(Linear) (Six-base) MAPSORT of: KIEE0643.seq Check: 9273 from: 1 to: 1302 KIEE0643.orf With 182 enzymes: SgfI * December 27, 2011 16:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 692 1302 Size: 692 610 AccI GT'mk_AC Cuts at: 0 692 1052 1302 Size: 692 360 250 Fragments arranged by size: 692 360 250 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 901 1302 Size: 901 401 AfeI AGC'GCT Cuts at: 0 1035 1302 Size: 1035 267 AflIII A'CryG_T Cuts at: 0 284 695 1302 Size: 284 411 607 Fragments arranged by size: 607 411 284 AleI CACnn'nnGTG Cuts at: 0 184 1302 Size: 184 1118 AlwNI CAG_nnn'CTG Cuts at: 0 381 879 1302 Size: 381 498 423 Fragments arranged by size: 498 423 381 ApaI G_GGCC'C Cuts at: 0 218 229 971 1160 1302 Size: 218 11 742 189 142 Fragments arranged by size: 742 218 189 142 11 AscI GG'CGCG_CC Cuts at: 0 608 1302 Size: 608 694 AvaI C'yCGr_G Cuts at: 0 433 1160 1302 Size: 433 727 142 Fragments arranged by size: 727 433 142 BanI G'GyrC_C Cuts at: 0 36 400 680 782 1302 Size: 36 364 280 102 520 Fragments arranged by size: 520 364 280 102 36 BanII G_rGCy'C Cuts at: 0 102 218 229 338 829 840 971 1114 Size: 102 116 11 109 491 11 131 143 Cuts at: 1114 1160 1302 Size: 46 142 Fragments arranged by size: 491 143 142 131 116 109 102 46 11 11 BbeI G_GCGC'C Cuts at: 0 40 404 1302 Size: 40 364 898 Fragments arranged by size: 898 364 40 BbsI GAAGACnn'nnnn_ Cuts at: 0 448 1302 Size: 448 854 BglI GCCn_nnn'nGGC Cuts at: 0 203 678 802 946 1302 Size: 203 475 124 144 356 Fragments arranged by size: 475 356 203 144 124 Bme1580I G_kGCm'C Cuts at: 0 56 218 229 298 489 971 1160 1302 Size: 56 162 11 69 191 482 189 142 Fragments arranged by size: 482 191 189 162 142 69 56 11 BmrI ACTGGGnnnn_n' Cuts at: 0 990 1302 Size: 990 312 BsaI GGTCTCn'nnnn_ Cuts at: 0 79 1302 Size: 79 1223 BsaHI Gr'CG_yC Cuts at: 0 37 401 746 1302 Size: 37 364 345 556 Fragments arranged by size: 556 364 345 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 751 781 1302 Size: 751 30 521 Fragments arranged by size: 751 521 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1165 1302 Size: 1165 137 BseYI C'CCAG_C Cuts at: 0 63 68 118 327 847 1107 1302 Size: 63 5 50 209 520 260 195 Fragments arranged by size: 520 260 209 195 63 50 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 892 1302 Size: 280 612 410 Fragments arranged by size: 612 410 280 BsiEI CG_ry'CG Cuts at: 0 468 472 620 790 953 1302 Size: 468 4 148 170 163 349 Fragments arranged by size: 468 349 170 163 148 4 BsiHKAI G_wGCw'C Cuts at: 0 425 1302 Size: 425 877 Bsp1286I G_dGCh'C Cuts at: 0 56 102 218 229 298 338 425 489 Size: 56 46 116 11 69 40 87 64 Cuts at: 489 829 840 971 1114 1160 1302 Size: 340 11 131 143 46 142 Fragments arranged by size: 340 143 142 131 116 87 69 64 56 46 46 40 11 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 678 692 1302 Size: 678 14 610 Fragments arranged by size: 678 610 14 BsrFI r'CCGG_y Cuts at: 0 33 440 467 785 1079 1302 Size: 33 407 27 318 294 223 Fragments arranged by size: 407 318 294 223 33 27 BssHII G'CGCG_C Cuts at: 0 444 608 851 1124 1302 Size: 444 164 243 273 178 Fragments arranged by size: 444 273 243 178 164 BssSI C'ACGA_G Cuts at: 0 415 1302 Size: 415 887 BstAPI GCAn_nnn'nTGC Cuts at: 0 381 1302 Size: 381 921 BstXI CCAn_nnnn'nTGG Cuts at: 0 63 1302 Size: 63 1239 BstYI r'GATC_y Cuts at: 0 6 656 1302 Size: 6 650 646 Fragments arranged by size: 650 646 6 BtgI C'CryG_G Cuts at: 0 347 651 800 971 1302 Size: 347 304 149 171 331 Fragments arranged by size: 347 331 304 171 149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 411 1036 1302 Size: 411 625 266 Fragments arranged by size: 625 411 266 BtsI GCAGTG_nn' Cuts at: 0 514 1302 Size: 514 788 DraIII CAC_nnn'GTG Cuts at: 0 417 1302 Size: 417 885 EaeI y'GGCC_r Cuts at: 0 344 465 469 617 631 756 787 950 Size: 344 121 4 148 14 125 31 163 Cuts at: 950 1025 1302 Size: 75 277 Fragments arranged by size: 344 277 163 148 125 121 75 31 14 4 EagI C'GGCC_G Cuts at: 0 465 469 617 787 950 1302 Size: 465 4 148 170 163 352 Fragments arranged by size: 465 352 170 163 148 4 EarI CTCTTCn'nnn_ Cuts at: 0 1063 1302 Size: 1063 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 121 289 1302 Size: 121 168 1013 Fragments arranged by size: 1013 168 121 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 901 1302 Size: 901 401 EcoO109I rG'GnC_Cy Cuts at: 0 215 226 354 795 993 1156 1157 1302 Size: 215 11 128 441 198 163 1 145 Fragments arranged by size: 441 215 198 163 145 128 11 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 264 1302 Size: 264 1038 FseI GG_CCGG'CC Cuts at: 0 471 1302 Size: 471 831 HaeII r_GCGC'y Cuts at: 0 40 304 404 441 529 814 904 1009 Size: 40 264 100 37 88 285 90 105 Cuts at: 1009 1037 1177 1302 Size: 28 140 125 Fragments arranged by size: 285 264 140 125 105 100 90 88 40 37 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 547 579 816 848 982 1014 1302 Size: 547 32 237 32 134 32 288 Fragments arranged by size: 547 288 237 134 32 32 32 KasI G'GCGC_C Cuts at: 0 36 400 1302 Size: 36 364 902 Fragments arranged by size: 902 364 36 MluI A'CGCG_T Cuts at: 0 284 695 1302 Size: 284 411 607 Fragments arranged by size: 607 411 284 MscI TGG'CCA Cuts at: 0 1027 1302 Size: 1027 275 MslI CAynn'nnrTG Cuts at: 0 49 157 184 1302 Size: 49 108 27 1118 Fragments arranged by size: 1118 108 49 27 MspA1I CmG'CkG Cuts at: 0 264 312 378 474 570 802 973 1095 Size: 264 48 66 96 96 232 171 122 Cuts at: 1095 1230 1302 Size: 135 72 Fragments arranged by size: 264 232 171 135 122 96 96 72 66 48 NaeI GCC'GGC Cuts at: 0 35 442 469 1081 1302 Size: 35 407 27 612 221 Fragments arranged by size: 612 407 221 35 27 NarI GG'CG_CC Cuts at: 0 37 401 1302 Size: 37 364 901 Fragments arranged by size: 901 364 37 NgoMIV G'CCGG_C Cuts at: 0 33 440 467 1079 1302 Size: 33 407 27 612 223 Fragments arranged by size: 612 407 223 33 27 NotI GC'GGCC_GC Cuts at: 0 950 1302 Size: 950 352 PasI CC'CwG_GG Cuts at: 0 218 498 1302 Size: 218 280 804 Fragments arranged by size: 804 280 218 PflMI CCAn_nnn'nTGG Cuts at: 0 417 1302 Size: 417 885 PpuMI rG'GwC_Cy Cuts at: 0 354 1302 Size: 354 948 PspOMI G'GGCC_C Cuts at: 0 214 225 967 1156 1302 Size: 214 11 742 189 146 Fragments arranged by size: 742 214 189 146 11 PspXI vC'TCGA_Gb Cuts at: 0 433 1302 Size: 433 869 PstI C_TGCA'G Cuts at: 0 377 386 602 689 810 1302 Size: 377 9 216 87 121 492 Fragments arranged by size: 492 377 216 121 87 9 PvuII CAG'CTG Cuts at: 0 264 312 378 1095 1302 Size: 264 48 66 717 207 Fragments arranged by size: 717 264 207 66 48 SacII CC_GC'GG Cuts at: 0 803 974 1302 Size: 803 171 328 Fragments arranged by size: 803 328 171 SbfI CC_TGCA'GG Cuts at: 0 689 1302 Size: 689 613 SfcI C'TryA_G Cuts at: 0 373 382 598 685 806 1302 Size: 373 9 216 87 121 496 Fragments arranged by size: 496 373 216 121 87 9 SfoI GGC'GCC Cuts at: 0 38 402 1302 Size: 38 364 900 Fragments arranged by size: 900 364 38 SgrAI Cr'CCGG_yG Cuts at: 0 33 440 1302 Size: 33 407 862 Fragments arranged by size: 862 407 33 SmaI CCC'GGG Cuts at: 0 1162 1302 Size: 1162 140 SmlI C'TyrA_G Cuts at: 0 433 1302 Size: 433 869 StyI C'CwwG_G Cuts at: 0 86 866 1302 Size: 86 780 436 Fragments arranged by size: 780 436 86 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 346 378 751 783 1302 Size: 346 32 373 32 519 Fragments arranged by size: 519 373 346 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 354 1302 Size: 354 948 XhoI C'TCGA_G Cuts at: 0 433 1302 Size: 433 869 XmaI C'CCGG_G Cuts at: 0 1160 1302 Size: 1160 142 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AleI AlwNI ApaI AscI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmrI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I FalI FseI HaeII Hin4I KasI MluI MscI MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI PpuMI PspOMI PspXI PstI PvuII SacII SbfI SfcI SfoI SgrAI SmaI SmlI StyI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI MmeI NcoI NdeI NheI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XmnI ZraI