(Circular) (Six-base) MAPSORT of: pFN21AE0643.seq Check: 8101 from: 1 to: 6007 pFN21AE0643 6007 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2697 2697 Size: 6007 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2697 3057 3350 1937 Size: 760 360 293 4594 Fragments arranged by size: 4594 760 360 293 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2906 4070 5454 5722 1632 Size: 1274 1164 1384 268 1917 Fragments arranged by size: 1917 1384 1274 1164 268 AfeI AGC'GCT Cuts at: 3040 3040 Size: 6007 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 2289 2700 4906 5883 1203 Size: 1086 411 2206 977 1327 Fragments arranged by size: 2206 1327 1086 977 411 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 2189 1166 Size: 1023 4984 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AlwNI CAG_nnn'CTG Cuts at: 2386 2884 3750 5322 2386 Size: 498 866 1572 3071 Fragments arranged by size: 3071 1572 866 498 ApaI G_GGCC'C Cuts at: 2223 2234 2976 3165 2223 Size: 11 742 189 5065 Fragments arranged by size: 5065 742 189 11 ApaLI G'TGCA_C Cuts at: 4064 5220 4064 Size: 1156 4851 ApoI r'AATT_y Cuts at: 1493 3316 3542 3694 5803 1493 Size: 1823 226 152 2109 1697 Fragments arranged by size: 2109 1823 1697 226 152 AscI GG'CGCG_CC Cuts at: 2613 2613 Size: 6007 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2438 3165 3332 1870 Size: 73 495 727 167 4545 Fragments arranged by size: 4545 727 495 167 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2405 2685 2787 3328 Size: 325 217 627 254 364 280 102 541 Cuts at: 3328 4783 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 541 364 325 280 254 217 102 BanII G_rGCy'C Cuts at: 1391 2107 2223 2234 2343 2834 2845 2976 3119 Size: 716 116 11 109 491 11 131 143 Cuts at: 3119 3165 3326 1391 Size: 46 161 4072 Fragments arranged by size: 4072 716 491 161 143 131 116 109 46 11 11 BbeI G_GCGC'C Cuts at: 2045 2409 2045 Size: 364 5643 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2453 928 Size: 971 554 4482 Fragments arranged by size: 4482 971 554 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4198 4232 1903 Size: 34 2261 34 3678 Fragments arranged by size: 3678 2261 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2208 2683 2807 Size: 107 122 71 631 605 536 475 124 Cuts at: 2807 2951 4618 136 Size: 144 1667 1525 Fragments arranged by size: 1667 1525 631 605 536 475 144 124 122 107 71 BglII A'GATC_T Cuts at: 6002 6002 Size: 6007 BlpI GC'TnA_GC Cuts at: 3424 3424 Size: 6007 Bme1580I G_kGCm'C Cuts at: 1790 2061 2223 2234 2303 2494 2976 3165 4068 Size: 271 162 11 69 191 482 189 903 Cuts at: 4068 5224 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 903 482 271 191 189 162 69 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2995 3738 4696 Size: 416 53 83 399 24 1578 743 958 Cuts at: 4696 442 Size: 1753 Fragments arranged by size: 1753 1578 958 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4667 1239 Size: 3428 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4132 4997 5295 4132 Size: 865 298 4844 Fragments arranged by size: 4844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 4670 882 Size: 617 585 2586 2219 Fragments arranged by size: 2586 2219 617 585 BsaAI yAC'GTr Cuts at: 493 1204 3798 493 Size: 711 2594 2702 Fragments arranged by size: 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2406 2751 3814 Size: 53 83 186 936 509 364 345 1063 Cuts at: 3814 4196 4831 275 Size: 382 635 1451 Fragments arranged by size: 1451 1063 936 635 509 382 364 345 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2756 2786 1178 Size: 30 165 30 1353 30 4399 Fragments arranged by size: 4399 1353 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3170 1186 Size: 1984 4023 BseYI C'CCAG_C Cuts at: 2068 2073 2123 2332 2852 3112 5210 5706 2068 Size: 5 50 209 520 260 2098 496 2369 Fragments arranged by size: 2369 2098 520 496 260 209 50 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 2897 1163 Size: 573 549 612 4273 Fragments arranged by size: 4273 612 573 549 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2473 2477 2625 2795 2958 3457 Size: 275 186 472 4 148 170 163 499 Cuts at: 3457 4218 5246 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 499 472 275 186 170 163 148 4 BsiHKAI G_wGCw'C Cuts at: 2430 4068 4153 5224 2430 Size: 1638 85 1071 3213 Fragments arranged by size: 3213 1638 1071 85 BsmI GAATG_Cn' Cuts at: 3518 3611 3518 Size: 93 5914 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2107 2223 2234 2303 2343 2430 Size: 399 271 46 116 11 69 40 87 Cuts at: 2430 2494 2834 2845 2976 3119 3165 3326 4068 Size: 64 340 11 131 143 46 161 742 Cuts at: 4068 4153 5224 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 340 271 161 143 131 116 87 85 69 64 46 46 40 11 11 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 3896 5626 3896 Size: 1730 4277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2683 2697 3362 844 Size: 1839 14 665 3489 Fragments arranged by size: 3489 1839 665 14 BsrBI CCG'CTC Cuts at: 1394 1823 3894 1394 Size: 429 2071 3507 Fragments arranged by size: 3507 2071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 2445 2472 2790 3084 4651 4838 Size: 110 228 407 27 318 294 1567 187 Cuts at: 4838 1700 Size: 2869 Fragments arranged by size: 2869 1567 407 318 294 228 187 110 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6007 BssHII G'CGCG_C Cuts at: 1925 2449 2613 2856 3129 1925 Size: 524 164 243 273 4803 Fragments arranged by size: 4803 524 273 243 164 BssSI C'ACGA_G Cuts at: 2420 4067 5079 2420 Size: 1647 1012 3348 Fragments arranged by size: 3348 1647 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2386 794 Size: 1592 4415 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstXI CCAn_nnnn'nTGG Cuts at: 2068 2068 Size: 6007 BstYI r'GATC_y Cuts at: 1972 2011 2661 3337 3704 4089 4106 5558 5644 Size: 39 650 676 367 385 17 1452 86 Cuts at: 5644 5656 6002 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 676 650 385 367 346 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2352 2656 2805 2976 513 Size: 552 939 348 304 149 171 3544 Fragments arranged by size: 3544 939 552 348 304 171 149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2416 3041 487 Size: 683 56 1190 625 3453 Fragments arranged by size: 3453 1190 683 625 56 BtsI GCAGTG_nn' Cuts at: 2519 3523 4317 4337 2519 Size: 1004 794 20 4189 Fragments arranged by size: 4189 1004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DraIII CAC_nnn'GTG Cuts at: 2422 2422 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2470 2474 Size: 54 1425 50 275 18 519 121 4 Cuts at: 2474 2622 2636 2761 2792 2955 3030 3454 4343 Size: 148 14 125 31 163 75 424 889 Cuts at: 4343 5972 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 519 424 275 163 148 125 121 75 54 50 43 31 18 14 4 EagI C'GGCC_G Cuts at: 1537 1812 2470 2474 2622 2792 2955 3454 1537 Size: 275 658 4 148 170 163 499 4090 Fragments arranged by size: 4090 658 499 275 170 163 148 4 EarI CTCTTCn'nnn_ Cuts at: 1353 3068 3937 1353 Size: 1715 869 3423 Fragments arranged by size: 3423 1715 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2126 2294 4594 4968 5114 387 Size: 1064 138 537 168 2300 374 146 1280 Fragments arranged by size: 2300 1280 1064 537 374 168 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2906 4070 4667 5454 5722 1239 Size: 393 1274 1164 597 787 268 1524 Fragments arranged by size: 1524 1274 1164 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2220 2231 2359 2800 2998 3161 3162 2220 Size: 11 128 441 198 163 1 5065 Fragments arranged by size: 5065 441 198 163 128 11 1 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 2269 Size: 6007 FseI GG_CCGG'CC Cuts at: 2476 2476 Size: 6007 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 2045 2309 2409 2446 2534 2819 2909 3014 Size: 367 264 100 37 88 285 90 105 Cuts at: 3014 3042 3182 5154 1678 Size: 28 140 1972 2531 Fragments arranged by size: 2531 1972 367 285 264 140 105 100 90 88 37 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 2821 2853 2987 Size: 32 135 32 980 32 237 32 134 Cuts at: 2987 3019 4648 4680 4722 4754 1373 Size: 32 1629 32 42 32 2626 Fragments arranged by size: 2626 1629 980 237 135 134 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3351 3597 669 Size: 988 281 1413 246 3079 Fragments arranged by size: 3079 1413 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KasI G'GCGC_C Cuts at: 2041 2405 2041 Size: 364 5643 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 2289 2700 5883 2289 Size: 411 3183 2413 Fragments arranged by size: 3183 2413 411 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5121 5305 1305 Size: 3816 184 2007 Fragments arranged by size: 3816 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3032 10 Size: 54 1425 343 1200 2985 Fragments arranged by size: 2985 1425 1200 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 2162 2189 3965 4324 4483 518 Size: 648 888 108 27 1776 359 159 2042 Fragments arranged by size: 2042 1776 888 648 359 159 108 27 MspA1I CmG'CkG Cuts at: 1606 2269 2317 2383 2479 2575 2807 2978 3100 Size: 663 48 66 96 96 232 171 122 Cuts at: 3100 3235 3423 4100 5248 5493 1606 Size: 135 188 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 663 245 232 188 171 135 122 96 96 66 48 NaeI GCC'GGC Cuts at: 2040 2447 2474 3086 2040 Size: 407 27 612 4961 Fragments arranged by size: 4961 612 407 27 NarI GG'CG_CC Cuts at: 2042 2406 2042 Size: 364 5643 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4516 Fragments arranged by size: 4516 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NgoMIV G'CCGG_C Cuts at: 2038 2445 2472 3084 2038 Size: 407 27 612 4961 Fragments arranged by size: 4961 612 407 27 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 2955 3454 2955 Size: 499 5508 NspI r_CATG'y Cuts at: 1132 1229 3365 4910 1132 Size: 97 2136 1545 2229 Fragments arranged by size: 2229 2136 1545 97 PasI CC'CwG_GG Cuts at: 1348 2223 2503 1348 Size: 875 280 4852 Fragments arranged by size: 4852 875 280 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 2422 1108 Size: 1314 4693 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2359 Size: 6007 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PspOMI G'GGCC_C Cuts at: 2219 2230 2972 3161 2219 Size: 11 742 189 5065 Fragments arranged by size: 5065 742 189 11 PspXI vC'TCGA_Gb Cuts at: 2438 2438 Size: 6007 PstI C_TGCA'G Cuts at: 830 2382 2391 2607 2694 2815 3359 830 Size: 1552 9 216 87 121 544 3478 Fragments arranged by size: 3478 1552 544 216 121 87 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2269 2317 2383 3100 2269 Size: 48 66 717 5176 Fragments arranged by size: 5176 717 66 48 SacII CC_GC'GG Cuts at: 2808 2979 2808 Size: 171 5836 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SbfI CC_TGCA'GG Cuts at: 2694 3359 2694 Size: 665 5342 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2378 2387 2603 2690 2811 3355 4490 Size: 219 1333 9 216 87 121 544 1135 Cuts at: 4490 5171 5362 826 Size: 681 191 1471 Fragments arranged by size: 1471 1333 1135 681 544 219 216 191 121 87 9 SfoI GGC'GCC Cuts at: 2043 2407 2043 Size: 364 5643 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 2038 2445 1700 Size: 338 407 5262 Fragments arranged by size: 5262 407 338 SmaI CCC'GGG Cuts at: 1872 3167 3334 1872 Size: 1295 167 4545 Fragments arranged by size: 4545 1295 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 4111 5012 5274 820 Size: 197 926 495 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 495 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6007 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 2871 3446 513 Size: 552 939 87 780 575 3074 Fragments arranged by size: 3074 939 780 575 552 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4034 4051 4204 4389 4834 325 Size: 1255 2454 17 153 185 445 1498 Fragments arranged by size: 2454 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4253 5965 96 Size: 274 80 33 51 1443 2276 1712 138 Fragments arranged by size: 2276 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2351 2383 2756 2788 5967 5999 2351 Size: 32 373 32 3179 32 2359 Fragments arranged by size: 3179 2359 373 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6007 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2359 Size: 6007 XhoI C'TCGA_G Cuts at: 1943 2438 1943 Size: 495 5512 XmaI C'CCGG_G Cuts at: 1870 3165 3332 1870 Size: 1295 167 4545 Fragments arranged by size: 4545 1295 167 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI