7 GATC ^GATC 657 GATC SfoI 36 GGCGCC GGC^GCC 400 GGCGCC SgrAI 32 CGCCGGCG C[GA]^CCGG[CT]G 439 CGCCGGCG StyI 86 CCTTGG C^C[AT][AT]GG 866 CCAAGG TaqI 434 TCGA T^CGA 834 TCGA TspRI 185 CAGTG CA[GC]TG[ACGT][ACGT]^ 515 CACTG TthHB8I 434 TCGA T^CGA 834 TCGA XhoII 6 GGATCT [GA]^GATC[CT] 656 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaII BanI BanII BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmFI Bsp1286I BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIV PstI PvuII Sau96I ScrFI SfcI TseI